FastQCFastQC Report
Thu 26 May 2016
SRR1545182_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545182_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1181048
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52640.4457058476878163No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC39330.33300932730930494No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAATA351.2119744E-745.0000046
CTATGCG253.8908707E-545.0000041
GTACCTA253.8908707E-545.0000049
CGAACCT351.2119744E-745.00000437
CTCCGAT302.1655524E-645.00000429
CGTTCGA302.1655524E-645.00000414
CCGAGTA253.8908707E-545.00000439
TGTTACG351.2119744E-745.0000041
TTACGCG551.8189894E-1245.0000041
TACTACG253.8908707E-545.0000041
ACGTAAC502.1827873E-1145.00000413
TAGTGCG551.8189894E-1245.0000041
CGCAACA351.2119744E-745.00000430
TCGACAG253.8908707E-545.0000041
TCGAATC302.1655524E-645.00000432
TAGTACG351.2119744E-745.0000041
TCACGAC3350.045.025
TCGCACA207.033367E-445.040
GTACACG406.8157533E-945.01
CGAGTGT207.033367E-445.019