FastQCFastQC Report
Thu 26 May 2016
SRR1545179_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545179_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2124912
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT428912.018483588967449No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT269750.044.132531
CGGTCTA8550.042.10526331
TCACGAC8650.041.35838325
CGACGGT9000.040.7528
GCGTAAG1550.040.645161
ACGGTCT9150.039.83606730
GTTTTTT308400.039.2509732
CACGACG9150.039.09836226
TACGGGA8100.038.8888854
TCTCACG9250.038.67567423
GACGGTC9400.038.53723529
GGGCGAT33600.038.5044637
AGGGCGA18650.038.243976
TAGGGCG9150.038.1147545
TAGGGTA16200.038.0555535
ATACTAT25800.037.93604745
TAAGGGA25550.037.866934
CGTTCGA1550.037.74193614
TATGGGC8850.037.6271174
TAGGGAC19750.037.5949365
Produced by FastQC (version 0.11.5)