##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545183_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2070248
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.05192662908019	31.0	31.0	33.0	30.0	34.0
2	31.334235801701052	31.0	31.0	34.0	30.0	34.0
3	31.444351111557648	31.0	31.0	34.0	30.0	34.0
4	35.21448493127394	37.0	35.0	37.0	33.0	37.0
5	35.061639475077385	37.0	35.0	37.0	32.0	37.0
6	35.12354075453762	37.0	35.0	37.0	32.0	37.0
7	35.75025166067061	37.0	35.0	37.0	35.0	37.0
8	35.757230534699225	37.0	35.0	37.0	35.0	37.0
9	37.44857427709144	39.0	37.0	39.0	35.0	39.0
10	36.610878986478916	39.0	35.0	39.0	32.0	39.0
11	36.302353148028644	38.0	35.0	39.0	32.0	39.0
12	35.60983853142232	37.0	35.0	39.0	30.0	39.0
13	35.27963074955271	37.0	35.0	39.0	30.0	39.0
14	36.2524282114993	38.0	35.0	40.0	30.0	41.0
15	36.53968413446118	38.0	35.0	40.0	31.0	41.0
16	36.66255347185458	38.0	35.0	40.0	31.0	41.0
17	36.61051960924488	38.0	35.0	40.0	31.0	41.0
18	36.53398530031185	38.0	35.0	40.0	31.0	41.0
19	36.47043639216171	38.0	35.0	40.0	31.0	41.0
20	36.32629858838168	38.0	35.0	40.0	30.0	41.0
21	36.202400388745694	38.0	34.0	40.0	30.0	41.0
22	36.10506180901998	38.0	34.0	40.0	30.0	41.0
23	36.06061254497046	38.0	34.0	40.0	30.0	41.0
24	35.980103832970734	37.0	34.0	40.0	30.0	41.0
25	35.90432788728693	37.0	34.0	40.0	30.0	41.0
26	35.7644567220932	37.0	34.0	40.0	30.0	41.0
27	35.665730385924775	37.0	34.0	40.0	29.0	41.0
28	35.67212672104984	37.0	34.0	40.0	29.0	41.0
29	35.635287173324166	37.0	34.0	40.0	29.0	41.0
30	35.55898713584073	37.0	34.0	40.0	29.0	41.0
31	35.431555060070096	37.0	34.0	40.0	29.0	41.0
32	35.27335372380507	37.0	34.0	40.0	28.0	41.0
33	35.071587558592014	37.0	34.0	40.0	27.0	41.0
34	34.919403375827436	37.0	34.0	40.0	26.0	41.0
35	34.79365322415479	37.0	33.0	40.0	26.0	41.0
36	34.6214530819496	37.0	33.0	40.0	24.0	41.0
37	34.59914029623504	36.0	33.0	40.0	25.0	41.0
38	34.573508584478766	36.0	33.0	40.0	25.0	41.0
39	34.52993409485241	36.0	33.0	40.0	24.0	41.0
40	34.41871263732654	36.0	33.0	40.0	24.0	41.0
41	34.38175184808777	36.0	33.0	40.0	24.0	41.0
42	34.29633164722294	36.0	33.0	40.0	24.0	41.0
43	34.24409587643606	36.0	33.0	40.0	24.0	41.0
44	34.106722962659546	35.0	33.0	40.0	23.0	41.0
45	34.048036756948925	35.0	33.0	40.0	23.0	41.0
46	33.953920979515495	35.0	33.0	40.0	23.0	41.0
47	33.87016289835807	35.0	33.0	40.0	23.0	41.0
48	33.82779019711648	35.0	33.0	39.0	23.0	41.0
49	33.811806121778645	35.0	33.0	39.0	24.0	41.0
50	33.74133871883948	35.0	33.0	39.0	24.0	41.0
51	33.580314049331285	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	3.0
12	0.0
13	4.0
14	18.0
15	69.0
16	207.0
17	560.0
18	1241.0
19	2268.0
20	3963.0
21	6393.0
22	9749.0
23	13943.0
24	19781.0
25	27189.0
26	35794.0
27	42243.0
28	47254.0
29	55087.0
30	65957.0
31	80825.0
32	98478.0
33	123931.0
34	189399.0
35	258661.0
36	162617.0
37	193809.0
38	257445.0
39	373121.0
40	236.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.88263048678226	24.068758911975763	29.487529996406224	13.561080604835748
2	30.787712390013176	25.68624628546918	30.730666084449787	12.795375240067857
3	29.18867691213806	24.743557293618927	32.369406950278425	13.698358843964586
4	26.27979836232181	27.372421081918684	31.460675242772844	14.887105312986657
5	22.06523083224812	32.26898419899452	31.272340318647814	14.393444650109553
6	19.82506443672449	42.763089253074995	27.38208176025288	10.02976454994764
7	87.5409129727453	4.226595074599758	6.550664461455826	1.681827491199122
8	88.94460953470309	3.0770709596145003	6.220897206518253	1.7574222991641582
9	84.14822765195281	5.492192239770308	7.71323049219224	2.646349616084643
10	43.592941521981906	31.61795108605346	13.541952461734052	11.24715493023058
11	39.54511729995633	22.265544997507543	23.93896769855592	14.250370003980201
12	37.395036729899026	21.45235739872711	25.743002770682544	15.409603100691319
13	23.119355748683247	35.34900166550094	24.837845514160623	16.693797071655183
14	17.038248557660722	37.23703633574335	27.413527268230663	18.31118783836526
15	15.94475637701377	24.489626363604746	41.47135995301046	18.094257306371027
16	18.568548309188078	20.673030477508007	39.372915708649394	21.385505504654514
17	17.686528377276538	21.441295922034463	29.628624203477074	31.24355149721193
18	21.08771509500311	25.23707304632102	32.83369915101959	20.841512707656282
19	27.717162388274254	25.782225124719353	24.877164474980777	21.623448012025612
20	28.987203465478533	24.93136088043558	25.62176125758846	20.459674396497423
21	22.494841197769546	28.97556234808583	27.66460829813626	20.864988156008362
22	23.300541770840983	25.35048940996441	25.433909367380142	25.91505945181447
23	20.32312070824365	29.56027490426268	26.212946468249214	23.90365791924446
24	19.65885246598475	24.41808904054007	37.266549708054306	18.65650878542088
25	19.262619744107955	26.90605183533567	31.900622534111854	21.930705886444525
26	18.219363090798783	32.373029704653746	26.768387169073467	22.63922003547401
27	17.804436956345327	32.657005344287256	28.271830234831768	21.266727464535652
28	17.07003218938021	28.570393498749908	35.03860407062342	19.32097024124646
29	18.048417387675293	25.359908571340245	33.50468156472075	23.086992476263713
30	20.234242467569103	30.60918305439735	29.699823402800053	19.4567510752335
31	27.704361989481452	27.58481109509585	25.190943307275266	19.519883608147428
32	28.74781185635731	26.65419795116334	25.557276229707742	19.040713962771612
33	26.253134890119444	28.513830227103227	24.643617576251735	20.589417306525597
34	19.249046491048418	27.710448216831995	28.76930686565088	24.27119842646871
35	20.710320695877982	26.386983588439644	30.583099222895033	22.319596492787337
36	27.07897797751767	27.37623704986069	26.2939512560814	19.250833716540242
37	19.672812146177655	33.5544823615335	27.41782627008938	19.354879222199468
38	19.888438486596772	32.58822131454782	26.117933696832456	21.40540650202295
39	20.170530293955117	31.922503970538795	26.567589969897327	21.339375765608757
40	23.546454337837787	27.62987815952485	26.253231496902785	22.570436005734578
41	18.362872467453176	25.615433513279566	27.525301316557243	28.496392702710015
42	19.626923924090253	27.445890540650204	25.463809166824458	27.46337636843509
43	20.841174583914583	26.620916914302057	26.528319312468845	26.009589189314518
44	19.656437296401204	28.55739988639042	29.0883507676375	22.697812049570874
45	18.344468875226543	34.95083680795731	24.28525471344496	22.419439603371192
46	20.624099141745337	33.36286280677484	25.849994783233697	20.163043268246124
47	19.977654851013018	28.18449770269069	27.76817076987878	24.06967667641751
48	20.316213323234706	26.852000340055877	31.14496427481152	21.6868220618979
49	20.815380572762297	26.211545669890757	30.79949841758089	22.173575339766057
50	19.30324289650322	31.174477647122472	28.075235430731006	21.447044025643304
51	18.751231736487608	30.923010190083506	26.871708123857623	23.45404994957126
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1234.0
1	2010.0
2	2786.0
3	10782.5
4	18779.0
5	13388.5
6	7998.0
7	8522.0
8	9046.0
9	9675.0
10	10304.0
11	10603.0
12	10902.0
13	10965.5
14	11029.0
15	10291.0
16	9553.0
17	8794.5
18	8036.0
19	7917.0
20	7798.0
21	7987.0
22	8176.0
23	8198.0
24	8220.0
25	9652.0
26	15293.5
27	19503.0
28	23607.5
29	27712.0
30	32379.0
31	37046.0
32	43794.5
33	50543.0
34	60953.5
35	71364.0
36	72676.5
37	73989.0
38	79666.5
39	85344.0
40	111509.5
41	137675.0
42	158670.0
43	179665.0
44	182671.0
45	185677.0
46	179346.0
47	173015.0
48	165957.0
49	158899.0
50	147663.5
51	136428.0
52	126624.5
53	116821.0
54	108506.0
55	100191.0
56	95560.5
57	90930.0
58	85723.0
59	80516.0
60	73737.5
61	66959.0
62	57841.0
63	48723.0
64	43004.5
65	37286.0
66	29115.5
67	20945.0
68	18233.0
69	15521.0
70	13756.5
71	11992.0
72	10381.0
73	8770.0
74	7038.0
75	3782.5
76	2259.0
77	1703.0
78	1147.0
79	778.5
80	410.0
81	433.5
82	457.0
83	298.0
84	139.0
85	88.5
86	38.0
87	27.0
88	16.0
89	13.0
90	10.0
91	7.5
92	5.0
93	3.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2070248.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.109005708512843
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.8606801194888	14.28088506461028
2	8.082804993830738	2.927431235481539
3	2.889922039398736	1.5700084412588637
4	1.4922220964376072	1.080906338510305
5	0.941613548328357	0.8525842560945626
6	0.6144372960609331	0.667610910113438
7	0.4546756551147096	0.5763606823795865
8	0.3870316325162608	0.560700643408961
9	0.29578479459657964	0.48207316804566824
>10	3.5139430627636408	14.841409772823116
>50	1.118428111592867	14.597035191291152
>100	1.3356472457174318	45.27223761092853
>500	0.010941366047616093	1.24580010514338
>1k	0.0016011755191633304	0.473982987363601
>5k	0.0	0.0
>10k+	2.6686258652722174E-4	0.570973592546997
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11815	0.5707045725922691	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3531	0.17055927599012294	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03337764364462615	0.0
2	0.0	0.0	0.0	0.12534730138611413	0.0
3	0.0	0.0	0.0	0.18239360694950557	0.0
4	0.0	0.0	0.0	0.2678423068154154	0.0
5	0.0	0.0	0.0	0.4629880091660516	0.0
6	0.0	0.0	0.0	0.6806430920353503	0.0
7	0.0	0.0	0.0	0.8322191350987901	0.0
8	0.0	0.0	0.0	1.2294662282006794	0.0
9	0.0	0.0	0.0	1.4075608332914704	0.0
10	0.0	0.0	0.0	1.7068486480846738	0.0
11	0.0	0.0	0.0	1.9274985412375716	0.0
12	0.0	0.0	0.0	2.139647037456382	0.0
13	0.0	0.0	0.0	2.2245644000139113	0.0
14	0.0	0.0	0.0	2.2556717842500027	0.0
15	0.0	0.0	0.0	2.3039751759209524	0.0
16	0.0	0.0	0.0	2.4143967292807433	0.0
17	0.0	0.0	0.0	2.5441879427005847	0.0
18	0.0	0.0	0.0	2.743680950301606	0.0
19	0.0	0.0	0.0	2.818937634524946	0.0
20	0.0	0.0	0.0	2.904048210649159	0.0
21	0.0	0.0	0.0	3.0050989060247852	0.0
22	0.0	0.0	0.0	3.1124773457093062	0.0
23	0.0	0.0	0.0	3.231255385828171	0.0
24	0.0	0.0	0.0	3.3279104725617414	0.0
25	0.0	0.0	0.0	3.4080941027355176	0.0
26	0.0	0.0	0.0	3.4875531820342296	0.0
27	0.0	0.0	0.0	3.569668947874844	0.0
28	0.0	0.0	0.0	3.6534753324239415	0.0
29	0.0	0.0	0.0	3.7454449901654296	0.0
30	4.830339167094957E-5	0.0	0.0	3.8715168424266078	0.0
31	4.830339167094957E-5	0.0	0.0	3.9723260208438798	0.0
32	9.660678334189913E-5	0.0	0.0	4.069850568627527	0.0
33	1.9321356668379827E-4	0.0	0.0	4.164042182385878	0.0
34	1.9321356668379827E-4	0.0	0.0	4.264320023494769	0.0
35	1.9321356668379827E-4	0.0	0.0	4.403675308465459	0.0
36	1.9321356668379827E-4	0.0	0.0	4.510087680316561	0.0
37	1.9321356668379827E-4	0.0	0.0	4.615002647025864	0.0
38	1.9321356668379827E-4	0.0	0.0	4.721076895135269	0.0
39	1.9321356668379827E-4	0.0	0.0	4.8402896657791725	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGACG	20	7.034599E-4	45.0	19
CGCATCG	85	0.0	45.0	21
TATATCG	35	1.2125565E-7	45.0	1
TCGATAG	70	0.0	45.0	1
TCTAGCG	100	0.0	45.0	1
AACGCAT	35	1.2125565E-7	45.0	26
CGTACTC	40	6.8193913E-9	45.0	30
TATAGCG	110	0.0	45.0	1
CACCGTT	20	7.034599E-4	45.0	10
TTATTCG	35	1.2125565E-7	45.0	19
TGTCGTA	35	1.2125565E-7	45.0	42
TAGTCCG	40	6.8193913E-9	45.0	1
CGAACGC	30	2.166349E-6	44.999996	15
GTCGATA	30	2.166349E-6	44.999996	21
CACAACG	105	0.0	44.999996	12
CGTTTTT	7485	0.0	42.86573	1
CGACGGT	570	0.0	42.23684	28
CGGTCTA	575	0.0	41.478264	31
TCGACGG	125	0.0	41.399998	2
CGTTAGG	370	0.0	41.351353	2
>>END_MODULE