##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545181_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1401994
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.998355199808273	31.0	31.0	33.0	28.0	34.0
2	31.283553281968395	31.0	31.0	34.0	28.0	34.0
3	31.39373848960837	31.0	31.0	34.0	28.0	34.0
4	35.179086358429494	37.0	35.0	37.0	32.0	37.0
5	35.03664281016894	35.0	35.0	37.0	32.0	37.0
6	35.078654402229965	37.0	35.0	37.0	32.0	37.0
7	35.70997522100665	37.0	35.0	37.0	35.0	37.0
8	35.70782114616753	37.0	35.0	37.0	35.0	37.0
9	37.39554163569887	39.0	37.0	39.0	35.0	39.0
10	36.49135873620001	38.0	35.0	39.0	32.0	39.0
11	36.21670278189493	38.0	35.0	39.0	31.0	39.0
12	35.56421282830026	37.0	35.0	39.0	30.0	39.0
13	35.276638131118965	37.0	35.0	39.0	30.0	39.0
14	36.16950286520484	38.0	35.0	40.0	29.0	41.0
15	36.435939811439994	38.0	35.0	40.0	30.0	41.0
16	36.545052261279295	38.0	35.0	40.0	31.0	41.0
17	36.48312902908286	38.0	35.0	40.0	31.0	41.0
18	36.401137237391886	38.0	35.0	40.0	30.0	41.0
19	36.33577461815101	38.0	35.0	40.0	30.0	41.0
20	36.185412348412335	38.0	34.0	40.0	30.0	41.0
21	36.067687165565616	38.0	34.0	40.0	30.0	41.0
22	35.99705847528591	38.0	34.0	40.0	30.0	41.0
23	35.94757966153921	37.0	34.0	40.0	30.0	41.0
24	35.85436884893944	37.0	34.0	40.0	30.0	41.0
25	35.76920371984473	37.0	34.0	40.0	30.0	41.0
26	35.626417802073334	37.0	34.0	40.0	29.0	41.0
27	35.51698366754779	37.0	34.0	40.0	29.0	41.0
28	35.51593444765099	37.0	34.0	40.0	29.0	41.0
29	35.46369028683432	37.0	34.0	40.0	29.0	41.0
30	35.378588638753094	37.0	34.0	40.0	29.0	41.0
31	35.21829836646947	37.0	34.0	40.0	27.0	41.0
32	35.08275784347151	37.0	34.0	40.0	27.0	41.0
33	34.88783332881596	37.0	33.0	40.0	26.0	41.0
34	34.69757502528542	37.0	33.0	40.0	25.0	41.0
35	34.53814566966763	37.0	33.0	40.0	24.0	41.0
36	34.38201376040126	36.0	33.0	40.0	24.0	41.0
37	34.321757439760795	36.0	33.0	40.0	23.0	41.0
38	34.24821718209921	36.0	33.0	40.0	23.0	41.0
39	34.19097870604296	36.0	33.0	40.0	23.0	41.0
40	34.04498378737712	36.0	33.0	40.0	23.0	41.0
41	33.971064070174336	36.0	33.0	40.0	23.0	41.0
42	33.90733412553834	36.0	33.0	40.0	23.0	41.0
43	33.8436070339816	35.0	33.0	40.0	23.0	41.0
44	33.73140398603703	35.0	32.0	40.0	23.0	41.0
45	33.692677714740576	35.0	32.0	40.0	23.0	41.0
46	33.64391431061759	35.0	32.0	39.0	23.0	41.0
47	33.520164137649665	35.0	32.0	39.0	23.0	41.0
48	33.46824808094757	35.0	32.0	39.0	23.0	41.0
49	33.42669797445638	35.0	32.0	39.0	23.0	41.0
50	33.34851932319254	35.0	32.0	39.0	23.0	41.0
51	33.20252012490781	35.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	1.0
12	0.0
13	2.0
14	11.0
15	60.0
16	180.0
17	490.0
18	1050.0
19	2050.0
20	3349.0
21	5456.0
22	8074.0
23	11463.0
24	15695.0
25	20741.0
26	26606.0
27	30698.0
28	34503.0
29	39383.0
30	46116.0
31	55575.0
32	67584.0
33	84601.0
34	126189.0
35	161632.0
36	112449.0
37	135958.0
38	176470.0
39	235489.0
40	117.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.01733102994734	23.587476123292966	29.588500378746275	13.806692468013415
2	30.842999328099836	25.581849850997934	30.765894861176296	12.809255959725933
3	28.945487641173926	25.63612968386455	31.728024513656976	13.690358161304541
4	26.102964777310035	27.678435143089057	31.597781445569666	14.620818634031243
5	23.414151558423217	32.10498761050333	30.643569088027483	13.837291743045977
6	20.07383769117414	41.72820996380869	27.866381739151524	10.331570605865647
7	87.61642346543566	3.854224768436955	6.963296561896841	1.5660552042305458
8	88.80137860789704	3.248587369132821	6.325704674913017	1.6243293480571246
9	84.49465546928161	4.724128633931386	8.052887530189144	2.72832836659786
10	44.05453946307901	26.67857351743303	15.690152739598028	13.57673427988993
11	39.55230906836976	23.078486783823614	21.793531213400342	15.575672934406281
12	35.71555941038264	21.279834293156746	25.7702957359304	17.234310560530215
13	24.45595344915884	31.391004526410242	25.970011283928464	18.183030740502456
14	19.601867055065856	33.53166989302379	28.87651445013317	17.989948601777183
15	18.059278427725083	23.528631363614966	40.231983874396036	18.18010633426391
16	20.82904777053254	19.63068315556272	37.98061903260642	21.559650041298323
17	21.286325048466683	20.564567323397963	28.72644248120891	29.422665146926448
18	23.76878931008264	22.637115422747886	32.53972556230626	21.054369704863216
19	28.718025897400416	24.66993439344248	25.3006075632278	21.3114321459293
20	29.630725951751575	24.28006111295769	25.437769348513616	20.65144358677712
21	24.035409566660057	26.70803156076274	28.563674309590482	20.69288456298672
22	23.911871234826968	22.780197347492216	27.644126865022244	25.66380455265857
23	21.631618965559056	28.02465631093999	26.45596200839661	23.887762715104344
24	21.153656863010823	24.9396217102213	34.41847825311663	19.48824317365124
25	21.276267944085355	24.856240468932107	31.542645688925912	22.324845898056626
26	19.66698858910951	29.72523420214352	27.22643606178058	23.381341146966392
27	19.788315784518336	29.540639974208165	29.15504631260904	21.51599792866446
28	18.588381975957102	27.20853298944218	33.66669186886677	20.536393165733948
29	19.673194036493737	25.500109130281583	32.54200802571195	22.28468880751273
30	22.469639670355225	27.791702389596534	29.96589143748119	19.77276650256706
31	27.659248185084955	24.497608406312725	26.788131760906253	21.055011647696066
32	28.366241224998113	25.63784153141882	26.281567538805444	19.71434970477762
33	27.413384080103054	25.823220356149886	25.60595837072056	21.157437193026503
34	21.42548398923248	26.81010047118604	28.032288297952775	23.73212724162871
35	22.262220808362944	25.37821131902134	29.603479044846125	22.75608882776959
36	28.252688670564925	25.562520239030977	25.6961156752454	20.488675415158696
37	21.374913159400112	31.17759419797802	26.67479318741735	20.772699455204517
38	22.985833034948794	29.326516375961663	24.961590420501086	22.726060168588454
39	22.290537619989813	29.2902822693963	25.091619507644115	23.327560602969772
40	24.62756616647432	25.787770846380226	25.762592421936187	23.822070565209266
41	21.22762294275154	24.524213370385322	26.509671225411807	27.73849246145133
42	22.11771234399006	26.412809184632746	24.521360291128207	26.948118180248986
43	21.993746050268403	26.22714505197597	26.43278073943255	25.34632815832307
44	21.398451063271313	27.190201955215215	28.203972342249685	23.207374639263794
45	19.725548040861803	32.08023714794785	24.86387245594489	23.330342355245456
46	21.977768806428557	29.066743509601324	27.00653497803842	21.948952705931696
47	21.839893751328464	27.600688733332667	26.899473178915173	23.659944336423695
48	22.27427506822426	26.391339763222955	28.80404623700244	22.530338931550347
49	22.966646076944695	25.262162320238176	29.50547577236422	22.265715830452912
50	21.254085252861284	29.924022499383025	26.285347868821123	22.536544378934575
51	20.338032830383014	30.66175746829159	25.856244748550992	23.143964952774407
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	724.0
1	1216.0
2	1708.0
3	7040.0
4	12372.0
5	8959.0
6	5546.0
7	5753.0
8	5960.0
9	6280.0
10	6600.0
11	6708.0
12	6816.0
13	6761.5
14	6707.0
15	6471.0
16	6235.0
17	5738.0
18	5241.0
19	5004.0
20	4767.0
21	4863.0
22	4959.0
23	5207.5
24	5456.0
25	5816.0
26	8297.0
27	10418.0
28	12383.5
29	14349.0
30	16211.0
31	18073.0
32	23328.5
33	28584.0
34	31921.5
35	35259.0
36	36907.5
37	38556.0
38	46430.5
39	54305.0
40	66786.5
41	79268.0
42	91145.5
43	103023.0
44	107889.0
45	112755.0
46	111297.0
47	109839.0
48	109342.0
49	108845.0
50	105455.0
51	102065.0
52	93628.5
53	85192.0
54	82696.5
55	80201.0
56	77891.0
57	75581.0
58	70424.0
59	65267.0
60	61109.0
61	56951.0
62	49088.5
63	41226.0
64	37552.5
65	33879.0
66	29270.0
67	24661.0
68	20380.0
69	16099.0
70	12856.5
71	9614.0
72	8807.0
73	8000.0
74	6519.5
75	3934.0
76	2829.0
77	2030.0
78	1231.0
79	1068.0
80	905.0
81	621.5
82	338.0
83	308.5
84	279.0
85	169.0
86	59.0
87	42.5
88	26.0
89	17.0
90	8.0
91	5.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1401994.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.996826931654216
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.71258569722427	16.317177122959645
2	7.265069075985446	3.050867960699588
3	2.885437620276492	1.8175510300508908
4	1.6596253474283063	1.3938746476535457
5	1.0739928026545704	1.1275220501590137
6	0.7527182635163144	0.9482817064408406
7	0.5902455141241616	0.86752980350752
8	0.49207809423328747	0.8265662865179655
9	0.40928728837495953	0.7734360923401568
>10	4.9799841001406415	24.100642683155428
>50	1.2678918366261422	19.114512636351602
>100	0.9063178493138797	28.319014560470603
>500	0.0037451150798091066	0.5300034935649923
>1k	6.809300145107466E-4	0.28630482758670006
>5k	3.404650072553733E-4	0.5267150985415245
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7368	0.525537199160624	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2508	0.17888806942112448	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1497	0.10677649119753722	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02703292596116674	0.0
2	0.0	0.0	0.0	0.10691914516039298	0.0
3	0.0	0.0	0.0	0.1527823942185202	0.0
4	0.0	0.0	0.0	0.21612075372647815	0.0
5	0.0	0.0	0.0	0.36590741472502736	0.0
6	0.0	0.0	0.0	0.5243246404763501	0.0
7	0.0	0.0	0.0	0.6305305158224643	0.0
8	0.0	0.0	0.0	0.8871649949999786	0.0
9	0.0	0.0	0.0	1.0084208634273757	0.0
10	0.0	0.0	0.0	1.2235430394138633	0.0
11	0.0	0.0	0.0	1.4022884548721322	0.0
12	0.0	0.0	0.0	1.5763262895561607	0.0
13	0.0	0.0	0.0	1.6450854996526376	0.0
14	0.0	0.0	0.0	1.677111314313756	0.0
15	0.0	0.0	0.0	1.7192655603376334	0.0
16	0.0	0.0	0.0	1.812061963175306	0.0
17	0.0	0.0	0.0	1.9199796860756893	0.0
18	0.0	0.0	0.0	2.0887393241340546	0.0
19	0.0	0.0	0.0	2.157355880267676	0.0
20	0.0	0.0	0.0	2.232962480581229	0.0
21	0.0	0.0	0.0	2.3296105404160077	0.0
22	0.0	0.0	0.0	2.4292543334707566	0.0
23	0.0	0.0	0.0	2.5408810594053897	0.0
24	0.0	0.0	0.0	2.620909932567472	0.0
25	0.0	0.0	0.0	2.6929501838096312	0.0
26	0.0	0.0	0.0	2.7678435143089057	0.0
27	0.0	0.0	0.0	2.850796793709531	0.0
28	7.132698142788058E-5	0.0	0.0	2.935675901608709	0.0
29	7.132698142788058E-5	0.0	0.0	3.037031542217727	0.0
30	1.4265396285576115E-4	0.0	0.0	3.1663473595464744	0.0
31	1.4265396285576115E-4	0.0	0.0	3.269200866765478	0.0
32	1.4265396285576115E-4	0.0037803300156776707	0.0	3.3676321011359533	0.0
33	1.4265396285576115E-4	0.0037803300156776707	0.0	3.462496986435035	0.0
34	2.1398094428364174E-4	0.0037803300156776707	0.0	3.574694328221091	0.0
35	2.1398094428364174E-4	0.0037803300156776707	0.0	3.701228393274151	0.0
36	2.1398094428364174E-4	0.0037803300156776707	0.0	3.8106439827845198	0.0
37	2.1398094428364174E-4	0.0037803300156776707	0.0	3.925765730809119	0.0
38	2.1398094428364174E-4	0.0037803300156776707	0.0	4.062285573262082	0.0
39	2.1398094428364174E-4	0.0037803300156776707	0.0	4.28482575531707	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCGTCG	20	7.033817E-4	45.000004	29
TCGATAG	35	1.2121927E-7	45.000004	1
ATCGTAA	40	6.8175723E-9	45.000004	14
CTACGTC	20	7.033817E-4	45.000004	22
TAGGCCG	45	3.8562575E-10	45.000004	5
CCGTTAG	20	7.033817E-4	45.000004	27
ACGTTAG	55	1.8189894E-12	45.0	1
ACCTTCG	25	3.8912454E-5	45.0	25
CGTTTTT	5415	0.0	43.2964	1
TCACGAC	425	0.0	42.882355	25
CGGTCTA	445	0.0	41.96629	31
CGACGGT	435	0.0	41.89655	28
ACGGTCT	435	0.0	41.89655	30
ACGGGTA	135	0.0	41.666664	5
CTCACGA	445	0.0	41.460674	24
GACGGTC	445	0.0	40.955055	29
AATCGTA	45	1.9288564E-8	40.000004	13
AACGGGA	370	0.0	39.527027	4
GATTGCG	160	0.0	39.375004	1
TATGTCG	40	3.458954E-7	39.375004	1
>>END_MODULE