FastQCFastQC Report
Thu 26 May 2016
SRR1545181_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545181_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1401994
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73680.525537199160624No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC25080.17888806942112448No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG14970.10677649119753722No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTCG207.033817E-445.00000429
TCGATAG351.2121927E-745.0000041
ATCGTAA406.8175723E-945.00000414
CTACGTC207.033817E-445.00000422
TAGGCCG453.8562575E-1045.0000045
CCGTTAG207.033817E-445.00000427
ACGTTAG551.8189894E-1245.01
ACCTTCG253.8912454E-545.025
CGTTTTT54150.043.29641
TCACGAC4250.042.88235525
CGGTCTA4450.041.9662931
CGACGGT4350.041.8965528
ACGGTCT4350.041.8965530
ACGGGTA1350.041.6666645
CTCACGA4450.041.46067424
GACGGTC4450.040.95505529
AATCGTA451.9288564E-840.00000413
AACGGGA3700.039.5270274
GATTGCG1600.039.3750041
TATGTCG403.458954E-739.3750041
Produced by FastQC (version 0.11.5)