FastQCFastQC Report
Thu 26 May 2016
SRR1545170_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545170_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1778162
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74410.41846580907701325No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC25350.14256293858489835No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACTCG351.2124292E-745.021
ACGCATC207.03433E-445.017
CGATTCG253.8916696E-545.017
CGCTAAC207.03433E-445.014
GTCGTAC351.2124292E-745.040
TTACGTA207.03433E-445.013
GATTCGT207.03433E-445.09
TGTAACG207.03433E-445.01
GCGTATT207.03433E-445.09
ATCGAAT253.8916696E-545.012
ACGAACT551.8189894E-1245.039
TTCGAAC302.1661745E-644.99999625
TCGAACT302.1661745E-644.99999626
CGGTCTA4150.043.9156631
GTACGAG1750.042.428571
CGTTTTT62450.042.3698961
ACGTAGG1750.041.1428572
TCGTAAG2150.040.8139531
TGTTATC6800.040.6985324
GTAGGGT7500.040.4999964
Produced by FastQC (version 0.11.5)