FastQCFastQC Report
Thu 26 May 2016
SRR1545175_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545175_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1652474
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67220.4067840099148307No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC23720.14354234922909528No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGAA302.1660817E-645.0000048
TATAGCG1400.045.0000041
TACGTTG302.1660817E-645.0000041
GTCGACT253.8915496E-545.034
CTATACG406.8175723E-945.01
CGGTCGA253.8915496E-545.031
TTCGATC253.8915496E-545.025
ATCGCAC453.8562575E-1045.011
ATCGACG207.034185E-445.01
TACGTAC207.034185E-445.028
CCCGATA207.034185E-445.016
CGTTTTT51950.043.310881
ACTACGG1100.042.9545442
TACGGGA8000.042.18754
CGTATGG1900.041.447372
CGACGGT5150.041.0679628
ACGTAGG2250.041.02
CTATGCG1100.040.9090921
AGGTACG501.0822987E-940.51
TCGATAG1000.040.51
Produced by FastQC (version 0.11.5)