##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545172_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2339250
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.978298599978626	31.0	31.0	33.0	28.0	34.0
2	31.25281393609063	31.0	31.0	34.0	28.0	34.0
3	31.366069039221973	31.0	31.0	34.0	28.0	34.0
4	35.15718670514054	35.0	35.0	37.0	32.0	37.0
5	35.002472159880305	35.0	35.0	37.0	32.0	37.0
6	35.057248690819705	37.0	35.0	37.0	32.0	37.0
7	35.68774051512237	37.0	35.0	37.0	35.0	37.0
8	35.68958640589933	37.0	35.0	37.0	35.0	37.0
9	37.366052367211715	39.0	37.0	39.0	35.0	39.0
10	36.52431548573261	39.0	35.0	39.0	32.0	39.0
11	36.20926536282997	38.0	35.0	39.0	31.0	39.0
12	35.650234476862245	37.0	35.0	39.0	30.0	39.0
13	35.38957742866303	37.0	35.0	39.0	30.0	39.0
14	36.273697125146946	38.0	35.0	40.0	30.0	41.0
15	36.5314344341135	38.0	35.0	40.0	30.0	41.0
16	36.635050977877526	38.0	35.0	40.0	31.0	41.0
17	36.57645783905098	38.0	35.0	40.0	31.0	41.0
18	36.49274468312493	38.0	35.0	40.0	30.0	41.0
19	36.42836977663781	38.0	35.0	40.0	30.0	41.0
20	36.28307534466175	38.0	34.0	40.0	30.0	41.0
21	36.1779668697232	38.0	34.0	40.0	30.0	41.0
22	36.1153220049161	38.0	34.0	40.0	30.0	41.0
23	36.036683124933205	38.0	34.0	40.0	30.0	41.0
24	35.9534072886609	38.0	34.0	40.0	30.0	41.0
25	35.85036742545688	38.0	34.0	40.0	30.0	41.0
26	35.690299882440954	37.0	34.0	40.0	29.0	41.0
27	35.59008357379502	37.0	34.0	40.0	29.0	41.0
28	35.563188201346584	37.0	34.0	40.0	29.0	41.0
29	35.50865020840013	37.0	34.0	40.0	29.0	41.0
30	35.421353852730576	37.0	34.0	40.0	28.0	41.0
31	35.27686651704606	37.0	34.0	40.0	27.0	41.0
32	35.12438217377365	37.0	34.0	40.0	27.0	41.0
33	34.91521940792989	37.0	33.0	40.0	25.0	41.0
34	34.70856342844929	37.0	33.0	40.0	25.0	41.0
35	34.561329913433795	37.0	33.0	40.0	24.0	41.0
36	34.39173111039863	37.0	33.0	40.0	23.0	41.0
37	34.33020498022871	37.0	33.0	40.0	23.0	41.0
38	34.2643676392006	37.0	33.0	40.0	23.0	41.0
39	34.18269958319974	36.0	33.0	40.0	23.0	41.0
40	34.05265576573688	36.0	33.0	40.0	23.0	41.0
41	33.963398952655766	36.0	33.0	40.0	23.0	41.0
42	33.895404082505074	36.0	33.0	40.0	23.0	41.0
43	33.83698706850486	36.0	33.0	40.0	23.0	41.0
44	33.73887613551352	36.0	32.0	40.0	23.0	41.0
45	33.67297980121834	35.0	32.0	40.0	23.0	41.0
46	33.62449503045848	35.0	32.0	40.0	23.0	41.0
47	33.5128528374479	35.0	32.0	39.0	23.0	41.0
48	33.43879619536176	35.0	32.0	39.0	23.0	41.0
49	33.38171080474511	35.0	32.0	39.0	22.0	41.0
50	33.33829304264187	35.0	32.0	39.0	22.0	41.0
51	33.183840119696484	35.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	5.0
12	6.0
13	8.0
14	39.0
15	82.0
16	309.0
17	835.0
18	1869.0
19	3487.0
20	5893.0
21	9165.0
22	13793.0
23	19451.0
24	26607.0
25	35392.0
26	44871.0
27	51894.0
28	57501.0
29	65079.0
30	77305.0
31	91640.0
32	111170.0
33	138446.0
34	201371.0
35	252413.0
36	192471.0
37	234635.0
38	300444.0
39	402874.0
40	193.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.78326386662391	23.06418723949984	26.993096077802715	14.159452816073529
2	31.87273698835097	25.404039756332157	28.968216308645932	13.755006946670942
3	29.570588863952125	25.92758362723095	31.427936304371055	13.07389120444587
4	26.722325531687506	27.04153040504435	31.379972213316233	14.856171849951908
5	22.825392754087847	32.26204980228706	30.630501229026397	14.282056214598695
6	20.778155391685367	41.78356310783371	27.42323394250294	10.015047557977983
7	86.65456877204232	4.076007267286523	7.496291546435824	1.773132414235332
8	87.78873570588864	3.516896441167041	6.759944426632468	1.9344234263118523
9	83.27925617184995	5.094752591642621	8.509223041573154	3.116768194934274
10	43.087528053863416	29.364497167895692	14.783669979694347	12.764304798546542
11	36.49691140322753	23.651383990595278	24.11473762958213	15.736966976595063
12	34.44916105589398	21.526643154857325	27.323415624666026	16.700780164582664
13	24.993181575291228	29.458971892700653	27.973239286095968	17.57460724591215
14	19.595725125574436	31.69614192583093	30.234348616009406	18.47378433258523
15	17.83516084215026	22.968301806134445	39.44789996793844	19.748637383776853
16	21.196537351715293	20.802180185957038	36.835695201453454	21.16558726087421
17	21.280025649246554	20.997627444693812	29.93865555199316	27.783691354066477
18	23.16199636635674	23.466495671689643	31.84935342524313	21.522154536710485
19	27.809169605642836	25.178711125360692	25.95314737629582	21.05897189270065
20	28.3351501549642	24.6328096612162	26.267820882761566	20.76421930105803
21	24.43176231698194	26.32095757187133	29.067735385273057	20.179544725873676
22	23.756032916533076	23.595425884364644	27.80019236934915	24.848348829753125
23	21.779501977129424	27.24488618146842	27.24125253820669	23.73435930319547
24	21.394977022549963	25.166313989526557	33.51292080795127	19.925788179972212
25	21.838837234156248	25.54720530084429	30.569028534786792	22.044928930212677
26	20.239435716575827	28.285946350325958	27.71302767981191	23.76159025328631
27	20.461216201774075	27.96601474831677	29.07047130490542	22.50229774500374
28	19.94502511488725	26.372298813722345	32.35891845677033	21.32375761462007
29	20.336432617291866	24.844544191514377	32.38820134658545	22.430821844608314
30	22.357251255744362	27.081885219621675	30.14222507213851	20.418638452495458
31	27.05008015389548	25.362402479427164	26.91281393609063	20.674703430586728
32	27.84546328951587	24.9342310569627	26.171507961953616	21.04879769156781
33	27.211285668483487	25.541605215346795	25.82665384204339	21.420455274126322
34	21.527669124719463	26.41457732179117	28.00820775889708	24.049545794592284
35	22.672779737095222	25.965886502084	28.87459655872609	22.48673720209469
36	27.327348509137543	26.51349791599872	25.310676498877843	20.848477075985894
37	22.716084215026182	30.497894624345413	25.922453777920275	20.863567382708133
38	22.679106551245056	29.826696590787645	25.22000641231164	22.27419044565566
39	21.45888639521214	29.370481992091484	25.661087955541305	23.50954365715507
40	24.48831890563215	25.874788928075237	26.507042855616113	23.129849310676498
41	21.303537458587154	24.96402693170888	27.38334936411243	26.349086245591536
42	22.311082611948272	26.416501015282677	25.70016030779096	25.572256064978095
43	22.02205835203591	26.631527198888534	26.881094367852942	24.465320081222615
44	22.643838837234156	26.86348188521962	27.569178155391683	22.923501122154537
45	20.7021908731431	29.91227957678743	25.567639200598478	23.817890349470986
46	22.245206797050336	29.263439136475366	27.007929892059423	21.48342417441488
47	22.146670941541093	27.165672758362724	27.702938976167573	22.98471732392861
48	22.6972747675537	26.526792775462223	28.318948380891314	22.456984076092766
49	22.42449503045848	25.56943464785722	29.132542481564606	22.8735278401197
50	21.381724911830716	29.128139360906275	27.65544512129956	21.83469060596345
51	21.166057497061026	29.725852303088594	26.407481030244735	22.70060916960564
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1003.0
1	1913.0
2	2823.0
3	12228.0
4	21633.0
5	15765.5
6	9898.0
7	10133.0
8	10368.0
9	10824.5
10	11281.0
11	11468.0
12	11655.0
13	11159.0
14	10663.0
15	10260.0
16	9857.0
17	9452.5
18	9048.0
19	8807.0
20	8566.0
21	9159.5
22	9753.0
23	9974.0
24	10195.0
25	12620.5
26	18111.0
27	21176.0
28	22649.5
29	24123.0
30	28983.5
31	33844.0
32	41314.0
33	48784.0
34	56108.5
35	63433.0
36	70202.0
37	76971.0
38	82772.0
39	88573.0
40	109796.0
41	131019.0
42	145355.5
43	159692.0
44	165252.0
45	170812.0
46	170830.5
47	170849.0
48	170051.5
49	169254.0
50	170076.0
51	170898.0
52	159459.0
53	148020.0
54	137308.5
55	126597.0
56	123086.5
57	119576.0
58	118517.0
59	117458.0
60	104893.5
61	92329.0
62	83356.5
63	74384.0
64	68759.0
65	63134.0
66	53071.0
67	43008.0
68	35730.5
69	28453.0
70	24806.5
71	21160.0
72	17668.0
73	14176.0
74	12260.5
75	7593.0
76	4841.0
77	3655.0
78	2469.0
79	1794.0
80	1119.0
81	850.0
82	581.0
83	431.5
84	282.0
85	167.0
86	52.0
87	39.5
88	27.0
89	17.5
90	8.0
91	7.5
92	7.0
93	6.0
94	5.0
95	2.5
96	0.0
97	1.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2339250.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.51224089665015
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.69524166230018	15.160082718382503
2	7.076989812637702	2.761758600946517
3	2.875352036021797	1.6831368476859239
4	1.653759647144983	1.2907422648100824
5	1.1197425216762833	1.0924342912585072
6	0.8137829285609898	0.9527237123798084
7	0.6704896918931185	0.9157929470857485
8	0.5183792304650824	0.8091792336523853
9	0.40810354387035674	0.7166713192897531
>10	4.813742749864271	21.606494423453267
>50	1.3076472405902964	18.194626289979265
>100	1.0399517430862557	33.06505901918249
>500	0.004618097731113318	0.6207681914908647
>1k	0.0019791847419057076	0.5811200399088119
>5k	0.0	0.0
>10k+	2.1990941576730083E-4	0.5494101004941614
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12804	0.5473549214491824	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	2367	0.10118627765309395	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01957892486908197	0.0
2	0.0	0.0	0.0	0.07566527733247835	0.0
3	0.0	0.0	0.0	0.1093512878059207	0.0
4	0.0	0.0	0.0	0.16616436892166292	0.0
5	4.274874425563749E-5	0.0	0.0	0.29966869723201883	0.0
6	4.274874425563749E-5	0.0	0.0	0.4689964732285989	0.0
7	4.274874425563749E-5	0.0	0.0	0.5995938869295715	0.0
8	4.274874425563749E-5	0.0	0.0	0.9617612482633323	0.0
9	4.274874425563749E-5	0.0	0.0	1.1415624666025435	0.0
10	4.274874425563749E-5	0.0	0.0	1.4457197819814043	0.0
11	4.274874425563749E-5	0.0	0.0	1.6299241209789463	0.0
12	4.274874425563749E-5	0.0	0.0	1.8153681735599017	0.0
13	4.274874425563749E-5	0.0	0.0	1.8882547825157636	0.0
14	4.274874425563749E-5	0.0	0.0	1.92288126536283	0.0
15	4.274874425563749E-5	0.0	0.0	1.9631933311958962	0.0
16	4.274874425563749E-5	0.0	0.0	2.039841829646254	0.0
17	4.274874425563749E-5	0.0	0.0	2.1268782729507323	0.0
18	4.274874425563749E-5	0.0	0.0	2.2701293149513733	0.0
19	4.274874425563749E-5	0.0	0.0	2.330490541840333	0.0
20	4.274874425563749E-5	0.0	0.0	2.3990595276263758	0.0
21	8.549748851127498E-5	0.0	0.0	2.479127925617185	0.0
22	8.549748851127498E-5	0.0	0.0	2.566378112642941	0.0
23	8.549748851127498E-5	0.0	0.0	2.668718606390937	0.0
24	1.2824623276691248E-4	0.0	0.0	2.7431869188842577	0.0
25	1.2824623276691248E-4	0.0	0.0	2.8135086031847814	0.0
26	1.2824623276691248E-4	0.0	0.0	2.8808378753874107	0.0
27	1.7099497702254996E-4	0.0	0.0	2.95492144918243	0.0
28	1.7099497702254996E-4	0.0	0.0	3.031014213957465	0.0
29	1.7099497702254996E-4	0.0	0.0	3.118734637170033	0.0
30	2.1374372127818745E-4	0.0	0.0	3.255188628834028	0.0
31	2.1374372127818745E-4	0.0	0.0	3.351629795874746	0.0
32	2.1374372127818745E-4	0.0	0.0	3.438922731644758	0.0
33	2.5649246553382496E-4	0.0	0.0	3.5286950945815967	0.0
34	2.9924120978946244E-4	0.0	0.0	3.629368387303623	0.0
35	2.9924120978946244E-4	0.0	0.0	3.7633857005450464	0.0
36	3.4198995404509993E-4	0.0	0.0	3.8651704606177195	0.0
37	3.4198995404509993E-4	0.0	0.0	3.967938441808272	0.0
38	3.4198995404509993E-4	0.0	0.0	4.109821523992733	0.0
39	3.4198995404509993E-4	0.0	0.0	4.366955220690392	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGATC	20	7.0347864E-4	45.0	16
TGACGTA	25	3.8920494E-5	45.0	21
CGTTTTT	9605	0.0	43.125977	1
CGGTCTA	680	0.0	42.35294	31
ACGGTCT	700	0.0	41.142857	30
TACGGGA	910	0.0	41.043957	4
CGACGGT	725	0.0	40.03448	28
CACGACG	745	0.0	39.865772	26
GGCGATA	830	0.0	39.036144	8
ACGGGAT	885	0.0	38.898304	5
TTACGGG	625	0.0	38.88	3
ACGGGTA	255	0.0	38.82353	5
CGAACTA	35	6.2510626E-6	38.571426	36
ATATCGC	35	6.2510626E-6	38.571426	22
AGGGTAA	1165	0.0	38.433475	6
ACGACGG	780	0.0	38.076927	27
TCACGAC	775	0.0	38.032257	25
TAGGGTA	1625	0.0	37.93846	5
GGGCGAT	3615	0.0	37.904564	7
ACTAATC	2085	0.0	37.769783	10
>>END_MODULE