##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545176_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	954616
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.09161065810755	31.0	31.0	33.0	30.0	34.0
2	31.363771401275486	31.0	31.0	34.0	30.0	34.0
3	31.489750852698887	31.0	31.0	34.0	30.0	34.0
4	35.247491137797816	37.0	35.0	37.0	33.0	37.0
5	35.10152040192077	37.0	35.0	37.0	32.0	37.0
6	35.15216799215601	37.0	35.0	37.0	32.0	37.0
7	35.747629413292884	37.0	35.0	37.0	35.0	37.0
8	35.75853327411232	37.0	35.0	37.0	35.0	37.0
9	37.49309565312125	39.0	37.0	39.0	35.0	39.0
10	36.694745321679086	39.0	37.0	39.0	32.0	39.0
11	36.3519970333621	38.0	35.0	39.0	32.0	39.0
12	35.28790319877312	37.0	35.0	39.0	30.0	39.0
13	34.77855598481484	37.0	33.0	39.0	27.0	39.0
14	35.665448724932325	37.0	34.0	40.0	27.0	41.0
15	36.102705171503516	37.0	34.0	40.0	30.0	41.0
16	36.338388420055814	37.0	35.0	40.0	31.0	41.0
17	36.272733748439165	37.0	35.0	40.0	31.0	41.0
18	36.2082188021152	37.0	35.0	40.0	31.0	41.0
19	36.14259346166417	37.0	35.0	40.0	31.0	41.0
20	35.97786335028954	36.0	34.0	40.0	30.0	41.0
21	35.82241760037544	36.0	34.0	40.0	30.0	41.0
22	35.72135706922993	36.0	34.0	40.0	30.0	41.0
23	35.70400139951562	36.0	34.0	40.0	30.0	41.0
24	35.673228816613175	36.0	34.0	40.0	30.0	41.0
25	35.58408302395937	36.0	34.0	40.0	30.0	41.0
26	35.3837364971884	35.0	34.0	40.0	29.0	41.0
27	35.31304943558457	35.0	34.0	40.0	29.0	41.0
28	35.33061985133289	36.0	34.0	40.0	29.0	41.0
29	35.400516019006595	36.0	34.0	40.0	29.0	41.0
30	35.3623383643266	36.0	34.0	40.0	29.0	41.0
31	35.17092946273685	36.0	34.0	40.0	29.0	41.0
32	34.96739317170464	35.0	34.0	40.0	28.0	41.0
33	34.75772352443286	35.0	33.0	40.0	27.0	41.0
34	34.61898606350616	35.0	34.0	40.0	26.0	41.0
35	34.45573508091211	35.0	33.0	40.0	25.0	41.0
36	34.241903550747104	35.0	33.0	40.0	24.0	41.0
37	34.183691662406666	35.0	33.0	40.0	24.0	41.0
38	34.211084876012976	35.0	33.0	40.0	24.0	41.0
39	34.20635417801503	35.0	33.0	40.0	24.0	41.0
40	34.001327235244325	35.0	33.0	40.0	23.0	41.0
41	34.00888629564139	35.0	33.0	40.0	23.0	41.0
42	33.94146232621284	35.0	33.0	40.0	23.0	41.0
43	33.89801867976233	35.0	33.0	40.0	23.0	41.0
44	33.78940851609443	35.0	33.0	39.0	23.0	41.0
45	33.73666479505896	35.0	33.0	39.0	23.0	41.0
46	33.68701341691319	35.0	33.0	39.0	23.0	41.0
47	33.600569234121366	35.0	33.0	39.0	23.0	41.0
48	33.59970396473556	35.0	33.0	39.0	23.0	41.0
49	33.62238219346837	35.0	33.0	39.0	24.0	41.0
50	33.52937725745221	35.0	33.0	39.0	24.0	41.0
51	33.346938454834195	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	4.0
14	6.0
15	30.0
16	106.0
17	269.0
18	620.0
19	1148.0
20	2048.0
21	3336.0
22	5136.0
23	7188.0
24	10202.0
25	13482.0
26	17081.0
27	19976.0
28	22229.0
29	25774.0
30	31012.0
31	37914.0
32	47182.0
33	60699.0
34	100606.0
35	145317.0
36	65628.0
37	77553.0
38	103303.0
39	156675.0
40	90.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.310147745271394	24.394625692425016	29.65527500062852	12.639951561675062
2	29.44712847888575	26.077815582391246	31.950962481249007	12.524093457474
3	27.51022400630201	26.62431805039932	32.391977507186134	13.473480436112531
4	24.7036504730698	28.31557401091119	32.31257385168487	14.668201664334141
5	23.10426391344792	32.66863325148541	30.72816713736204	13.498935697704626
6	19.59384715948612	42.115782681203754	27.271803531472337	11.01856662783779
7	87.748476874471	4.343526611747551	6.304210279316499	1.6037862344649576
8	89.68171495135216	3.203487056575628	5.411809565312126	1.7029884267600794
9	85.87693899955585	5.0340660537849775	6.670116570432509	2.4188783762266715
10	50.92539827532746	26.614680667409722	12.4229009360832	10.037020121179616
11	47.381774451716716	19.987722812104554	20.049003997418858	12.581498738759878
12	44.75988250773086	21.414474511217076	21.15122729977289	12.674415681279175
13	21.900848089703086	43.793839617186386	20.376989281554046	13.92832301155648
14	14.048999807252342	45.35446713652401	24.76105575435568	15.835477301867975
15	12.04976660772499	24.10361862780427	48.40281327779966	15.443801486671079
16	14.238604842156427	17.458328793986272	47.20295909559446	21.100107268262843
17	14.694390205066748	18.82358979945863	28.70620228447879	37.77581771099584
18	19.73673183772323	23.14438475785028	35.09976786477495	22.019115539651544
19	27.6165494816764	24.957365055687315	24.88948435810839	22.536601104527897
20	32.38275914084826	21.672379260351807	25.152626815389645	20.792234783410294
21	20.934700444995684	30.391592011866553	26.747194683516724	21.92651285962104
22	22.31389375413779	24.53300594165612	23.79595565127758	29.357144652928508
23	17.639867758344714	32.46394361711935	23.673498034811903	26.222690589724035
24	18.270278310860075	23.43968674315117	40.97270525530685	17.317329690681905
25	15.792842357555289	26.164237766808853	36.28893712236124	21.753982753274613
26	16.190070143387498	35.232386635044875	26.42821825739355	22.149324964174077
27	15.7068391897894	37.402159611822974	26.686856285668792	20.20414491271883
28	13.205624041499409	29.861640701601484	38.203843220729595	18.728892036169515
29	14.283125361401863	23.121967366983164	37.891571061033964	24.70333621058101
30	16.801415438249517	32.500398065819134	31.838770772750507	18.859415723180838
31	28.25911151709169	28.375702900433264	24.478848039421088	18.886337543053962
32	29.965347322902613	26.218081406555093	25.080241688804712	18.73632958173758
33	28.888055511326023	27.990836105826844	23.107511292498764	20.01359709034837
34	19.0065953220981	27.699724287043164	26.84629212164891	26.44738826920982
35	19.41827918241471	26.47357680994243	30.892840681488682	23.21530332615418
36	30.168570398987654	26.051627041658637	24.297413829225572	19.482388730128136
37	19.284612870515474	35.0338774962917	28.212286406261782	17.46922322693104
38	18.791325517276057	32.90024470572461	25.912723021612877	22.39570675538646
39	19.438287227534424	33.893314170305125	26.632279366782036	20.036119235378415
40	24.13274028509893	27.748958743620474	24.965221617907098	23.153079353373503
41	16.78580706797288	25.26722786963554	28.062906969922985	29.884058092468596
42	20.881904346878745	25.300749201773275	24.353771568882145	29.46357488246583
43	21.703281738416287	26.554970794539372	25.15168402792327	26.59006343912107
44	18.993396297568864	29.828224123626672	28.19908738173255	22.979292197071914
45	16.09652467589062	39.9719887368324	21.676359918543163	22.255126668733816
46	20.982468343291963	34.422636955592615	24.990991141987983	19.60390355912744
47	20.395635522555665	29.57901397001517	25.693158296110685	24.33219221131848
48	21.434063539685067	24.743352300820433	30.862042957587132	22.960541201907365
49	20.617085822990607	24.36372321436054	31.808496819663613	23.21069414298524
50	19.13135753014825	33.72623128043109	26.290990303954683	20.851420885465988
51	16.770722468510897	33.822290847838296	24.186374416519314	25.220612267131497
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	782.0
1	921.5
2	1061.0
3	3039.5
4	5018.0
5	3946.0
6	2874.0
7	3240.5
8	3607.0
9	3938.0
10	4269.0
11	4580.5
12	4892.0
13	4752.5
14	4613.0
15	4319.5
16	4026.0
17	3848.0
18	3670.0
19	3412.5
20	3155.0
21	3510.5
22	3866.0
23	3564.5
24	3263.0
25	3858.5
26	5484.0
27	6514.0
28	8709.5
29	10905.0
30	12385.5
31	13866.0
32	16899.0
33	19932.0
34	26340.5
35	32749.0
36	33171.0
37	33593.0
38	37933.0
39	42273.0
40	57904.5
41	73536.0
42	86117.5
43	98699.0
44	98894.5
45	99090.0
46	92187.0
47	85284.0
48	81559.5
49	77835.0
50	72696.0
51	67557.0
52	61309.0
53	55061.0
54	48106.0
55	41151.0
56	38474.5
57	35798.0
58	33294.0
59	30790.0
60	27651.5
61	24513.0
62	21450.5
63	18388.0
64	15087.5
65	11787.0
66	10622.5
67	9458.0
68	7796.5
69	6135.0
70	4857.5
71	3580.0
72	3319.5
73	3059.0
74	2440.0
75	1229.5
76	638.0
77	650.5
78	663.0
79	444.5
80	226.0
81	140.0
82	54.0
83	43.5
84	33.0
85	35.5
86	38.0
87	31.5
88	25.0
89	16.0
90	7.0
91	4.5
92	2.0
93	3.5
94	5.0
95	2.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	954616.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.474351632938355
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.68621267370617	14.099392521903889
2	7.254771232314669	2.535448470599841
3	2.426726279259854	1.2721640496203652
4	1.1509537695733385	0.804486835311217
5	0.7137200337676839	0.6235897418764572
6	0.46974642017651197	0.49251084746870266
7	0.40601583063418784	0.496640437512894
8	0.32477812645213316	0.45402297474491937
9	0.2677972708440572	0.42116253093632566
>10	3.4253534985435588	14.456095916242695
>50	1.272557820252845	16.05495058142099
>100	1.5959570206760996	47.2446949377384
>500	0.004207796288034915	0.4568221405907939
>1k	0.0012022275108671185	0.588018014032482
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2856	0.29917788932932193	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2742	0.28723591475525234	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02482673661451306	0.0
2	0.0	0.0	0.0	0.10454465460457399	0.0
3	0.0	0.0	0.0	0.1526268153896436	0.0
4	0.0	0.0	0.0	0.2262689919297393	0.0
5	0.0	0.0	0.0	0.3801528572745481	0.0
6	0.0	0.0	0.0	0.5413695140244873	0.0
7	0.0	0.0	0.0	0.6503138434721396	0.0
8	0.0	0.0	0.0	0.9792419150737051	0.0
9	0.0	0.0	0.0	1.13700168444694	0.0
10	0.0	0.0	0.0	1.4048580790600618	0.0
11	0.0	0.0	0.0	1.5858732726038534	0.0
12	0.0	0.0	0.0	1.769612074383836	0.0
13	0.0	0.0	0.0	1.837283263636897	0.0
14	0.0	0.0	0.0	1.8634718043695055	0.0
15	0.0	0.0	0.0	1.9020213363279057	0.0
16	0.0	0.0	0.0	1.9887577832343057	0.0
17	0.0	0.0	0.0	2.0867029255742624	0.0
18	0.0	0.0	0.0	2.261013852690506	0.0
19	1.0475416293043486E-4	0.0	0.0	2.327427991988402	0.0
20	1.0475416293043486E-4	0.0	0.0	2.401174922691428	0.0
21	1.0475416293043486E-4	0.0	0.0	2.4855020238504277	0.0
22	1.0475416293043486E-4	0.0	0.0	2.567524533424958	0.0
23	1.0475416293043486E-4	0.0	0.0	2.6598129509666713	0.0
24	1.0475416293043486E-4	0.0	0.0	2.727169877730941	0.0
25	2.0950832586086971E-4	0.0	0.0	2.783108600735793	0.0
26	2.0950832586086971E-4	0.0	0.0	2.8395710945552977	0.0
27	2.0950832586086971E-4	0.0	0.0	2.903052117291141	0.0
28	2.0950832586086971E-4	0.0	0.0	2.9686282232855934	0.0
29	2.0950832586086971E-4	0.0	0.0	3.036718429190376	0.0
30	2.0950832586086971E-4	0.0	0.0	3.1336160299010283	0.0
31	2.0950832586086971E-4	0.0	0.0	3.2090390272109413	0.0
32	2.0950832586086971E-4	0.0	0.0	3.2836239912174108	0.0
33	2.0950832586086971E-4	0.0	0.0	3.3516094429592633	0.0
34	2.0950832586086971E-4	0.0	0.0	3.427241948595037	0.0
35	2.0950832586086971E-4	0.0	0.0	3.5400621820711153	0.0
36	2.0950832586086971E-4	0.0	0.0	3.6203038708758286	0.0
37	2.0950832586086971E-4	0.0	0.0	3.699183755562446	0.0
38	2.0950832586086971E-4	0.0	0.0	3.784977415002472	0.0
39	3.1426248879130457E-4	0.0	0.0	3.867104678739933	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGCT	20	7.032684E-4	45.000004	31
GGTACGT	20	7.032684E-4	45.000004	8
CTCACAT	20	7.032684E-4	45.000004	23
ACAGTCG	40	6.8121153E-9	45.000004	12
TACTTCG	20	7.032684E-4	45.000004	1
TCCGATA	20	7.032684E-4	45.000004	15
ACGCACT	20	7.032684E-4	45.000004	28
TCACACG	40	6.8121153E-9	45.000004	1
GACAATC	20	7.032684E-4	45.000004	31
CCGGAAC	20	7.032684E-4	45.000004	19
CGTCTTA	20	7.032684E-4	45.000004	22
TGTATCG	30	2.165114E-6	45.000004	14
GATCACG	30	2.165114E-6	45.000004	9
TATGCGT	30	2.165114E-6	45.000004	20
ATCGTTA	20	7.032684E-4	45.000004	29
CCCACCG	20	7.032684E-4	45.000004	29
CGTTACC	20	7.032684E-4	45.000004	35
GTGTCGT	20	7.032684E-4	45.000004	20
TCGCTGA	20	7.032684E-4	45.000004	43
TCTCGAG	20	7.032684E-4	45.000004	1
>>END_MODULE