##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545177_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1942237
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.004024740544022	31.0	31.0	33.0	28.0	34.0
2	31.28173183808155	31.0	31.0	34.0	28.0	34.0
3	31.390698457500296	31.0	31.0	34.0	28.0	34.0
4	35.18238042010321	37.0	35.0	37.0	32.0	37.0
5	35.02787970778026	37.0	35.0	37.0	32.0	37.0
6	35.070188138728696	37.0	35.0	37.0	32.0	37.0
7	35.715810686337456	37.0	35.0	37.0	35.0	37.0
8	35.71915064948305	37.0	35.0	37.0	35.0	37.0
9	37.36771568042417	39.0	37.0	39.0	35.0	39.0
10	36.524326845796885	39.0	35.0	39.0	32.0	39.0
11	36.261557163209226	38.0	35.0	39.0	31.0	39.0
12	35.75437240666304	37.0	35.0	39.0	30.0	39.0
13	35.54503853031324	37.0	35.0	39.0	30.0	39.0
14	36.44191208384971	38.0	35.0	40.0	30.0	41.0
15	36.659796924886095	38.0	35.0	40.0	31.0	41.0
16	36.73083511435525	38.0	35.0	40.0	31.0	41.0
17	36.655569840343894	38.0	35.0	40.0	31.0	41.0
18	36.57163518149433	38.0	35.0	40.0	31.0	41.0
19	36.507728974373364	38.0	35.0	40.0	30.0	41.0
20	36.39034577139659	38.0	35.0	40.0	30.0	41.0
21	36.268697383481005	38.0	34.0	40.0	30.0	41.0
22	36.20515879370026	38.0	34.0	40.0	30.0	41.0
23	36.12001521956383	38.0	34.0	40.0	30.0	41.0
24	36.05119354641066	38.0	34.0	40.0	30.0	41.0
25	35.935363192030636	38.0	34.0	40.0	30.0	41.0
26	35.787376102916376	38.0	34.0	40.0	29.0	41.0
27	35.67822207073596	38.0	34.0	40.0	29.0	41.0
28	35.65660524436513	38.0	34.0	40.0	29.0	41.0
29	35.61039564172653	37.0	34.0	40.0	29.0	41.0
30	35.51814994771493	37.0	34.0	40.0	29.0	41.0
31	35.37098768070014	37.0	34.0	40.0	27.0	41.0
32	35.22946272777215	37.0	34.0	40.0	27.0	41.0
33	35.00247189194727	37.0	33.0	40.0	26.0	41.0
34	34.79162069304621	37.0	33.0	40.0	25.0	41.0
35	34.64375974713693	37.0	33.0	40.0	24.0	41.0
36	34.515970502055104	37.0	33.0	40.0	24.0	41.0
37	34.47305555398234	37.0	33.0	40.0	24.0	41.0
38	34.39827580259258	37.0	33.0	40.0	24.0	41.0
39	34.32166156859333	37.0	33.0	40.0	23.0	41.0
40	34.197007883178	37.0	33.0	40.0	23.0	41.0
41	34.09578336732335	36.0	33.0	40.0	23.0	41.0
42	34.01258085393286	36.0	33.0	40.0	23.0	41.0
43	33.94637781074091	36.0	33.0	40.0	23.0	41.0
44	33.820600163625755	36.0	33.0	40.0	23.0	41.0
45	33.74868515016448	36.0	32.0	40.0	23.0	41.0
46	33.69427520946208	35.0	32.0	40.0	23.0	41.0
47	33.59012159690089	35.0	32.0	40.0	23.0	41.0
48	33.50350291957161	35.0	32.0	39.0	23.0	41.0
49	33.45027666551508	35.0	32.0	39.0	23.0	41.0
50	33.385182652786455	35.0	32.0	39.0	22.0	41.0
51	33.22838150030094	35.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	4.0
12	4.0
13	7.0
14	24.0
15	92.0
16	262.0
17	664.0
18	1425.0
19	2768.0
20	4670.0
21	7056.0
22	10649.0
23	15158.0
24	20654.0
25	28301.0
26	36250.0
27	42274.0
28	47341.0
29	53527.0
30	63273.0
31	75703.0
32	91415.0
33	114290.0
34	163534.0
35	204947.0
36	162284.0
37	196676.0
38	255448.0
39	343372.0
40	161.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.94058655045703	22.221747397459733	28.250929212037462	14.586736840045782
2	31.57802060201716	25.397260993380318	29.730048392652392	13.294670011950139
3	28.751022660983182	25.538386921884403	32.3748337612763	13.335756655856107
4	26.728457958529262	27.517084681220677	31.01408324524762	14.740374115002444
5	23.04023659316551	32.5094208379307	30.07624713152926	14.374095437374532
6	20.451983975179136	41.37728814763595	28.047967369584658	10.122760507600256
7	86.53949028877527	3.999872312184352	7.581515541100288	1.879121857940097
8	87.5516736628949	3.192710261415059	7.238508997614606	2.0171070780754357
9	82.30658771303399	5.5518456295498435	9.018981720562422	3.122584936853741
10	39.75328448587891	31.922829191288187	15.191812327743731	13.132073995089169
11	35.301047194549376	23.894921165645595	24.78523475765316	16.01879688215187
12	32.17542452337176	22.094368503946736	28.37717539105681	17.353031581624695
13	24.89248222539268	28.68470737608232	29.010929150253034	17.411881248271968
14	20.096466085240884	31.030404631360643	30.421725052091997	18.45140423130648
15	18.980845283042182	23.740820507486983	38.63704583941095	18.641288370059886
16	22.005141494060716	21.68113366185486	36.04524061687631	20.268484227208113
17	21.802025190540597	21.877144756278454	29.855316318245407	26.46551373493554
18	23.67280615084565	23.4687115938992	32.342860320341956	20.515621934913195
19	27.33796133015693	25.28409251806036	26.494964311770396	20.882981840012317
20	27.933666179771055	25.38299908816483	26.35234525961559	20.330989472448522
21	24.727620779544413	26.710231552586013	29.127701717143683	19.43444595072589
22	24.33848186395378	23.344164486620326	28.376917955944613	23.94043569348128
23	21.60101985494046	27.262275407172243	27.84382132561577	23.29288341227152
24	21.346879912183734	24.995559244314673	33.69794726390239	19.959613579599196
25	21.58711835888205	25.591984912242943	30.327709749119187	22.493186979755816
26	19.83975179136223	29.03466466759721	27.689875128524477	23.43570841251608
27	20.16103081137884	28.477626571834435	29.585524320667357	21.775818296119372
28	19.898549970987062	26.869738348100668	32.28277496515616	20.948936715756112
29	20.558253189492323	25.78485529829779	31.873041240590105	21.783850271619787
30	22.967485430459824	26.712342520505995	30.34907686343119	19.971095185602994
31	26.78972751523115	25.51506330071974	27.279060176487214	20.4161490075619
32	27.641837736589302	25.270139534979513	26.958656435852063	20.129366292579125
33	26.817684968415286	25.425733316788836	26.754047008681226	21.002534706114652
34	21.966577714254235	26.371704380052485	28.867640766806524	22.79407713888676
35	22.01276157338162	26.11807930752014	29.281493453167663	22.587665665930572
36	26.670792493398075	26.585632958284698	26.11416629381481	20.629408254502412
37	22.097921108494997	30.144107027103285	26.812381805104113	20.945590059297604
38	22.434697722265614	29.698898744077063	25.864351260942925	22.0020522727144
39	21.963334031840603	28.827223454192254	25.74567367422204	23.463768839745097
40	23.8381824669183	26.082141365858032	26.9015573279677	23.17811883925597
41	21.13907828962171	25.16083258634245	27.67489240499486	26.025196719040984
42	22.27091750388856	26.434518547427526	26.34899860315708	24.94556534552683
43	22.561922154711294	26.38586331122309	26.85182086429205	24.200393669773565
44	22.407872983575125	27.262944738463947	28.36090549196622	21.968276785994707
45	20.781964301987863	30.030063272401875	26.076941176591735	23.11103124901853
46	22.492208726329483	28.435870596636764	27.62690650008212	21.44501417695163
47	21.326491051298067	27.99292774259784	27.649663763999964	23.03091744210413
48	22.079746189574188	27.200748415358166	28.534159322471975	22.18534607259567
49	23.17106511718189	25.615617455542246	29.028074328725072	22.185243098550796
50	21.444499306727245	28.325688368618245	27.764067927858445	22.465744396796065
51	20.95423987906728	28.558718632175168	27.484390421972186	23.00265106678536
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	975.0
1	1707.0
2	2439.0
3	11340.0
4	20241.0
5	14502.0
6	8763.0
7	8895.0
8	9027.0
9	9728.0
10	10429.0
11	10313.5
12	10198.0
13	10255.5
14	10313.0
15	9632.0
16	8951.0
17	8710.5
18	8470.0
19	8325.5
20	8181.0
21	8282.0
22	8383.0
23	9029.0
24	9675.0
25	11149.0
26	14441.0
27	16259.0
28	19461.0
29	22663.0
30	26738.5
31	30814.0
32	35829.0
33	40844.0
34	46098.5
35	51353.0
36	56727.5
37	62102.0
38	71485.5
39	80869.0
40	93532.0
41	106195.0
42	120711.0
43	135227.0
44	135279.5
45	135332.0
46	142595.5
47	149859.0
48	149517.0
49	149175.0
50	143403.5
51	137632.0
52	129718.5
53	121805.0
54	115195.5
55	108586.0
56	105336.0
57	102086.0
58	96092.5
59	90099.0
60	80481.5
61	70864.0
62	64994.5
63	59125.0
64	53102.0
65	47079.0
66	39399.5
67	31720.0
68	26361.5
69	21003.0
70	18514.5
71	16026.0
72	13829.5
73	11633.0
74	9394.0
75	5641.5
76	4128.0
77	3064.5
78	2001.0
79	1485.5
80	970.0
81	780.0
82	590.0
83	429.5
84	269.0
85	171.5
86	74.0
87	50.0
88	26.0
89	15.5
90	5.0
91	2.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1942237.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.11962496213312
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.4866656215774	16.364893174939287
2	7.0480065827221505	2.977025115154746
3	2.839120596929386	1.7988348668824843
4	1.7070969497829915	1.442129894136727
5	1.1332114767198718	1.1966500695554372
6	0.8281029723006199	1.0493534523010082
7	0.6590957194466907	0.974389808620299
8	0.5424219107131861	0.9164597860404919
9	0.43008233590278633	0.8174859873394508
>10	5.160423336283263	25.14405104589736
>50	1.3225686148208424	19.802141678158673
>100	0.838560151331527	26.12254252749362
>500	0.0031772899527014414	0.4135103942078708
>1k	0.0012220345971928619	0.3591052069035273
>5k	0.0	0.0
>10k+	2.444069194385724E-4	0.6214269923690621
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12039	0.6198522631378148	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.031613031777275376	0.0
2	0.0	0.0	0.0	0.11059412419802526	0.0
3	0.0	0.0	0.0	0.16264750388340868	0.0
4	0.0	0.0	0.0	0.2299410422106056	0.0
5	0.0	0.0	0.0	0.39608966361983633	0.0
6	0.0	0.0	0.0	0.609194449493033	0.0
7	0.0	0.0	0.0	0.7667447381550243	0.0
8	0.0	0.0	0.0	1.1659751101436127	0.0
9	0.0	0.0	0.0	1.3773293372538984	0.0
10	0.0	0.0	0.0	1.7093691449601671	0.0
11	0.0	0.0	0.0	1.9320505170069358	0.0
12	0.0	0.0	0.0	2.1475237059123065	0.0
13	0.0	0.0	0.0	2.2347427219232254	0.0
14	0.0	0.0	0.0	2.2713499948770415	0.0
15	0.0	0.0	0.0	2.3181516982736916	0.0
16	0.0	0.0	0.0	2.4095411631021344	0.0
17	0.0	0.0	0.0	2.5126696690465686	0.0
18	0.0	0.0	0.0	2.679281673657746	0.0
19	5.148702243855925E-5	0.0	0.0	2.7460603417605576	0.0
20	5.148702243855925E-5	0.0	0.0	2.8331763837265997	0.0
21	5.148702243855925E-5	0.0	0.0	2.9285303492828114	0.0
22	5.148702243855925E-5	0.0	0.0	3.0278488155667924	0.0
23	5.148702243855925E-5	0.0	0.0	3.135096283306311	0.0
24	5.148702243855925E-5	0.0	0.0	3.2155190123553408	0.0
25	5.148702243855925E-5	0.0	0.0	3.2880642269712705	0.0
26	5.148702243855925E-5	0.0	0.0	3.3599401102954993	0.0
27	5.148702243855925E-5	0.0	0.0	3.44247380726451	0.0
28	5.148702243855925E-5	0.0	0.0	3.5192409577204016	0.0
29	5.148702243855925E-5	0.0	0.0	3.614543436254175	0.0
30	5.148702243855925E-5	0.0	0.0	3.7463502136968865	0.0
31	5.148702243855925E-5	0.0	0.0	3.849066823461812	0.0
32	5.148702243855925E-5	0.0	0.0	3.945810938623865	0.0
33	5.148702243855925E-5	0.0	0.0	4.049145392658054	0.0
34	5.148702243855925E-5	0.0	0.0	4.156907730621958	0.0
35	1.029740448771185E-4	0.0	0.0	4.276821005881363	0.0
36	1.029740448771185E-4	0.0	0.0	4.3815456095213925	0.0
37	1.029740448771185E-4	0.0	0.0	4.491933785629663	0.0
38	1.029740448771185E-4	0.0	0.0	4.626520862284057	0.0
39	1.029740448771185E-4	0.0	0.0	4.833910588666574	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTCGAT	40	6.8193913E-9	45.0	34
ATGCGAT	20	7.0344907E-4	45.0	34
TATACGA	20	7.0344907E-4	45.0	18
TCGACTA	20	7.0344907E-4	45.0	26
CGTTTTT	8445	0.0	42.68206	1
GCGTAAG	145	0.0	41.896553	1
CGCATCG	70	0.0	41.785713	21
CGGTCTA	605	0.0	41.65289	31
CGACGGT	605	0.0	41.28099	28
AGTACGG	235	0.0	40.212765	2
ATTCGGC	175	0.0	39.857143	17
ACGTTAG	130	0.0	39.80769	1
CTCACGA	645	0.0	39.767445	24
TAGTACG	85	0.0	39.705883	1
TCACGAC	655	0.0	39.50382	25
GTATCGC	40	3.4598634E-7	39.375	15
CACGACG	655	0.0	39.160305	26
TAGGGAC	1530	0.0	39.11765	5
TCGATAG	110	0.0	38.863636	1
GGGCGAT	3260	0.0	38.16718	7
>>END_MODULE