##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545171_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1689322
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.05102342833397	31.0	31.0	33.0	30.0	34.0
2	31.335383070841438	31.0	31.0	34.0	30.0	34.0
3	31.430518870884296	31.0	31.0	34.0	30.0	34.0
4	35.22828389140732	37.0	35.0	37.0	33.0	37.0
5	35.09841107852736	37.0	35.0	37.0	32.0	37.0
6	35.14616455595795	37.0	35.0	37.0	32.0	37.0
7	35.736434498573985	37.0	35.0	37.0	35.0	37.0
8	35.72522763570237	37.0	35.0	37.0	35.0	37.0
9	37.394023164322725	39.0	37.0	39.0	35.0	39.0
10	36.612143214851876	39.0	35.0	39.0	32.0	39.0
11	36.315884123926644	38.0	35.0	39.0	32.0	39.0
12	35.848060346103345	37.0	35.0	39.0	31.0	39.0
13	35.67288592701687	37.0	35.0	39.0	30.0	39.0
14	36.633029108719356	38.0	35.0	41.0	31.0	41.0
15	36.83073209251996	38.0	35.0	41.0	31.0	41.0
16	36.88982266258298	38.0	35.0	41.0	31.0	41.0
17	36.82197118133784	38.0	35.0	41.0	31.0	41.0
18	36.71395447404343	38.0	35.0	40.0	31.0	41.0
19	36.61821310561278	38.0	35.0	40.0	31.0	41.0
20	36.47507520768687	38.0	35.0	40.0	30.0	41.0
21	36.362709418334695	38.0	35.0	40.0	30.0	41.0
22	36.286642215042484	38.0	35.0	40.0	30.0	41.0
23	36.190772392711395	38.0	34.0	40.0	30.0	41.0
24	36.09492032898405	38.0	34.0	40.0	30.0	41.0
25	35.99408283323132	38.0	34.0	40.0	30.0	41.0
26	35.81187363924699	38.0	34.0	40.0	29.0	41.0
27	35.716269604018656	38.0	34.0	40.0	29.0	41.0
28	35.68155982104063	38.0	34.0	40.0	29.0	41.0
29	35.61697592288504	38.0	34.0	40.0	29.0	41.0
30	35.531719826060396	37.0	34.0	40.0	28.0	41.0
31	35.39374198643006	38.0	34.0	40.0	27.0	41.0
32	35.227676547159156	37.0	34.0	40.0	27.0	41.0
33	35.0061409251759	37.0	34.0	40.0	25.0	41.0
34	34.77817017714799	37.0	33.0	40.0	24.0	41.0
35	34.60074574296671	37.0	33.0	40.0	24.0	41.0
36	34.4255079848602	37.0	33.0	40.0	23.0	41.0
37	34.37410156263874	37.0	33.0	40.0	23.0	41.0
38	34.2888993335788	37.0	33.0	40.0	23.0	41.0
39	34.2168645172442	37.0	33.0	40.0	23.0	41.0
40	34.08355896625984	37.0	33.0	40.0	23.0	41.0
41	33.9785529342541	36.0	33.0	40.0	23.0	41.0
42	33.902253093252796	36.0	33.0	40.0	23.0	41.0
43	33.84503013635056	36.0	33.0	40.0	23.0	41.0
44	33.71463107684622	36.0	32.0	40.0	23.0	41.0
45	33.63895219502262	35.0	32.0	40.0	23.0	41.0
46	33.58409290827918	35.0	32.0	40.0	23.0	41.0
47	33.49652345733969	35.0	32.0	40.0	23.0	41.0
48	33.405502917738595	35.0	32.0	39.0	22.0	41.0
49	33.33502849071994	35.0	32.0	39.0	22.0	41.0
50	33.29230484182412	35.0	32.0	39.0	22.0	41.0
51	33.134841078255064	35.0	32.0	39.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	3.0
13	6.0
14	26.0
15	79.0
16	215.0
17	560.0
18	1249.0
19	2517.0
20	4179.0
21	6424.0
22	9314.0
23	13353.0
24	18546.0
25	25491.0
26	32913.0
27	37714.0
28	41507.0
29	46409.0
30	53475.0
31	63730.0
32	76715.0
33	96701.0
34	139999.0
35	174237.0
36	142691.0
37	173490.0
38	225741.0
39	301855.0
40	180.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.28008396267852	23.080857290676377	27.464746211793845	15.174312534851259
2	33.85488379361661	25.10569328997077	28.25654315755078	12.78287975886184
3	28.924029877074947	24.677651744309255	32.96955820145597	13.428760177159832
4	26.150787120513435	27.38080721141381	31.52909865614726	14.939307011925496
5	22.570238237588807	31.556210124535166	31.245493754299066	14.628057883576961
6	20.617146997434475	41.10631365719502	28.248610981210216	10.027928364160296
7	85.69177456991622	4.006400200790613	8.549998164944279	1.7518270643488927
8	86.35896531271126	3.4689656560442597	8.350391458821942	1.8216775724225458
9	81.43515564232277	5.421287356702867	9.95446693999131	3.189090060983045
10	41.480191461426536	29.71778026924411	16.223786820984987	12.578241448344366
11	34.37242870216572	23.43537821682308	25.03300140529751	17.15919167571369
12	31.433853344714624	21.91861587074578	28.99417636187772	17.653354422661874
13	23.979146663572724	27.697087944157477	30.09467703611271	18.229088356157085
14	19.66498985983726	30.560603603102308	31.100465157027497	18.673941380032936
15	18.705314913320255	23.602545873433247	38.55623735439425	19.13590185885225
16	21.13220570145893	20.87316686812816	36.483808297056456	21.51081913335646
17	21.889195783870687	21.1420321288659	30.16458673953219	26.80418534773122
18	23.78303248285407	22.9982205879045	32.15674690793111	21.062000021310322
19	26.91298639335781	24.71612871909559	27.2514653807859	21.1194195067607
20	28.136258214834115	23.746627345171614	27.415140511992387	20.701973928001884
21	24.87169408792403	25.13410705596683	29.299269174260445	20.694929681848695
22	24.13346893013884	22.530991723306748	28.737446147034134	24.59809319952028
23	22.36382406669658	25.879258069213567	28.425901041956475	23.33101682213338
24	21.50034155714541	24.034671897956695	33.98600148461927	20.47898506027862
25	22.724797285538223	24.597205269332903	30.57617197905432	22.10182546607456
26	21.52029038868848	27.56786450422122	27.876805014082574	23.035040093007726
27	21.179206806044082	26.97070185553731	29.886427809499907	21.963663528918705
28	20.517698816448256	25.598316957927498	32.45834719491015	21.4256370307141
29	21.259298108945483	24.611708129060062	31.413312559713308	22.71568120228115
30	22.2237086831285	26.6931940743091	30.432386484045075	20.65071075851732
31	26.36844840711244	25.420671725106285	27.195052216214556	21.015827651566724
32	26.367738062962538	25.506386585861073	27.222874028752365	20.903001322424025
33	26.106272220452944	25.913709760483794	26.750435973722002	21.229582045341267
34	22.170965629998307	26.3495058964484	28.765386350263594	22.714142123289697
35	23.452367281074892	25.659524945510686	29.2683100083939	21.619797765020525
36	26.896530087218423	26.73806414644455	25.741036936711886	20.62436882962514
37	22.887998853978107	29.760282527546554	26.238218646297153	21.113499972178186
38	23.00692230374079	28.612484771997288	26.586287279748916	21.794305644513006
39	22.774580571377157	28.472073411700077	26.06057341347594	22.692772603446826
40	23.989328263054645	25.956093628094585	27.484458261953616	22.570119846897157
41	21.751921776902215	25.230417883624316	27.561589797563755	25.456070541909714
42	22.563489968164742	26.589957391190076	26.505426437351794	24.341126203293392
43	22.600072691884673	26.401420214737037	27.456222081995023	23.542285011383264
44	22.578999148770926	26.57480338265884	28.216171931698042	22.63002553687219
45	20.850554246022963	29.437549502107945	26.389640340917836	23.322255910951256
46	22.591015803973427	28.27370980784007	27.555966239710365	21.579308148476134
47	22.440304453502648	27.506064563179784	27.806184966513193	22.247446016804375
48	22.38566714930605	26.474940834251846	28.469054449062998	22.670337567379104
49	22.598000854780796	25.947806279679064	29.70238947932958	21.751803386210565
50	21.568830572265085	28.118973173853178	28.0603697814863	22.251826472395432
51	21.67709885977925	28.564122174458156	27.22547862396867	22.533300341793925
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1044.0
1	1701.5
2	2359.0
3	12243.0
4	22127.0
5	15748.5
6	9370.0
7	9500.0
8	9630.0
9	9897.0
10	10164.0
11	10131.5
12	10099.0
13	9731.5
14	9364.0
15	8894.5
16	8425.0
17	7855.0
18	7285.0
19	7299.0
20	7313.0
21	7405.0
22	7497.0
23	7814.5
24	8132.0
25	9525.5
26	13132.5
27	15346.0
28	18791.5
29	22237.0
30	25526.0
31	28815.0
32	33160.0
33	37505.0
34	42778.5
35	48052.0
36	51153.5
37	54255.0
38	59488.5
39	64722.0
40	75431.0
41	86140.0
42	94779.5
43	103419.0
44	108181.0
45	112943.0
46	114245.5
47	115548.0
48	115716.0
49	115884.0
50	112937.0
51	109990.0
52	105355.5
53	100721.0
54	96034.5
55	91348.0
56	91133.0
57	90918.0
58	85673.0
59	80428.0
60	76420.0
61	72412.0
62	65524.5
63	58637.0
64	52672.0
65	46707.0
66	41097.5
67	35488.0
68	31005.5
69	26523.0
70	22426.0
71	18329.0
72	15506.0
73	12683.0
74	10214.5
75	5811.0
76	3876.0
77	3036.0
78	2196.0
79	1846.0
80	1496.0
81	1074.5
82	653.0
83	517.5
84	382.0
85	238.5
86	95.0
87	86.5
88	78.0
89	42.0
90	6.0
91	10.5
92	15.0
93	7.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1689322.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.505170208147526
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.63334332334114	16.480130917878565
2	6.4846177678890085	2.7890561766646162
3	2.7909031848894283	1.8005654407652454
4	1.6296864604329113	1.4018673887009294
5	1.1757546995895227	1.2642402468851024
6	0.8586683053988755	1.107948483596665
7	0.6746318991168376	1.0155651672847381
8	0.5639554812214868	0.9702366890788653
9	0.5045262046209206	0.9764929714359207
>10	6.592818667447586	33.49122697376296
>50	1.6304779780239416	24.413197762845687
>100	0.45621611374135324	12.858034892907089
>500	0.003574930358212734	0.5274524108985086
>1k	5.499892858788822E-4	0.12353941993126856
>5k	0.0	0.0
>10k+	2.749946429394411E-4	0.7804450573637871
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13197	0.7812009788542386	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.013555734193954734	0.0
2	0.0	0.0	0.0	0.052387881055239914	0.0
3	0.0	0.0	0.0	0.08275509346353152	0.0
4	0.0	0.0	0.0	0.1205809194457895	0.0
5	0.0	0.0	0.0	0.2104394544083366	0.0
6	0.0	0.0	0.0	0.34001806641954585	0.0
7	0.0	0.0	0.0	0.4306461408778196	0.0
8	0.0	0.0	0.0	0.6586666129962198	0.0
9	0.0	0.0	0.0	0.7894291319239316	0.0
10	0.0	0.0	0.0	0.9868456102507397	0.0
11	0.0	0.0	0.0	1.1168385896827249	0.0
12	5.919534582512984E-5	0.0	0.0	1.237241923091039	0.0
13	5.919534582512984E-5	0.0	0.0	1.2869068182383228	0.0
14	5.919534582512984E-5	0.0	0.0	1.3130711610930301	0.0
15	5.919534582512984E-5	0.0	0.0	1.3408929736308413	0.0
16	5.919534582512984E-5	0.0	0.0	1.3937544174526821	0.0
17	5.919534582512984E-5	0.0	0.0	1.457093437485571	0.0
18	5.919534582512984E-5	0.0	0.0	1.5519835768432544	0.0
19	5.919534582512984E-5	0.0	0.0	1.594249053762397	0.0
20	5.919534582512984E-5	0.0	0.0	1.644861074442883	0.0
21	5.919534582512984E-5	0.0	0.0	1.7019253878183083	0.0
22	5.919534582512984E-5	0.0	0.0	1.762482226597416	0.0
23	5.919534582512984E-5	0.0	0.0	1.833161469512621	0.0
24	5.919534582512984E-5	0.0	0.0	1.8895746340839699	0.0
25	5.919534582512984E-5	0.0	0.0	1.9382332083522265	0.0
26	5.919534582512984E-5	0.0	0.0	1.9873653453870843	0.0
27	1.7758603747538952E-4	0.0	0.0	2.0449032215291103	0.0
28	1.7758603747538952E-4	0.0	0.0	2.098889376921629	0.0
29	1.7758603747538952E-4	0.0	0.0	2.1599197784673376	0.0
30	1.7758603747538952E-4	0.0	0.0	2.2497783134298848	0.0
31	1.7758603747538952E-4	0.0	0.0	2.3135908962293747	0.0
32	1.7758603747538952E-4	0.0	0.0	2.3822574973865254	0.0
33	1.7758603747538952E-4	0.0	0.0	2.449385019552223	0.0
34	1.7758603747538952E-4	0.0	0.0	2.5203602391965534	0.0
35	1.7758603747538952E-4	0.0	0.0	2.610396360196576	0.0
36	1.7758603747538952E-4	0.0	0.0	2.682081923990808	0.0
37	1.7758603747538952E-4	0.0	0.0	2.7629427663879356	0.0
38	1.7758603747538952E-4	0.0	0.0	2.8610886497660006	0.0
39	1.7758603747538952E-4	0.0	0.0	3.02713159480549	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACTCGA	25	3.891586E-5	45.000004	30
CGTTTTT	8660	0.0	43.12933	1
ATATACG	110	0.0	42.954544	1
TAGTACG	85	0.0	42.35294	1
CTATGCG	70	0.0	41.785717	1
CGGTCTA	310	0.0	41.370968	31
CGTAAGG	265	0.0	40.75472	2
TCGCGCA	150	0.0	40.5	34
CGTGAAT	85	0.0	39.705883	28
CACGCTA	40	3.4594996E-7	39.375	36
TAGGGAC	1605	0.0	39.252335	5
CTACGGG	320	0.0	38.671875	3
TAAGGGC	665	0.0	38.571426	4
ACCCGCT	900	0.0	38.25	34
TATACGG	230	0.0	38.152176	2
GCGTAAG	125	0.0	37.8	1
GGGCGAT	3145	0.0	37.774246	7
AGACGTG	900	0.0	37.75	24
TCAGACG	925	0.0	37.7027	22
TAGGGAT	1935	0.0	37.558144	5
>>END_MODULE