FastQCFastQC Report
Thu 26 May 2016
SRR1545171_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545171_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1689322
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131970.7812009788542386No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTCGA253.891586E-545.00000430
CGTTTTT86600.043.129331
ATATACG1100.042.9545441
TAGTACG850.042.352941
CTATGCG700.041.7857171
CGGTCTA3100.041.37096831
CGTAAGG2650.040.754722
TCGCGCA1500.040.534
CGTGAAT850.039.70588328
CACGCTA403.4594996E-739.37536
TAGGGAC16050.039.2523355
CTACGGG3200.038.6718753
TAAGGGC6650.038.5714264
ACCCGCT9000.038.2534
TATACGG2300.038.1521762
GCGTAAG1250.037.81
GGGCGAT31450.037.7742467
AGACGTG9000.037.7524
TCAGACG9250.037.702722
TAGGGAT19350.037.5581445
Produced by FastQC (version 0.11.5)