FastQCFastQC Report
Thu 26 May 2016
SRR1545174_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545174_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1355666
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53380.393754803911878No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC52220.38519812402169856No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGCG650.045.0000041
TCGTAAG650.045.0000041
TATAGCG1300.045.0000041
ACAACGC406.8157533E-945.039
CTATGTC207.033735E-445.010
CTAGACG453.8562575E-1045.01
CTATGCG406.8157533E-945.01
TTAGGCG207.033735E-445.033
TAATACG207.033735E-445.01
TGATAGT207.033735E-445.032
CACGGAT351.2121382E-745.013
ACGTTAC207.033735E-445.033
CGTGCAT207.033735E-445.025
GACACGT207.033735E-445.09
GAATCTA750.045.09
ACCGAGT453.8562575E-1045.037
AAGCGTA351.2121382E-745.023
CTAAGCG800.045.01
ACGATAT207.033735E-445.033
CCGTAGA207.033735E-445.025