FastQCFastQC Report
Thu 26 May 2016
SRR1545180_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545180_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2784472
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164240.5898425266980598No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAA453.8562575E-1045.027
CGACGGT7950.043.30188428
CGTTTTT111200.043.1991921
CGGTCTA8100.043.05555731
TAGTACG2150.042.9069751
ACGGTCT8350.041.22754730
TCACGAC8500.041.0294125
ACGGGTA3450.040.434785
AGTACGG4850.040.3608252
TAGCGAT451.9299478E-840.045
TACGGGA10700.039.532714
GACGGTC9150.039.3442629
CTCACGA9000.039.00000424
ACGGGAG12250.038.9387785
CACGACG8900.038.93258326
CGGGTAT5000.038.76
TACGGGT2100.038.571434
CGCATCG1400.038.57142621
CGCGTAG356.2514937E-638.5714262
GGGCGAT45100.038.5643047
Produced by FastQC (version 0.11.5)