FastQCFastQC Report
Thu 26 May 2016
SRR1545172_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545172_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2339250
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128040.5473549214491824No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG23670.10118627765309395No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC207.0347864E-445.016
TGACGTA253.8920494E-545.021
CGTTTTT96050.043.1259771
CGGTCTA6800.042.3529431
ACGGTCT7000.041.14285730
TACGGGA9100.041.0439574
CGACGGT7250.040.0344828
CACGACG7450.039.86577226
GGCGATA8300.039.0361448
ACGGGAT8850.038.8983045
TTACGGG6250.038.883
ACGGGTA2550.038.823535
CGAACTA356.2510626E-638.57142636
ATATCGC356.2510626E-638.57142622
AGGGTAA11650.038.4334756
ACGACGG7800.038.07692727
TCACGAC7750.038.03225725
TAGGGTA16250.037.938465
GGGCGAT36150.037.9045647
ACTAATC20850.037.76978310
Produced by FastQC (version 0.11.5)