##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545178_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1751013
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.07222105147135	31.0	31.0	33.0	30.0	34.0
2	31.353111598828793	31.0	31.0	34.0	30.0	34.0
3	31.46063849897174	31.0	31.0	34.0	30.0	34.0
4	35.25828591792294	37.0	35.0	37.0	33.0	37.0
5	35.08084805766719	37.0	35.0	37.0	32.0	37.0
6	35.14331703990776	37.0	35.0	37.0	32.0	37.0
7	35.73755134884778	37.0	35.0	37.0	35.0	37.0
8	35.7425518828244	37.0	35.0	37.0	35.0	37.0
9	37.44158153023421	39.0	37.0	39.0	35.0	39.0
10	36.61622729243015	39.0	35.0	39.0	32.0	39.0
11	36.29506063061782	38.0	35.0	39.0	32.0	39.0
12	35.49855083885728	37.0	35.0	39.0	30.0	39.0
13	35.106191673048684	37.0	34.0	39.0	30.0	39.0
14	36.046844883504576	38.0	35.0	40.0	30.0	41.0
15	36.40108097427032	38.0	35.0	40.0	31.0	41.0
16	36.56108778175833	38.0	35.0	40.0	31.0	41.0
17	36.48266460614513	38.0	35.0	40.0	31.0	41.0
18	36.43320066727089	38.0	35.0	40.0	31.0	41.0
19	36.350466272951714	37.0	35.0	40.0	31.0	41.0
20	36.13554154081095	37.0	34.0	40.0	30.0	41.0
21	36.041107062026384	37.0	34.0	40.0	30.0	41.0
22	35.94249728585682	37.0	34.0	40.0	30.0	41.0
23	35.85515527297627	37.0	34.0	40.0	30.0	41.0
24	35.82471346586233	37.0	34.0	40.0	30.0	41.0
25	35.771663602725965	37.0	34.0	40.0	30.0	41.0
26	35.595919619100485	37.0	34.0	40.0	29.0	41.0
27	35.48900379380393	36.0	34.0	40.0	29.0	41.0
28	35.53992860132963	36.0	34.0	40.0	29.0	41.0
29	35.5265015165507	36.0	34.0	40.0	29.0	41.0
30	35.48516887081935	36.0	34.0	40.0	29.0	41.0
31	35.34202144701381	36.0	34.0	40.0	29.0	41.0
32	35.10771878906667	36.0	34.0	40.0	27.0	41.0
33	34.87173024986108	36.0	33.0	40.0	26.0	41.0
34	34.70160187274452	36.0	34.0	40.0	26.0	41.0
35	34.53233928017667	36.0	33.0	40.0	25.0	41.0
36	34.324841677360475	36.0	33.0	40.0	24.0	41.0
37	34.245084987946974	36.0	33.0	40.0	24.0	41.0
38	34.26586553041011	36.0	33.0	40.0	24.0	41.0
39	34.214396466502535	36.0	33.0	40.0	24.0	41.0
40	34.05463009126717	36.0	33.0	40.0	23.0	41.0
41	34.07633352807775	36.0	33.0	40.0	23.0	41.0
42	33.95047381144515	35.0	33.0	40.0	23.0	41.0
43	33.85804559988989	35.0	33.0	40.0	23.0	41.0
44	33.766920062843624	35.0	33.0	40.0	23.0	41.0
45	33.724601701986224	35.0	33.0	40.0	23.0	41.0
46	33.638674869918155	35.0	33.0	39.0	23.0	41.0
47	33.521964714139756	35.0	32.0	39.0	23.0	41.0
48	33.53208571267032	35.0	33.0	39.0	23.0	41.0
49	33.49495063714547	35.0	32.0	39.0	23.0	41.0
50	33.400929062205705	35.0	32.0	39.0	23.0	41.0
51	33.25966683285618	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	7.0
14	15.0
15	76.0
16	166.0
17	466.0
18	1157.0
19	2248.0
20	4030.0
21	6477.0
22	10349.0
23	15049.0
24	20955.0
25	27000.0
26	31938.0
27	35945.0
28	39312.0
29	45888.0
30	55233.0
31	66725.0
32	83254.0
33	106621.0
34	166499.0
35	237020.0
36	129538.0
37	155146.0
38	204982.0
39	304688.0
40	226.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.09986847613353	24.364410772507114	29.196813501670178	13.33890724968918
2	29.197898587846005	26.9800395542466	31.404392771498557	12.41766908640884
3	26.498261292177727	27.30705026176276	33.01032031172812	13.184368134331384
4	25.44030227074271	28.209899069852707	31.982686593417636	14.367112065986944
5	23.755049220080036	32.06463915459223	30.269221302183364	13.911090323144373
6	19.485691996575696	42.80533611115394	27.04228923486005	10.66668265741031
7	86.51991732785537	4.659874027205966	7.387952002640757	1.4322566422979155
8	87.92738831750535	4.847536825825965	5.478600101769661	1.7464747548990214
9	83.91114172196323	5.424345792978122	7.059285110961483	3.6052273740971654
10	46.76904169186636	29.036277857445945	12.921091962195597	11.27358848849209
11	41.91750718012944	22.065570044311492	22.451803613108527	13.565119162450538
12	38.84357226359827	21.232794959260726	24.929512230920047	14.99412054622096
13	21.92416618266112	39.32814890580481	24.275033937497895	14.472650974036172
14	15.054771152470028	41.8047724374405	27.262333289358786	15.878123120730686
15	12.93434143550048	24.149278160698977	44.9447833910999	17.97159701270065
16	15.056541556230593	20.391167855407126	42.76387439727746	21.788416191084817
17	15.466989679688274	21.827022415024903	29.430906566655985	33.27508133863084
18	19.82292535806416	24.172693178177433	33.866167755464986	22.138213708293428
19	26.954968352605036	26.159942844513434	25.181651992303884	21.70343681057765
20	29.752663172689182	24.19319559592076	25.35189630231186	20.702244929078194
21	20.51395392267219	30.769446029241358	27.61121704978775	21.1053829982987
22	21.072944632621233	26.283242899967046	25.511403970158987	27.132408497252737
23	19.279582732966574	31.546767499727302	24.75447069781892	24.419179069487203
24	18.051150962328663	25.339960354377723	38.97880826698603	17.63008041630759
25	17.05224347277833	28.476716049509626	33.77433519911045	20.696705278601588
26	16.50421784418505	34.607510052752325	27.244515032155668	21.643757070906954
27	16.828201732368633	35.0782661236667	26.89614525991526	21.197386884049404
28	14.925246128955067	30.205086998211893	35.71555436767174	19.154112505161297
29	15.477497882654212	24.724145394694386	36.854723522897885	22.943633199753513
30	18.075194187593123	32.60695380331271	30.01936593274864	19.298486076345522
31	26.7189906642612	28.390194704436805	24.895931669268016	19.994882962033977
32	29.51000363789418	27.083636729139076	24.873259079173028	18.53310055379372
33	28.209442191462884	27.81812585057907	23.657905452443813	20.314526505514234
34	19.33395126135557	28.079974277746654	26.782325431050484	25.803749029847296
35	20.682085170127234	26.678899585554188	30.41068227363246	22.22833297068611
36	28.378772744691215	27.49402774279803	24.760981214873905	19.366218297636852
37	19.100257964960853	33.50306365515276	27.888428012813154	19.50825036707323
38	19.730693033118545	32.40558465299801	25.447440995583698	22.41628131829975
39	19.5421164777189	33.096327668612396	25.955832423859786	21.405723429808916
40	23.326383070828143	27.44576996287292	25.668170367667177	23.55967659863176
41	17.394388276957397	25.483877047172122	27.59088596144061	29.530848714429876
42	20.60361630667505	25.626937092985603	25.46674410755374	28.302702492785603
43	21.106125425682162	26.550231209020147	26.108486915859565	26.235156449438122
44	19.426240696099914	29.33621852036507	29.129309719573754	22.108231063961263
45	17.015293433001354	36.9148601409584	23.0926326646347	22.97721376140554
46	20.701673831090915	33.91242669243461	25.75743298307894	19.62846649339554
47	20.406701720661125	28.941646920953758	26.774786937618394	23.876864420766722
48	20.788652054553562	26.747488453826442	31.035634801112273	21.428224690507726
49	20.38505710694324	25.688729895209228	32.020778829169174	21.905434168678358
50	19.28420862666354	32.81357705511038	27.26376103432699	20.638453283899093
51	18.203291466139888	32.15978407927297	26.229159920571693	23.407764534015453
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1606.0
1	2170.0
2	2734.0
3	6908.5
4	11083.0
5	8458.5
6	5834.0
7	6664.5
8	7495.0
9	8172.5
10	8850.0
11	9326.0
12	9802.0
13	9782.5
14	9763.0
15	9416.5
16	9070.0
17	8599.5
18	8129.0
19	8404.0
20	8679.0
21	7947.0
22	7215.0
23	8198.5
24	9182.0
25	9954.0
26	12349.0
27	13972.0
28	17794.0
29	21616.0
30	26315.0
31	31014.0
32	36987.0
33	42960.0
34	49757.5
35	56555.0
36	63980.5
37	71406.0
38	78351.0
39	85296.0
40	108329.5
41	131363.0
42	146036.0
43	160709.0
44	161232.0
45	161755.0
46	157598.5
47	153442.0
48	142743.0
49	132044.0
50	125705.0
51	119366.0
52	110338.5
53	101311.0
54	93166.0
55	85021.0
56	77625.5
57	70230.0
58	63474.5
59	56719.0
60	49055.0
61	41391.0
62	36925.5
63	32460.0
64	26344.0
65	20228.0
66	19796.5
67	19365.0
68	14875.5
69	10386.0
70	9445.0
71	8504.0
72	7431.5
73	6359.0
74	5046.5
75	2991.5
76	2249.0
77	1528.0
78	807.0
79	599.0
80	391.0
81	262.5
82	134.0
83	85.5
84	37.0
85	24.5
86	12.0
87	8.5
88	5.0
89	3.0
90	1.0
91	1.0
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1751013.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.599138982791732
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.16764431486581	14.108815142203369
2	7.868264606320978	2.769493647200479
3	2.829688014206762	1.4940021791989424
4	1.488936106981137	1.0481597393303157
5	0.8627548309099068	0.7591871088629216
6	0.6122547641943668	0.6465094012759823
7	0.4397662963390791	0.541765571845332
8	0.34052766092652603	0.4794394905704727
9	0.27405011472066587	0.4340741451497116
>10	2.78126532660581	11.22358872968557
>50	0.878944206544403	11.151008881705875
>100	1.439633040382087	52.71434104235006
>500	0.014643645302258703	1.6587718128945594
>1k	9.762430201505801E-4	0.30003816902895314
>5k	6.508286801003867E-4	0.6708049386974857
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6384	0.3645889550791456	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5329	0.3043381174211728	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2475	0.14134675185164244	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03215281668382816	0.0
2	0.0	0.0	0.0	0.13917657949998086	0.0
3	0.0	0.0	0.0	0.1979996721897553	0.0
4	0.0	0.0	0.0	0.30148262748477594	0.0
5	0.0	0.0	0.0	0.5343763866973004	0.0
6	0.0	0.0	0.0	0.7611022876472077	0.0
7	0.0	0.0	0.0	0.92763446073787	0.0
8	0.0	0.0	0.0	1.3278599302232479	0.0
9	0.0	0.0	0.0	1.5151229602521512	0.0
10	0.0	0.0	0.0	1.9200885430319479	0.0
11	0.0	0.0	0.0	2.156066231375781	0.0
12	0.0	0.0	0.0	2.4308214730558824	0.0
13	5.710979872793634E-5	0.0	0.0	2.5221971510205807	0.0
14	5.710979872793634E-5	0.0	0.0	2.558690312407732	0.0
15	5.710979872793634E-5	0.0	0.0	2.6148292445572934	0.0
16	5.710979872793634E-5	0.0	0.0	2.729734159597901	0.0
17	5.710979872793634E-5	0.0	0.0	2.8582312067357583	0.0
18	1.1421959745587268E-4	0.0	0.0	3.0803883237874303	0.0
19	1.1421959745587268E-4	0.0	0.0	3.1790740559893043	0.0
20	1.1421959745587268E-4	0.0	0.0	3.2785593253733696	0.0
21	1.1421959745587268E-4	0.0	0.0	3.3763884105943247	0.0
22	1.1421959745587268E-4	0.0	0.0	3.476273448569485	0.0
23	1.1421959745587268E-4	0.0	0.0	3.5854673837372997	0.0
24	1.1421959745587268E-4	0.0	0.0	3.6692474584711823	0.0
25	1.1421959745587268E-4	0.0	0.0	3.7377221071459776	0.0
26	1.1421959745587268E-4	0.0	0.0	3.8098517829393614	0.0
27	1.1421959745587268E-4	0.0	0.0	3.8896341717622884	0.0
28	1.1421959745587268E-4	0.0	0.0	3.9718151721317887	0.0
29	1.1421959745587268E-4	0.0	0.0	4.053482184312738	0.0
30	1.1421959745587268E-4	0.0	0.0	4.165702938813133	0.0
31	1.1421959745587268E-4	0.0	0.0	4.257192836375287	0.0
32	1.7132939618380902E-4	0.0	0.0	4.343314412857015	0.0
33	1.7132939618380902E-4	0.0	0.0	4.429892867728566	0.0
34	1.7132939618380902E-4	0.0	0.0	4.520240569316162	0.0
35	1.7132939618380902E-4	0.0	0.0	4.630405371062351	0.0
36	1.7132939618380902E-4	0.0	0.0	4.722637696007968	0.0
37	1.7132939618380902E-4	0.0	0.0	4.815955107129416	0.0
38	1.7132939618380902E-4	0.0	0.0	4.913612862954187	0.0
39	1.7132939618380902E-4	0.0	0.0	5.01092795998659	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCCGC	30	2.1661563E-6	45.000004	26
TAAGTCG	30	2.1661563E-6	45.000004	15
CTAACCG	30	2.1661563E-6	45.000004	26
CTTATCG	30	2.1661563E-6	45.000004	21
CGTAGCA	30	2.1661563E-6	45.000004	40
ACGTACT	60	0.0	45.000004	19
TAGGCCG	30	2.1661563E-6	45.000004	5
TATCGAC	30	2.1661563E-6	45.000004	23
TGCACGA	30	2.1661563E-6	45.000004	2
AGTCGAT	30	2.1661563E-6	45.000004	26
CTAGACG	35	1.212411E-7	45.0	1
CTATGCG	35	1.212411E-7	45.0	1
TCGTTAC	20	7.0343E-4	45.0	39
ACCGGTA	50	2.1827873E-11	45.0	41
ACACGTC	20	7.0343E-4	45.0	29
GTCGATC	25	3.891646E-5	45.0	44
TAGCGAT	20	7.0343E-4	45.0	18
CCGTGTA	35	1.212411E-7	45.0	11
ATAGCCG	25	3.891646E-5	45.0	1
CGCGATA	20	7.0343E-4	45.0	11
>>END_MODULE