FastQCFastQC Report
Thu 26 May 2016
SRR1545173_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545173_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1754859
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99930.5694474598813922No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGA351.212411E-745.00000415
CTATGCG453.8562575E-1045.01
CTATACG253.89165E-545.01
TACGCTA253.89165E-545.027
TACGCGC207.034305E-445.034
TGTCGAC207.034305E-445.043
CGTTTTT75500.043.3013231
GCCGATT800.042.18759
CAATCGA1400.040.17857441
CGGTCTA2300.040.108731
TTACGCG403.4596087E-739.3751
ACGGGAT8850.039.1525425
CGTAAGG2250.039.02
TACGGGA6300.038.9285744
TATGGGC5150.038.8834954
ACGGGTA2200.038.8636365
CGAGGGT2500.038.74
GACCGAC356.2501713E-638.571439
AATGCGC356.2501713E-638.5714342
TATTCCG356.2501713E-638.571431