FastQCFastQC Report
Thu 26 May 2016
SRR1545183_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545183_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2070248
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118150.5707045725922691No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC35310.17055927599012294No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG207.034599E-445.019
CGCATCG850.045.021
TATATCG351.2125565E-745.01
TCGATAG700.045.01
TCTAGCG1000.045.01
AACGCAT351.2125565E-745.026
CGTACTC406.8193913E-945.030
TATAGCG1100.045.01
CACCGTT207.034599E-445.010
TTATTCG351.2125565E-745.019
TGTCGTA351.2125565E-745.042
TAGTCCG406.8193913E-945.01
CGAACGC302.166349E-644.99999615
GTCGATA302.166349E-644.99999621
CACAACG1050.044.99999612
CGTTTTT74850.042.865731
CGACGGT5700.042.2368428
CGGTCTA5750.041.47826431
TCGACGG1250.041.3999982
CGTTAGG3700.041.3513532
Produced by FastQC (version 0.11.5)