##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545174_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1355666
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.067327055484167	31.0	31.0	33.0	30.0	34.0
2	31.32503581265592	31.0	31.0	34.0	28.0	34.0
3	31.43062671779037	31.0	31.0	34.0	30.0	34.0
4	35.189931738348534	37.0	35.0	37.0	32.0	37.0
5	35.041867982231615	37.0	35.0	37.0	32.0	37.0
6	35.08763441732698	37.0	35.0	37.0	32.0	37.0
7	35.7435880224185	37.0	35.0	37.0	35.0	37.0
8	35.771805149646006	37.0	35.0	37.0	35.0	37.0
9	37.48125570752678	39.0	37.0	39.0	35.0	39.0
10	36.66231652929261	39.0	35.0	39.0	32.0	39.0
11	36.250931276582875	38.0	35.0	39.0	31.0	39.0
12	35.083116342816005	37.0	34.0	39.0	30.0	39.0
13	34.51558717265167	35.0	33.0	39.0	26.0	39.0
14	35.38065349429727	37.0	33.0	40.0	27.0	41.0
15	35.87623131361264	37.0	34.0	40.0	30.0	41.0
16	36.123484693132376	37.0	34.0	40.0	31.0	41.0
17	36.086822270382235	37.0	34.0	40.0	31.0	41.0
18	36.03623975226937	36.0	35.0	40.0	30.0	41.0
19	35.92333436111845	36.0	34.0	40.0	30.0	41.0
20	35.755796044158366	36.0	34.0	40.0	30.0	41.0
21	35.589300019326295	36.0	34.0	40.0	30.0	41.0
22	35.482919834236455	35.0	34.0	40.0	30.0	41.0
23	35.50095451239464	35.0	34.0	40.0	30.0	41.0
24	35.41667047783156	35.0	34.0	40.0	29.0	41.0
25	35.32984156864597	35.0	34.0	40.0	29.0	41.0
26	35.14131651896559	35.0	34.0	40.0	29.0	41.0
27	35.07607478538224	35.0	34.0	40.0	29.0	41.0
28	35.11041952811386	36.0	34.0	40.0	29.0	41.0
29	35.14527693399407	36.0	34.0	40.0	29.0	41.0
30	35.0742941107913	36.0	34.0	40.0	29.0	41.0
31	34.855519722409504	35.0	34.0	40.0	28.0	41.0
32	34.673746335749364	35.0	34.0	40.0	27.0	41.0
33	34.436075700061814	35.0	33.0	40.0	26.0	41.0
34	34.305657883283935	35.0	33.0	40.0	25.0	41.0
35	34.173436524925755	35.0	33.0	40.0	25.0	41.0
36	33.93383547274919	35.0	33.0	40.0	23.0	41.0
37	33.85242087652858	35.0	33.0	40.0	23.0	41.0
38	33.879156812961305	35.0	33.0	40.0	23.0	41.0
39	33.8565539004445	35.0	33.0	39.0	23.0	41.0
40	33.67969322827304	35.0	33.0	39.0	23.0	41.0
41	33.69948645167763	35.0	33.0	39.0	23.0	41.0
42	33.64635832129743	35.0	33.0	39.0	23.0	41.0
43	33.524965588869236	35.0	33.0	39.0	23.0	41.0
44	33.41344180646266	35.0	32.0	39.0	23.0	41.0
45	33.33686099673518	35.0	32.0	39.0	23.0	41.0
46	33.267711958550265	35.0	32.0	39.0	23.0	41.0
47	33.201789378799795	35.0	32.0	39.0	23.0	40.0
48	33.202150087115854	35.0	32.0	38.0	23.0	40.0
49	33.20422213140995	35.0	32.0	38.0	23.0	40.0
50	33.09464720661284	35.0	32.0	38.0	23.0	40.0
51	32.931738348531276	35.0	32.0	38.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	3.0
13	9.0
14	24.0
15	79.0
16	179.0
17	507.0
18	1065.0
19	2081.0
20	3455.0
21	5555.0
22	7995.0
23	11224.0
24	15467.0
25	20969.0
26	26111.0
27	30544.0
28	34025.0
29	39336.0
30	46140.0
31	56173.0
32	70160.0
33	88898.0
34	148316.0
35	213607.0
36	91844.0
37	105391.0
38	136328.0
39	200012.0
40	168.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.26657598553036	24.194750034300483	30.4443719913312	13.094301988837959
2	29.843707815936966	25.084939800806396	31.88668890419912	13.184663479057527
3	27.513635364462928	26.48159649943275	32.68142743124044	13.323340704863881
4	24.74746729651699	27.88076119044071	33.14533225735543	14.226439255686874
5	23.12265705564645	33.110072835049344	30.332913859313432	13.434356249990778
6	19.4346542584973	41.783890722346065	28.19802222671366	10.583432792442975
7	87.49360093120282	4.62304136859669	6.329656419796617	1.5537012804038752
8	89.5398276566647	3.1963625258728925	5.484536751677773	1.7792730657846403
9	86.06906125845157	4.90651827219979	6.892405651539539	2.1320148178091065
10	55.66636619934408	23.271882602351905	11.609054147555518	9.452697050748487
11	49.52023581029545	19.959267253143473	18.90480398564248	11.61569295091859
12	44.86031220079282	20.878962812374137	22.2266398950774	12.034085091755639
13	20.17134013835266	45.81452953751145	21.07938090945705	12.934749414678837
14	13.462903104451982	45.88962177999596	26.243927339034833	14.403547776517225
15	11.052353603321171	22.694749296655665	51.634620916951526	14.618276183071641
16	12.686384404418197	17.80977025314495	48.877157057859385	20.62668828457747
17	13.991351852152373	18.89728000849767	28.514693147132107	38.59667499221785
18	20.776356418173798	23.312453067348446	34.416589336901566	21.494601177576186
19	29.248280918751373	24.53768111024397	25.120494281039722	21.093543689964932
20	31.31671075323863	23.611272983168423	23.954204059111905	21.117812204481044
21	20.54281806875735	29.62418471806477	28.05477160303497	21.77822561014291
22	21.179184253348538	26.483145553550802	24.18434924236501	28.153320950735655
23	17.090787848924442	32.478058754885055	24.2717601533121	26.15939324287841
24	18.095091268793347	23.900429751871037	41.773121108001526	16.23135787133409
25	15.457863515054592	26.43254311902784	37.35411229609653	20.755481069821034
26	14.118595583277887	37.844867393591045	26.2009226461385	21.835614376992563
27	15.665067944464198	36.855021812157275	28.12580679901982	19.354103444358714
28	13.205022476037609	29.311128257255103	39.865866666273256	17.61798260043403
29	13.396810128748527	25.433403212885768	38.275430673927055	22.894355984438644
30	17.983190549884707	33.220203206394494	30.35002721909379	18.44657902462701
31	29.780639183987795	28.4612876623003	23.479160796243324	18.27891235746858
32	31.91995668549628	27.265712941093163	23.59836419885134	17.21596617455922
33	28.902546792499038	28.965246602039148	22.536524483169156	19.59568212229266
34	18.470626245697687	28.87931098072829	27.136256275513286	25.51380649806073
35	19.879749141750256	26.021158603962924	30.66588673021231	23.433205524074513
36	31.2141781235201	26.215454249055448	24.670014590614503	17.900353036809953
37	19.40418952750899	35.15652085395665	27.557820289068253	17.881469329466107
38	18.92449910228626	35.31363920021599	23.89312706817166	21.868734629326102
39	20.076331485778944	33.73839869112303	26.45422987668054	19.731039946417482
40	24.78250542537764	27.76908176497751	24.19770061357296	23.250712196071895
41	15.780656887463431	25.07077702029851	27.422978816316114	31.725587275921946
42	21.1051247136094	26.059958721395976	23.252556308117192	29.582360256877433
43	21.810239395249273	27.07407281734587	24.71051129112923	26.40517649627563
44	19.105738434098075	31.096154952621074	28.376237214771187	21.421869398509664
45	15.928407144532649	41.100610327322514	20.792215781763353	22.178766746381484
46	20.845178679704294	35.24525952557636	25.251573765219458	18.65798802949989
47	19.743727437289127	29.24599421981521	26.571441638279637	24.438836704616033
48	21.754325918035857	24.877514077951353	32.00537595543445	21.362784048578337
49	20.58700299336267	24.802200542021414	32.56325673137779	22.04753973323813
50	18.783313884098295	35.183149831890745	25.111642543222295	20.92189374078866
51	16.69511516848545	35.757701380723574	23.77768565413605	23.769497796654928
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1487.0
1	1591.5
2	1696.0
3	5099.0
4	8502.0
5	6481.0
6	4460.0
7	4993.0
8	5526.0
9	6329.0
10	7132.0
11	7876.5
12	8621.0
13	8424.0
14	8227.0
15	7968.5
16	7710.0
17	7105.0
18	6500.0
19	6104.0
20	5708.0
21	5602.0
22	5496.0
23	5388.0
24	5280.0
25	5567.0
26	8813.5
27	11773.0
28	13112.0
29	14451.0
30	16794.5
31	19138.0
32	23105.0
33	27072.0
34	33192.5
35	39313.0
36	42156.0
37	44999.0
38	54477.5
39	63956.0
40	80811.0
41	97666.0
42	124362.5
43	151059.0
44	151788.0
45	152517.0
46	143803.0
47	135089.0
48	117724.5
49	100360.0
50	94933.0
51	89506.0
52	79030.0
53	68554.0
54	64361.0
55	60168.0
56	54762.0
57	49356.0
58	44941.0
59	40526.0
60	37051.5
61	33577.0
62	28423.5
63	23270.0
64	20045.5
65	16821.0
66	14478.0
67	12135.0
68	10027.0
69	7919.0
70	6491.0
71	5063.0
72	4544.5
73	4026.0
74	3279.5
75	1983.0
76	1433.0
77	1047.5
78	662.0
79	487.5
80	313.0
81	219.0
82	125.0
83	89.5
84	54.0
85	35.5
86	17.0
87	14.5
88	12.0
89	7.5
90	3.0
91	1.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1355666.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.092870517250866
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.56649751606592	14.576792075865944
2	8.365577314948911	3.0271461432284372
3	2.9130571249504613	1.5811669611325134
4	1.3964177245574527	1.0106082031364831
5	0.8136273956377131	0.7360427559280593
6	0.536571269091069	0.5824868696965011
7	0.3795257233996588	0.4806696840005197
8	0.29415483249108115	0.42576842370278006
9	0.22382165959006112	0.36446186753272597
>10	2.3138833671538994	9.545337820395932
>50	0.7636666702850701	9.963372264241592
>100	1.4213817212335773	55.45577287117707
>500	0.009372643230611318	1.1320438803698363
>1k	0.0016300249096715337	0.3397458773442885
>5k	8.150124548357669E-4	0.7785843022473278
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5338	0.393754803911878	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5222	0.38519812402169856	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.376448181189171E-5	0.0	0.0	0.03798870813312424	0.0
2	7.376448181189171E-5	0.0	0.0	0.1380871099518613	0.0
3	7.376448181189171E-5	0.0	0.0	0.20071315501015738	0.0
4	7.376448181189171E-5	0.0	0.0	0.29520545621119065	0.0
5	7.376448181189171E-5	0.0	0.0	0.49938554186650697	0.0
6	7.376448181189171E-5	0.0	0.0	0.7198675780022513	0.0
7	7.376448181189171E-5	0.0	0.0	0.858987390699479	0.0
8	7.376448181189171E-5	0.0	0.0	1.2252280428955216	0.0
9	7.376448181189171E-5	0.0	0.0	1.3920095362722087	0.0
10	7.376448181189171E-5	0.0	0.0	1.6816088918656955	0.0
11	7.376448181189171E-5	0.0	0.0	1.9539473587151999	0.0
12	7.376448181189171E-5	0.0	0.0	2.1823221944048163	0.0
13	7.376448181189171E-5	0.0	0.0	2.2716509818790174	0.0
14	7.376448181189171E-5	0.0	0.0	2.3096396900121414	0.0
15	7.376448181189171E-5	0.0	0.0	2.3662170475618627	0.0
16	7.376448181189171E-5	0.0	0.0	2.498771821377832	0.0
17	7.376448181189171E-5	0.0	0.0	2.6570703993461517	0.0
18	7.376448181189171E-5	0.0	0.0	2.8924528608078983	0.0
19	7.376448181189171E-5	0.0	0.0	2.992698791590259	0.0
20	7.376448181189171E-5	0.0	0.0	3.094936363381541	0.0
21	7.376448181189171E-5	0.0	0.0	3.2169428162984097	0.0
22	7.376448181189171E-5	0.0	0.0	3.3323842303340205	0.0
23	7.376448181189171E-5	0.0	0.0	3.4815360125576653	0.0
24	7.376448181189171E-5	0.0	0.0	3.59166638390282	0.0
25	7.376448181189171E-5	0.0	0.0	3.67472519042301	0.0
26	7.376448181189171E-5	0.0	0.0	3.7551284755979717	0.0
27	7.376448181189171E-5	0.0	0.0	3.839441278308964	0.0
28	7.376448181189171E-5	0.0	0.0	3.922426320347342	0.0
29	7.376448181189171E-5	0.0	0.0	4.022598486647891	0.0
30	7.376448181189171E-5	0.0	0.0	4.148293163655355	0.0
31	1.4752896362378343E-4	0.0	0.0	4.255694249173469	0.0
32	1.4752896362378343E-4	0.0	0.0	4.346940913174779	0.0
33	1.4752896362378343E-4	0.0	0.0	4.4401054537031985	0.0
34	2.2129344543567517E-4	0.0	0.0	4.55289134639358	0.0
35	2.2129344543567517E-4	0.0	0.0	4.691568572199937	0.0
36	2.2129344543567517E-4	0.0	0.0	4.800592476317913	0.0
37	2.2129344543567517E-4	0.0	0.0	4.910575318699443	0.0
38	2.2129344543567517E-4	0.0	0.0	5.021074512453658	0.0
39	2.2129344543567517E-4	0.0	0.0	5.135261930298466	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTAGCG	65	0.0	45.000004	1
TCGTAAG	65	0.0	45.000004	1
TATAGCG	130	0.0	45.000004	1
ACAACGC	40	6.8157533E-9	45.0	39
CTATGTC	20	7.033735E-4	45.0	10
CTAGACG	45	3.8562575E-10	45.0	1
CTATGCG	40	6.8157533E-9	45.0	1
TTAGGCG	20	7.033735E-4	45.0	33
TAATACG	20	7.033735E-4	45.0	1
TGATAGT	20	7.033735E-4	45.0	32
CACGGAT	35	1.2121382E-7	45.0	13
ACGTTAC	20	7.033735E-4	45.0	33
CGTGCAT	20	7.033735E-4	45.0	25
GACACGT	20	7.033735E-4	45.0	9
GAATCTA	75	0.0	45.0	9
ACCGAGT	45	3.8562575E-10	45.0	37
AAGCGTA	35	1.2121382E-7	45.0	23
CTAAGCG	80	0.0	45.0	1
ACGATAT	20	7.033735E-4	45.0	33
CCGTAGA	20	7.033735E-4	45.0	25
>>END_MODULE