##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545175_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1652474
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.06026115993353	31.0	31.0	33.0	30.0	34.0
2	31.343641110238345	31.0	31.0	34.0	30.0	34.0
3	31.461547352636106	31.0	31.0	34.0	30.0	34.0
4	35.23696530172336	37.0	35.0	37.0	33.0	37.0
5	35.091487067270045	37.0	35.0	37.0	32.0	37.0
6	35.14544555617819	37.0	35.0	37.0	32.0	37.0
7	35.751432095149454	37.0	35.0	37.0	35.0	37.0
8	35.76553398117005	37.0	35.0	37.0	35.0	37.0
9	37.46651687106726	39.0	37.0	39.0	35.0	39.0
10	36.59977343062584	39.0	35.0	39.0	32.0	39.0
11	36.331466637296565	38.0	35.0	39.0	32.0	39.0
12	35.74192997892857	37.0	35.0	39.0	31.0	39.0
13	35.48360639864833	37.0	35.0	39.0	30.0	39.0
14	36.40555554883163	38.0	35.0	40.0	30.0	41.0
15	36.64597869618523	38.0	35.0	40.0	31.0	41.0
16	36.75481490177758	38.0	35.0	40.0	31.0	41.0
17	36.71266900417193	38.0	35.0	40.0	31.0	41.0
18	36.62625130561812	38.0	35.0	40.0	31.0	41.0
19	36.58450359884634	38.0	35.0	40.0	31.0	41.0
20	36.468648825942196	38.0	35.0	40.0	31.0	41.0
21	36.34760546913295	38.0	35.0	40.0	30.0	41.0
22	36.27784279813177	38.0	35.0	40.0	30.0	41.0
23	36.24104766550033	38.0	34.0	40.0	30.0	41.0
24	36.160345034173005	38.0	34.0	40.0	30.0	41.0
25	36.05659816735392	38.0	34.0	40.0	30.0	41.0
26	35.922003613975164	38.0	34.0	40.0	30.0	41.0
27	35.83761317878526	38.0	34.0	40.0	30.0	41.0
28	35.818935123941436	38.0	34.0	40.0	29.0	41.0
29	35.79255830954072	38.0	34.0	40.0	29.0	41.0
30	35.71749812705071	38.0	34.0	40.0	29.0	41.0
31	35.576291669339426	38.0	34.0	40.0	29.0	41.0
32	35.454317586842514	38.0	34.0	40.0	28.0	41.0
33	35.28125586242204	37.0	34.0	40.0	27.0	41.0
34	35.12615690171222	37.0	34.0	40.0	27.0	41.0
35	34.98895534816282	37.0	34.0	40.0	26.0	41.0
36	34.838557217844276	37.0	33.0	40.0	25.0	41.0
37	34.78939577869304	37.0	33.0	40.0	25.0	41.0
38	34.73902161244292	37.0	33.0	40.0	25.0	41.0
39	34.68641866679899	37.0	33.0	40.0	25.0	41.0
40	34.536661393764746	37.0	33.0	40.0	24.0	41.0
41	34.48448568631034	37.0	33.0	40.0	24.0	41.0
42	34.41189029297889	36.0	33.0	40.0	24.0	41.0
43	34.367251769165506	36.0	33.0	40.0	24.0	41.0
44	34.23965278727532	36.0	33.0	40.0	24.0	41.0
45	34.156545882113726	36.0	33.0	40.0	24.0	41.0
46	34.09482932863089	36.0	33.0	40.0	23.0	41.0
47	34.000628754219434	35.0	33.0	40.0	23.0	41.0
48	33.93371514468609	35.0	33.0	40.0	23.0	41.0
49	33.91795695423952	36.0	33.0	39.0	24.0	41.0
50	33.87560833029748	35.0	33.0	39.0	24.0	41.0
51	33.696072676483865	35.0	32.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	1.0
14	14.0
15	53.0
16	157.0
17	417.0
18	999.0
19	1863.0
20	3299.0
21	5100.0
22	7609.0
23	11154.0
24	15439.0
25	21119.0
26	27381.0
27	32348.0
28	36872.0
29	42826.0
30	50777.0
31	62170.0
32	76452.0
33	95833.0
34	144844.0
35	188052.0
36	134470.0
37	164649.0
38	219003.0
39	309381.0
40	190.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.66213326200594	24.14730882301325	28.79724582656066	13.393312088420151
2	30.998914355082135	25.860316107847993	30.341415356610757	12.799354180459119
3	28.9073837167786	25.833628849833644	31.453142379244696	13.80584505414306
4	25.634593948225508	28.491340862246545	31.37913213763121	14.494933051896734
5	23.21119727148506	32.55433973545121	30.123439158498105	14.111023834565628
6	19.982039051749073	42.17415826209671	27.36890262721229	10.474900058941925
7	88.2635369754683	4.092590866785196	5.976432912106333	1.6674392456401737
8	90.14229573354861	2.660193140709022	5.623810117436038	1.5737010083063334
9	85.40079904434201	4.898352409780729	7.156179159248496	2.544669386628776
10	42.08889217016425	30.697548040090194	14.457958188752137	12.755601600993419
11	36.88251675971907	23.811932895767196	23.205569346325568	16.099980998188173
12	35.68031932726324	21.777165631652903	26.642718735665433	15.89979630541842
13	24.25526816155655	31.92274129577833	25.94606632237482	17.875924220290305
14	19.208532176603082	33.88192491984745	27.576167612924625	19.333375290624847
15	17.15421846274132	24.385860231386395	39.7406555261989	18.719265779673385
16	19.836499696818226	21.109742119997048	37.65094034762423	21.4028178355605
17	19.939678324742175	21.12299497601778	28.865083505095996	30.072243194144054
18	22.405435728489525	23.848060544371652	32.230764296442786	21.515739430696033
19	27.47208125513624	26.02951695457841	25.940075305269556	20.5583264850158
20	29.14006513869507	24.490672773066326	25.327660223398375	21.041601864840235
21	23.478493458898598	26.750617558884436	28.12479954298827	21.646089439228696
22	22.658813391315082	24.688557883512843	26.29850757107222	26.35412115409985
23	20.941993641049724	28.898730025404333	25.743642562606126	24.415633770939817
24	19.980102561371616	25.332985571936383	34.972713640275124	19.714198226416876
25	20.72407795826137	25.916050721524215	31.163152945220318	22.196718374994102
26	19.35733935904589	30.443867800643158	26.752614564586192	23.446178275724762
27	19.515102809484446	29.97844444148592	27.966733515928237	22.539719233101398
28	18.311695070542715	27.5450022209124	33.31005510525431	20.83324760329058
29	19.260212263551498	25.174072330336212	32.98805306467757	22.57766234143472
30	21.099636060839686	28.041288395460384	30.446167382966387	20.412908160733544
31	26.449856397135445	26.2674026943843	26.44477310989462	20.837967798585634
32	27.60491239196502	25.6169234735312	26.384318300923344	20.393845833580436
33	26.425347690795743	26.05335999235086	25.854506636715612	21.66678568013778
34	20.824593911916313	26.394000752810637	28.23330351945023	24.54810181582282
35	21.063447896910933	25.765670140649714	29.72070967531108	23.45017228712827
36	26.860755449102374	26.5557582146527	26.00422154902286	20.57926478722207
37	21.18514421406933	31.53042044837014	26.744021388536222	20.540413949024313
38	21.34980641147758	30.68284281628637	25.99998547632217	21.967365295913886
39	21.202270050844977	29.874297568373237	25.504849092935807	23.41858328784598
40	23.929272109576306	26.383229025086024	26.824809346470808	22.862689518866862
41	20.366674452971726	24.2382028401052	28.087280041925016	27.307842664998056
42	21.45377173861737	26.192363692257793	25.46636134668382	26.887503222441016
43	20.956577834204957	26.920847166127874	26.464259044317796	25.658315955349376
44	21.320577509842817	27.82609590226533	28.56644037969735	22.286886208194503
45	19.734289314083007	31.83886705630467	25.0853568649189	23.34148676469342
46	21.452077309537092	30.471220727224757	26.413123595288035	21.663578367950116
47	21.20947137443615	27.838743605043103	27.40406203062802	23.54772298989273
48	20.97751613641122	26.62353537786374	29.417769961887448	22.981178523837592
49	21.489596810600347	26.12470755969534	29.791210028115422	22.59448560158889
50	21.009891834909354	29.315438548503636	27.815868812459378	21.85880080412763
51	19.697132904965525	30.866083218253358	26.38710200584094	23.049681870940177
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	642.0
1	1188.5
2	1735.0
3	6723.0
4	11711.0
5	8701.0
6	5691.0
7	5831.5
8	5972.0
9	6307.0
10	6642.0
11	6685.5
12	6729.0
13	6546.0
14	6363.0
15	6191.0
16	6019.0
17	5857.5
18	5696.0
19	5271.5
20	4847.0
21	5427.0
22	6007.0
23	6014.5
24	6022.0
25	7647.0
26	10925.0
27	12578.0
28	17627.5
29	22677.0
30	25981.5
31	29286.0
32	33715.5
33	38145.0
34	45799.0
35	53453.0
36	56091.5
37	58730.0
38	64676.0
39	70622.0
40	87024.0
41	103426.0
42	116030.0
43	128634.0
44	130757.0
45	132880.0
46	129355.5
47	125831.0
48	124327.0
49	122823.0
50	118189.5
51	113556.0
52	107081.0
53	100606.0
54	93608.0
55	86610.0
56	84462.0
57	82314.0
58	76980.5
59	71647.0
60	64957.5
61	58268.0
62	53059.5
63	47851.0
64	42467.0
65	37083.0
66	30613.0
67	24143.0
68	20272.5
69	16402.0
70	14636.5
71	12871.0
72	10588.0
73	8305.0
74	6860.5
75	4065.0
76	2714.0
77	1994.5
78	1275.0
79	879.5
80	484.0
81	394.0
82	304.0
83	225.0
84	146.0
85	87.5
86	29.0
87	18.5
88	8.0
89	8.0
90	8.0
91	4.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1652474.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.857846889084907
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.78962782004629	14.669448909893326
2	7.015399515787105	2.6459065986894728
3	2.707745715472497	1.5318676235086803
4	1.5537686405601125	1.1720292449897682
5	1.0530438268199802	0.9929069626833615
6	0.759594141148412	0.8594586006973621
7	0.5777284231614813	0.7626299903216168
8	0.4650349118117924	0.7015645732020707
9	0.39233877538134965	0.6658798099313311
>10	5.117590889458768	22.644122522676966
>50	1.4407197858346348	19.317865347008624
>100	1.1219502856033559	32.68308306080401
>500	0.003531174003325311	0.5130482912970754
>1k	0.001605079092420596	0.43326095820804356
>5k	3.210158184841192E-4	0.4069275060883706
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6722	0.4067840099148307	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2372	0.14354234922909528	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.019364903774582838	0.0
2	0.0	0.0	0.0	0.0657196421849905	0.0
3	0.0	0.0	0.0	0.09815585600741676	0.0
4	0.0	0.0	0.0	0.14378441052627758	0.0
5	0.0	0.0	0.0	0.24974674336782304	0.0
6	0.0	0.0	0.0	0.39316806194832715	0.0
7	0.0	0.0	0.0	0.4958625672779118	0.0
8	0.0	0.0	0.0	0.7874253997339746	0.0
9	0.0	0.0	0.0	0.9353248523123511	0.0
10	0.0	0.0	0.0	1.1631650482851772	0.0
11	0.0	0.0	0.0	1.3225624124797122	0.0
12	0.0	0.0	0.0	1.4720352634897735	0.0
13	6.0515324295571366E-5	0.0	0.0	1.533518832974074	0.0
14	6.0515324295571366E-5	0.0	0.0	1.559903514366943	0.0
15	6.0515324295571366E-5	0.0	0.0	1.5926423048108473	0.0
16	6.0515324295571366E-5	0.0	0.0	1.6667130617486265	0.0
17	6.0515324295571366E-5	0.0	0.0	1.7461091672244162	0.0
18	6.0515324295571366E-5	0.0	0.0	1.8800295798905158	0.0
19	6.0515324295571366E-5	0.0	0.0	1.9318306974875248	0.0
20	1.815459728867141E-4	0.0	0.0	1.9988211614827223	0.0
21	1.815459728867141E-4	0.0	0.0	2.072407795826137	0.0
22	1.815459728867141E-4	0.0	0.0	2.1566451272455724	0.0
23	1.815459728867141E-4	0.0	0.0	2.249717696012161	0.0
24	1.815459728867141E-4	0.0	6.0515324295571366E-5	2.3184025890876345	0.0
25	1.815459728867141E-4	0.0	6.0515324295571366E-5	2.3795230666261618	0.0
26	1.815459728867141E-4	0.0	6.0515324295571366E-5	2.4370126247069543	0.0
27	1.815459728867141E-4	0.0	6.0515324295571366E-5	2.50170350637892	0.0
28	2.4206129718228547E-4	0.0	6.0515324295571366E-5	2.571356644643123	0.0
29	2.4206129718228547E-4	0.0	6.0515324295571366E-5	2.653899546982282	0.0
30	2.4206129718228547E-4	0.0	6.0515324295571366E-5	2.7630086766871975	0.0
31	2.4206129718228547E-4	0.0	6.0515324295571366E-5	2.8495455904298645	0.0
32	2.4206129718228547E-4	0.0	6.0515324295571366E-5	2.9291837572028365	0.0
33	2.4206129718228547E-4	0.0	6.0515324295571366E-5	3.0116056288934048	0.0
34	3.0257662147785685E-4	0.0	6.0515324295571366E-5	3.1049202589571756	0.0
35	3.0257662147785685E-4	0.0	6.0515324295571366E-5	3.223046171982131	0.0
36	3.0257662147785685E-4	0.0	6.0515324295571366E-5	3.3201127521522276	0.0
37	3.0257662147785685E-4	0.0	6.0515324295571366E-5	3.420568190482876	0.0
38	3.630919457734282E-4	0.0	6.0515324295571366E-5	3.5417803850469056	0.0
39	3.630919457734282E-4	0.0	6.0515324295571366E-5	3.748682278813464	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTCGAA	30	2.1660817E-6	45.000004	8
TATAGCG	140	0.0	45.000004	1
TACGTTG	30	2.1660817E-6	45.000004	1
GTCGACT	25	3.8915496E-5	45.0	34
CTATACG	40	6.8175723E-9	45.0	1
CGGTCGA	25	3.8915496E-5	45.0	31
TTCGATC	25	3.8915496E-5	45.0	25
ATCGCAC	45	3.8562575E-10	45.0	11
ATCGACG	20	7.034185E-4	45.0	1
TACGTAC	20	7.034185E-4	45.0	28
CCCGATA	20	7.034185E-4	45.0	16
CGTTTTT	5195	0.0	43.31088	1
ACTACGG	110	0.0	42.954544	2
TACGGGA	800	0.0	42.1875	4
CGTATGG	190	0.0	41.44737	2
CGACGGT	515	0.0	41.06796	28
ACGTAGG	225	0.0	41.0	2
CTATGCG	110	0.0	40.909092	1
AGGTACG	50	1.0822987E-9	40.5	1
TCGATAG	100	0.0	40.5	1
>>END_MODULE