##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545180_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2784472
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.039033971252	31.0	31.0	33.0	30.0	34.0
2	31.319791328481667	31.0	31.0	34.0	30.0	34.0
3	31.429521288057483	31.0	31.0	34.0	30.0	34.0
4	35.209883956455656	37.0	35.0	37.0	32.0	37.0
5	35.06210584987028	37.0	35.0	37.0	32.0	37.0
6	35.11355869263544	37.0	35.0	37.0	32.0	37.0
7	35.7426571357155	37.0	35.0	37.0	35.0	37.0
8	35.745997445835336	37.0	35.0	37.0	35.0	37.0
9	37.43011278260295	39.0	37.0	39.0	35.0	39.0
10	36.561653340381945	39.0	35.0	39.0	32.0	39.0
11	36.339162325927504	38.0	35.0	39.0	32.0	39.0
12	35.83557564953068	37.0	35.0	39.0	31.0	39.0
13	35.63674944477804	37.0	35.0	39.0	30.0	39.0
14	36.59395174381355	38.0	35.0	40.0	31.0	41.0
15	36.79416241211978	38.0	35.0	41.0	31.0	41.0
16	36.856392881666615	38.0	35.0	41.0	31.0	41.0
17	36.7759280754125	38.0	35.0	40.0	31.0	41.0
18	36.680601564677254	38.0	35.0	40.0	31.0	41.0
19	36.63935101520145	38.0	35.0	40.0	31.0	41.0
20	36.52744326392939	38.0	35.0	40.0	31.0	41.0
21	36.41237261498769	38.0	35.0	40.0	30.0	41.0
22	36.3418105838378	38.0	35.0	40.0	30.0	41.0
23	36.263551581772056	38.0	35.0	40.0	30.0	41.0
24	36.19716879896799	38.0	34.0	40.0	30.0	41.0
25	36.0876123013627	38.0	34.0	40.0	30.0	41.0
26	35.96025997029239	38.0	34.0	40.0	30.0	41.0
27	35.86553644640708	38.0	34.0	40.0	30.0	41.0
28	35.84817947531884	38.0	34.0	40.0	29.0	41.0
29	35.79801125671222	38.0	34.0	40.0	29.0	41.0
30	35.696416053025494	38.0	34.0	40.0	29.0	41.0
31	35.5824217302239	38.0	34.0	40.0	29.0	41.0
32	35.45475695212593	38.0	34.0	40.0	28.0	41.0
33	35.26416103304325	38.0	34.0	40.0	27.0	41.0
34	35.06644168086445	38.0	34.0	40.0	26.0	41.0
35	34.92420466070408	37.0	33.0	40.0	25.0	41.0
36	34.77822186755694	37.0	33.0	40.0	25.0	41.0
37	34.726646919056826	37.0	33.0	40.0	24.0	41.0
38	34.667209079495144	37.0	33.0	40.0	24.0	41.0
39	34.593698194846276	37.0	33.0	40.0	24.0	41.0
40	34.48167049264636	37.0	33.0	40.0	24.0	41.0
41	34.40398287359327	37.0	33.0	40.0	24.0	41.0
42	34.32633152712615	37.0	33.0	40.0	23.0	41.0
43	34.28295346478614	37.0	33.0	40.0	23.0	41.0
44	34.16608822067523	36.0	33.0	40.0	23.0	41.0
45	34.09456334989183	36.0	33.0	40.0	23.0	41.0
46	34.02547089717548	36.0	33.0	40.0	23.0	41.0
47	33.92665431722783	36.0	33.0	40.0	23.0	41.0
48	33.84802468834307	36.0	33.0	40.0	23.0	41.0
49	33.794559614892876	36.0	32.0	40.0	23.0	41.0
50	33.74722101712641	35.0	32.0	40.0	23.0	41.0
51	33.600059544502514	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	0.0
11	2.0
12	5.0
13	10.0
14	29.0
15	101.0
16	323.0
17	787.0
18	1755.0
19	3475.0
20	5706.0
21	8992.0
22	13468.0
23	19244.0
24	26864.0
25	37433.0
26	48203.0
27	56996.0
28	63666.0
29	73295.0
30	86999.0
31	104870.0
32	127561.0
33	159048.0
34	230438.0
35	295048.0
36	231297.0
37	282788.0
38	375554.0
39	530240.0
40	273.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.50947684156996	23.51106421612428	29.045758046767933	13.933700895537825
2	31.380994314182363	25.69144168086445	29.936842604271114	12.990721400682068
3	28.46582763267147	25.81875486627267	32.49452678999825	13.22089071105761
4	26.068927969108685	27.65429137014127	31.91179512668829	14.364985534061752
5	22.797751243323688	31.809298136235526	30.858633162768378	14.534317457672405
6	19.960552664921753	42.08094030035138	27.590724561065795	10.367782473661075
7	87.50430961417462	3.974757153241261	6.881376433305847	1.6395567992782831
8	88.65705957897943	2.921056487549525	6.660400966502805	1.761482966968244
9	83.88075010271248	5.437476117554783	7.9472158455893975	2.734557934143349
10	37.726434311424214	34.49548065126889	14.816740839915072	12.961344197391822
11	34.394671593034516	24.437379869504884	24.714559887835108	16.453388649625495
12	32.2607661344772	22.385141599556395	27.890027265492346	17.464065000474058
13	24.07400756768249	29.723947664045465	28.247904809242115	17.954139959029934
14	19.22709224585487	32.7772015664011	29.71773463694374	18.277971550800295
15	17.88568892055657	24.73829867924691	38.54105913077955	18.834953269416967
16	20.942749648766444	22.120423548881078	35.837602245596294	21.099224556756184
17	21.032569190855575	21.647694787378	29.676649648479135	27.64308637328729
18	22.30893325556874	24.109813278783196	32.59386339672297	20.987390068925098
19	25.69704417929144	26.237936671656243	26.530308079951965	21.534711069100354
20	27.168310545051273	25.56987464768904	26.90071941825955	20.36109538900014
21	24.33725316684815	26.808745069083116	29.068132127024445	19.785869637044296
22	23.796324761031894	23.75322861928581	28.149286471546493	24.301160148135807
23	20.801178823130563	27.495158866743857	27.60681378731767	24.096848522807914
24	20.24965594913506	25.832222410568324	34.28919378611098	19.62892785418564
25	20.89121384592842	26.71946422876581	30.929490402489236	21.459831522816533
26	19.957823242611166	29.369733292344115	27.827286465800338	22.84515699924438
27	19.502045630194882	29.01102973921088	29.269355195527197	22.217569435067045
28	19.402565369664337	26.90348475402159	32.633080885711905	21.06086899060217
29	19.751033589132877	25.05200267770694	32.433330268718805	22.763633464441373
30	21.68798967991059	27.23115908509764	31.102090450182295	19.97876078480947
31	25.655635969763747	26.842970588319798	27.189535394861213	20.31185804705524
32	26.16567880732864	25.879915474100656	27.37452558330628	20.57988013526442
33	25.43591747376163	26.32951597286667	26.838337753082094	21.396228800289606
34	20.661655064227617	27.094831623374198	29.139635808871482	23.1038775035267
35	21.37442933525638	26.65514323720978	29.35838464168431	22.612042785849525
36	25.709613887300716	27.286860848304457	26.172646016910928	20.830879247483907
37	21.125728683930024	30.277302124065177	27.219666780632018	21.377302411372785
38	22.056892653257062	29.923446886878374	26.067706911759213	21.951953548105347
39	20.971516323381955	29.773256832893274	26.382631967568713	22.872594876156054
40	23.03237382167966	26.410033931028938	27.413419851232117	23.14417239605929
41	20.771191091165576	25.755942239677754	27.764832973719972	25.708033695436693
42	21.069344565145563	27.236689756621722	26.312313429619692	25.381652248613022
43	20.976328725876936	26.718207257964888	27.711645152115018	24.593818864043165
44	21.48281613174778	27.54511447771786	28.82086801375629	22.151201376778076
45	19.82677505825162	30.586050066224406	26.21046288129311	23.376711994230863
46	21.615336767616984	28.801295182713275	28.63106542281625	20.952302626853495
47	21.070350141786307	28.81663022648459	27.185441261395336	22.927578370333766
48	21.285292148744897	27.39373927983474	29.02916603219569	22.291802539224673
49	21.68770237229895	26.055532251715945	29.805076150882464	22.45168922510264
50	21.207000824572845	28.348713867476494	28.3735659758834	22.070719332067267
51	19.790430645379086	29.16991084844811	27.8032603667769	23.236398139395906
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1174.0
1	2162.5
2	3151.0
3	15314.0
4	27477.0
5	19673.0
6	11869.0
7	12254.5
8	12640.0
9	13254.0
10	13868.0
11	14059.0
12	14250.0
13	13920.0
14	13590.0
15	12922.5
16	12255.0
17	11763.0
18	11271.0
19	11027.0
20	10783.0
21	11492.5
22	12202.0
23	14242.0
24	16282.0
25	17010.0
26	20485.5
27	23233.0
28	32491.5
29	41750.0
30	46560.5
31	51371.0
32	60112.0
33	68853.0
34	79774.5
35	90696.0
36	96466.0
37	102236.0
38	116986.0
39	131736.0
40	147009.5
41	162283.0
42	182233.5
43	202184.0
44	202257.5
45	202331.0
46	205280.0
47	208229.0
48	208843.5
49	209458.0
50	199599.0
51	189740.0
52	179018.0
53	168296.0
54	157110.0
55	145924.0
56	138287.0
57	130650.0
58	124859.5
59	119069.0
60	107285.0
61	95501.0
62	86266.0
63	77031.0
64	69214.5
65	61398.0
66	51662.5
67	41927.0
68	34588.5
69	27250.0
70	23710.5
71	20171.0
72	17599.0
73	15027.0
74	11945.5
75	7139.0
76	5414.0
77	4059.0
78	2704.0
79	2026.0
80	1348.0
81	1074.0
82	800.0
83	549.5
84	299.0
85	187.5
86	76.0
87	64.5
88	53.0
89	30.5
90	8.0
91	5.0
92	2.0
93	4.5
94	7.0
95	4.5
96	2.0
97	1.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2784472.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.581829318911357
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.6381377326146	14.42658623985576
2	7.525814317504263	2.7968679426736713
3	2.9412930366827648	1.6396381555362483
4	1.6503275777927318	1.2266442148334777
5	1.089232719326766	1.0119968239551822
6	0.7955710462005352	0.8869899234939651
7	0.5944888017932964	0.773268261284901
8	0.5012555904517155	0.7451396661539128
9	0.38549157598465755	0.6446824801942549
>10	4.4634116458295185	19.192097961537854
>50	1.2319611965453798	16.298164610141708
>100	1.1746535519897183	38.43383574885449
>500	0.006027847111828004	0.7456509326884597
>1k	0.002138913491293808	0.5850091704782115
>5k	0.0	0.0
>10k+	1.9444668102670977E-4	0.5934278683178399
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16424	0.5898425266980598	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.027545617266038228	0.0
2	0.0	0.0	0.0	0.09743319379760328	0.0
3	0.0	0.0	0.0	0.14128351802424302	0.0
4	0.0	0.0	0.0	0.20219273169204072	0.0
5	0.0	0.0	0.0	0.3496174499151006	0.0
6	0.0	0.0	0.0	0.5430113860006494	0.0
7	0.0	0.0	0.0	0.6793747611755478	0.0
8	0.0	0.0	0.0	1.0329786042021611	0.0
9	0.0	0.0	0.0	1.2038548062253813	0.0
10	0.0	0.0	0.0	1.471805067531654	0.0
11	0.0	0.0	0.0	1.6609253028940496	0.0
12	0.0	0.0	0.0	1.8390560221111938	0.0
13	0.0	0.0	0.0	1.914151049103744	0.0
14	0.0	0.0	0.0	1.9439592138114514	0.0
15	0.0	0.0	0.0	1.9847209812129554	0.0
16	7.18269029101388E-5	0.0	0.0	2.0712364857682175	0.0
17	7.18269029101388E-5	0.0	0.0	2.1753495815364636	0.0
18	7.18269029101388E-5	0.0	0.0	2.3354876615746183	0.0
19	7.18269029101388E-5	0.0	0.0	2.399629085873372	0.0
20	7.18269029101388E-5	0.0	0.0	2.4759092567639396	0.0
21	7.18269029101388E-5	0.0	0.0	2.563645818668674	0.0
22	7.18269029101388E-5	0.0	0.0	2.658816465024608	0.0
23	7.18269029101388E-5	0.0	0.0	2.7671673480645524	0.0
24	7.18269029101388E-5	0.0	0.0	2.8532518912023535	0.0
25	7.18269029101388E-5	0.0	0.0	2.9308249463453033	0.0
26	7.18269029101388E-5	0.0	0.0	3.0025800223525323	0.0
27	7.18269029101388E-5	0.0	0.0	3.0834571150293484	0.0
28	7.18269029101388E-5	0.0	0.0	3.1636518521285186	0.0
29	7.18269029101388E-5	0.0	0.0	3.2620906225668636	0.0
30	1.0774035436520819E-4	0.0	0.0	3.3919177495769395	0.0
31	1.0774035436520819E-4	0.0	0.0	3.488884068505627	0.0
32	1.0774035436520819E-4	0.0	0.0	3.5838033207013753	0.0
33	1.0774035436520819E-4	0.0	0.0	3.686228484251233	0.0
34	1.0774035436520819E-4	0.0	0.0	3.796662347475572	0.0
35	1.0774035436520819E-4	0.0	0.0	3.929218896796233	0.0
36	1.0774035436520819E-4	0.0	0.0	4.040442855952583	0.0
37	1.0774035436520819E-4	0.0	0.0	4.154863112288434	0.0
38	1.0774035436520819E-4	3.59134514550694E-5	0.0	4.284259277881049	0.0
39	1.436538058202776E-4	3.59134514550694E-5	0.0	4.463251919933115	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGTAA	45	3.8562575E-10	45.0	27
CGACGGT	795	0.0	43.301884	28
CGTTTTT	11120	0.0	43.199192	1
CGGTCTA	810	0.0	43.055557	31
TAGTACG	215	0.0	42.906975	1
ACGGTCT	835	0.0	41.227547	30
TCACGAC	850	0.0	41.02941	25
ACGGGTA	345	0.0	40.43478	5
AGTACGG	485	0.0	40.360825	2
TAGCGAT	45	1.9299478E-8	40.0	45
TACGGGA	1070	0.0	39.53271	4
GACGGTC	915	0.0	39.34426	29
CTCACGA	900	0.0	39.000004	24
ACGGGAG	1225	0.0	38.938778	5
CACGACG	890	0.0	38.932583	26
CGGGTAT	500	0.0	38.7	6
TACGGGT	210	0.0	38.57143	4
CGCATCG	140	0.0	38.571426	21
CGCGTAG	35	6.2514937E-6	38.571426	2
GGGCGAT	4510	0.0	38.564304	7
>>END_MODULE