FastQCFastQC Report
Thu 26 May 2016
SRR1545177_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545177_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1942237
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120390.6198522631378148No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCGAT406.8193913E-945.034
ATGCGAT207.0344907E-445.034
TATACGA207.0344907E-445.018
TCGACTA207.0344907E-445.026
CGTTTTT84450.042.682061
GCGTAAG1450.041.8965531
CGCATCG700.041.78571321
CGGTCTA6050.041.6528931
CGACGGT6050.041.2809928
AGTACGG2350.040.2127652
ATTCGGC1750.039.85714317
ACGTTAG1300.039.807691
CTCACGA6450.039.76744524
TAGTACG850.039.7058831
TCACGAC6550.039.5038225
GTATCGC403.4598634E-739.37515
CACGACG6550.039.16030526
TAGGGAC15300.039.117655
TCGATAG1100.038.8636361
GGGCGAT32600.038.167187
Produced by FastQC (version 0.11.5)