FastQCFastQC Report
Thu 26 May 2016
SRR1545178_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545178_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1751013
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63840.3645889550791456No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC53290.3043381174211728No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT24750.14134675185164244No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCCGC302.1661563E-645.00000426
TAAGTCG302.1661563E-645.00000415
CTAACCG302.1661563E-645.00000426
CTTATCG302.1661563E-645.00000421
CGTAGCA302.1661563E-645.00000440
ACGTACT600.045.00000419
TAGGCCG302.1661563E-645.0000045
TATCGAC302.1661563E-645.00000423
TGCACGA302.1661563E-645.0000042
AGTCGAT302.1661563E-645.00000426
CTAGACG351.212411E-745.01
CTATGCG351.212411E-745.01
TCGTTAC207.0343E-445.039
ACCGGTA502.1827873E-1145.041
ACACGTC207.0343E-445.029
GTCGATC253.891646E-545.044
TAGCGAT207.0343E-445.018
CCGTGTA351.212411E-745.011
ATAGCCG253.891646E-545.01
CGCGATA207.0343E-445.011
Produced by FastQC (version 0.11.5)