FastQCFastQC Report
Thu 26 May 2016
SRR1545176_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545176_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences954616
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28560.29917788932932193No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC27420.28723591475525234No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCT207.032684E-445.00000431
GGTACGT207.032684E-445.0000048
CTCACAT207.032684E-445.00000423
ACAGTCG406.8121153E-945.00000412
TACTTCG207.032684E-445.0000041
TCCGATA207.032684E-445.00000415
ACGCACT207.032684E-445.00000428
TCACACG406.8121153E-945.0000041
GACAATC207.032684E-445.00000431
CCGGAAC207.032684E-445.00000419
CGTCTTA207.032684E-445.00000422
TGTATCG302.165114E-645.00000414
GATCACG302.165114E-645.0000049
TATGCGT302.165114E-645.00000420
ATCGTTA207.032684E-445.00000429
CCCACCG207.032684E-445.00000429
CGTTACC207.032684E-445.00000435
GTGTCGT207.032684E-445.00000420
TCGCTGA207.032684E-445.00000443
TCTCGAG207.032684E-445.0000041