##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545170_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1778162
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.133216771025364	31.0	31.0	33.0	30.0	34.0
2	31.413780634160442	31.0	31.0	34.0	30.0	34.0
3	31.529218372679203	31.0	31.0	34.0	30.0	34.0
4	35.302372337278605	37.0	35.0	37.0	33.0	37.0
5	35.16419538827171	37.0	35.0	37.0	32.0	37.0
6	35.22683759972376	37.0	35.0	37.0	32.0	37.0
7	35.780356907863286	37.0	35.0	37.0	35.0	37.0
8	35.78115492289229	37.0	35.0	37.0	35.0	37.0
9	37.474399970306415	39.0	37.0	39.0	35.0	39.0
10	36.70986445554455	39.0	37.0	39.0	32.0	39.0
11	36.421586447129116	38.0	35.0	39.0	32.0	39.0
12	35.80041413549497	37.0	35.0	39.0	31.0	39.0
13	35.54170204964452	37.0	35.0	39.0	30.0	39.0
14	36.550977919897065	38.0	35.0	40.0	31.0	41.0
15	36.78441165653073	38.0	35.0	41.0	31.0	41.0
16	36.88739777365617	38.0	35.0	41.0	31.0	41.0
17	36.795128340387436	38.0	35.0	40.0	31.0	41.0
18	36.716057367101534	38.0	35.0	40.0	31.0	41.0
19	36.66017044566243	38.0	35.0	40.0	31.0	41.0
20	36.523281905698134	38.0	35.0	40.0	31.0	41.0
21	36.40413921791153	38.0	35.0	40.0	31.0	41.0
22	36.308151338292014	38.0	35.0	40.0	30.0	41.0
23	36.235859837292665	38.0	35.0	40.0	30.0	41.0
24	36.17283464611211	38.0	34.0	40.0	30.0	41.0
25	36.064747194012696	38.0	34.0	40.0	30.0	41.0
26	35.89180569599395	38.0	34.0	40.0	30.0	41.0
27	35.80480462410062	37.0	34.0	40.0	30.0	41.0
28	35.79411268489598	37.0	34.0	40.0	30.0	41.0
29	35.76960985556997	37.0	34.0	40.0	30.0	41.0
30	35.70498301054685	37.0	34.0	40.0	29.0	41.0
31	35.60233432049498	37.0	34.0	40.0	29.0	41.0
32	35.46047547973694	37.0	34.0	40.0	29.0	41.0
33	35.24806626167919	37.0	34.0	40.0	27.0	41.0
34	35.09211871584254	37.0	34.0	40.0	27.0	41.0
35	34.94371491461408	37.0	34.0	40.0	26.0	41.0
36	34.791610100766974	37.0	33.0	40.0	25.0	41.0
37	34.72884191654079	37.0	33.0	40.0	25.0	41.0
38	34.680950891988466	37.0	33.0	40.0	25.0	41.0
39	34.605304803499344	37.0	33.0	40.0	25.0	41.0
40	34.4600227650799	36.0	33.0	40.0	24.0	41.0
41	34.380415282747016	36.0	33.0	40.0	24.0	41.0
42	34.33479008099375	36.0	33.0	40.0	24.0	41.0
43	34.29008380563751	36.0	33.0	40.0	24.0	41.0
44	34.15680517298199	36.0	33.0	40.0	23.0	41.0
45	34.08423416989003	35.0	33.0	40.0	23.0	41.0
46	33.990854039170785	35.0	33.0	40.0	23.0	41.0
47	33.88353254652838	35.0	33.0	40.0	23.0	41.0
48	33.83283694061621	35.0	33.0	39.0	23.0	41.0
49	33.805066130082636	35.0	33.0	39.0	24.0	41.0
50	33.7334691664764	35.0	33.0	39.0	24.0	41.0
51	33.55669280976649	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	3.0
13	2.0
14	9.0
15	45.0
16	151.0
17	470.0
18	1041.0
19	1946.0
20	3464.0
21	5506.0
22	8380.0
23	11766.0
24	16455.0
25	22449.0
26	29037.0
27	34261.0
28	39212.0
29	45032.0
30	53812.0
31	65546.0
32	81556.0
33	103659.0
34	159140.0
35	213110.0
36	145034.0
37	177056.0
38	233456.0
39	326331.0
40	232.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.37181876566927	23.816333944826173	29.001913211507162	13.809934077997394
2	30.160469068622543	26.08091951127063	30.75597161563457	13.002639804472258
3	29.42425943193027	25.415625797874437	32.320283528722356	12.839831241472938
4	27.446543115869083	27.256234246373502	30.765925714305	14.53129692345242
5	23.305244404053173	32.275574441473836	30.279918252667642	14.139262901805347
6	20.393136283420745	42.44326444947086	26.633400106402007	10.530199160706392
7	87.23845183959617	4.498127842120122	6.578421988547725	1.6849983297359858
8	88.58039931119886	3.533480076618441	6.240826201437214	1.6452944107454774
9	83.89618043800283	5.88478440097134	7.447296703000064	2.771738458025759
10	43.59928960353444	30.83588559422595	13.428304057785512	12.136520744454105
11	38.20782358412788	22.734374033412028	23.14378554934815	15.914016833111944
12	35.69219227494458	21.34231864138363	26.598701355669508	16.366787728002286
13	23.328358158592973	33.04811372642087	25.716498271811005	17.907029843175142
14	18.083560440499795	35.7008529031663	28.169480621000787	18.046106035333114
15	16.746786850691894	24.233000142844126	40.10708810558318	18.9131249008808
16	19.141338078307825	20.492171129514634	38.82452779892946	21.541962993248085
17	19.468023723372784	21.353509972657157	28.61319722275023	30.56526908121982
18	22.57285894086141	23.955410136984145	31.95783061385858	21.51390030829587
19	28.087542079967964	24.841662345725528	24.850210498256065	22.22058507605044
20	29.78541887634535	23.51321195706578	25.557513882312183	21.143855284276686
21	24.08886254458255	27.41386892757803	27.101074030375184	21.396194497464236
22	23.965307997808974	24.295480389300863	25.24696849893317	26.492243113957
23	21.580373441789895	28.146648055688967	25.45898517682866	24.813993325692486
24	20.94685411115523	24.007317668468904	35.149497064946836	19.896331155429035
25	21.22691858222142	25.42468009101533	31.23292478413103	22.115476542632223
26	19.867931043403246	31.222633258387035	25.9682751065426	22.941160591667124
27	20.019042134518678	31.278589914754672	27.391261313648585	21.311106637078062
28	19.346325025503862	27.795948850554673	33.45988723187201	19.39783889206945
29	20.068700152179613	24.820404440090385	32.54979017659808	22.561105231131922
30	20.742710731643125	29.580488166994908	28.948543496036923	20.728257605325048
31	27.119351330193766	27.383950393721157	25.0475490984511	20.449149177633984
32	28.349947867517134	26.84395460031201	25.00233387059222	19.803763661578643
33	27.05670237020024	27.460546339422393	24.30228516861793	21.180466121759434
34	21.110731193220865	27.74218547016526	26.805375438233416	24.341707898380463
35	21.87832154775549	25.48755400239123	29.490901278961086	23.143223170892192
36	27.707599195123954	26.738733591202603	25.14034154368387	20.413325669989575
37	21.855995123054033	31.62411523809417	26.362052501403134	20.15783713744867
38	22.17328904790452	30.647263860098235	24.727049616401654	22.452397475595586
39	22.552275889373412	30.032415494201313	25.3492651400716	22.066043476353673
40	24.675029609225707	26.299234827872827	26.20632990694886	22.819405655952608
41	19.479946146639058	26.015796086070896	28.015613875451166	26.488643891838876
42	22.008343446772567	26.89164429337709	24.63425717116888	26.46575508868146
43	22.329911447888325	26.5821111912188	25.488959948531125	25.599017412361754
44	21.636836238767895	28.289098518582673	27.158774059956293	22.91529118269314
45	20.174764728972953	32.59669253982483	24.037123726634583	23.19141900456764
46	21.475152432680485	30.982722609076113	26.618722028701548	20.923402929541854
47	22.4201169522237	27.640957348093142	26.48347001004408	23.455455689639077
48	21.41925201415844	26.524579875174474	29.321512888027073	22.734655222640008
49	21.508838902192267	26.427232164448462	29.76196769473198	22.301961238627303
50	20.993868950073168	29.901831216728286	26.96689053078403	22.137409302414515
51	21.002248389066914	30.23155370545541	25.554195849422044	23.212002056055635
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1151.0
1	1826.0
2	2501.0
3	8235.5
4	13970.0
5	10557.5
6	7145.0
7	7407.5
8	7670.0
9	8102.5
10	8535.0
11	8740.5
12	8946.0
13	8734.5
14	8523.0
15	7973.0
16	7423.0
17	6984.0
18	6545.0
19	6967.5
20	7390.0
21	7867.5
22	8345.0
23	7514.5
24	6684.0
25	7810.5
26	11769.0
27	14601.0
28	20076.0
29	25551.0
30	29719.5
31	33888.0
32	36757.0
33	39626.0
34	45560.0
35	51494.0
36	54650.5
37	57807.0
38	63758.5
39	69710.0
40	86263.5
41	102817.0
42	119583.5
43	136350.0
44	137523.5
45	138697.0
46	135711.5
47	132726.0
48	129297.5
49	125869.0
50	121165.5
51	116462.0
52	107809.5
53	99157.0
54	94813.0
55	90469.0
56	88267.5
57	86066.0
58	83494.5
59	80923.0
60	76217.0
61	71511.0
62	65250.0
63	58989.0
64	51968.0
65	44947.0
66	37970.0
67	30993.0
68	26778.0
69	22563.0
70	20098.5
71	17634.0
72	14471.0
73	11308.0
74	9139.0
75	5041.0
76	3112.0
77	2506.0
78	1900.0
79	1562.0
80	1224.0
81	849.5
82	475.0
83	433.5
84	392.0
85	236.0
86	80.0
87	69.0
88	58.0
89	40.0
90	22.0
91	12.5
92	3.0
93	2.0
94	1.0
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1778162.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.88475210330865
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.9733024940783	14.124179378861971
2	7.0811570303875095	2.5328947618616366
3	2.5096831199045257	1.3465518137205206
4	1.4103790096881892	1.0089711583993286
5	0.896139358522423	0.8013615138595784
6	0.6243920202579916	0.670025789855895
7	0.5147401326740219	0.6444199769349359
8	0.4218246506414091	0.6035383446229103
9	0.3423087219608063	0.5509895971562483
>10	4.470617474165028	18.874236898377074
>50	1.4076447096253157	18.00157493635298
>100	1.3399385486663875	39.14560774197026
>500	0.005038546833993164	0.6204291140831577
>1k	0.002519273416996582	0.6561364541638333
>5k	3.1490917712457276E-4	0.4190825197796638
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7441	0.41846580907701325	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2535	0.14256293858489835	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02699416588589791	0.0
2	0.0	0.0	0.0	0.1081453770803785	0.0
3	0.0	0.0	0.0	0.1487491016004166	0.0
4	0.0	0.0	0.0	0.20695527179188397	0.0
5	0.0	0.0	0.0	0.3329280459260742	0.0
6	0.0	0.0	0.0	0.4592382471338382	0.0
7	0.0	0.0	0.0	0.5357217171438823	0.0
8	0.0	0.0	0.0	0.7191695694767968	0.0
9	0.0	0.0	0.0	0.8058883273852439	0.0
10	0.0	0.0	0.0	0.9641416248913204	0.0
11	0.0	0.0	0.0	1.1213264033310801	0.0
12	0.0	0.0	0.0	1.2603463576434544	0.0
13	0.0	0.0	0.0	1.3201834253571947	0.0
14	0.0	0.0	0.0	1.3470088777063056	0.0
15	0.0	0.0	0.0	1.3871626994615789	0.0
16	0.0	0.0	0.0	1.474162646598004	0.0
17	0.0	0.0	0.0	1.576740476964416	0.0
18	0.0	0.0	0.0	1.7287513736093787	0.0
19	0.0	0.0	0.0	1.7942122258826811	0.0
20	0.0	0.0	0.0	1.8642283436492288	0.0
21	0.0	0.0	0.0	1.9471791659027693	0.0
22	0.0	0.0	0.0	2.0297363232371404	0.0
23	0.0	0.0	0.0	2.1284337422574544	0.0
24	0.0	0.0	0.0	2.2046360230395208	0.0
25	0.0	0.0	0.0	2.2658790368931516	0.0
26	0.0	0.0	0.0	2.328359283349886	0.0
27	0.0	0.0	0.0	2.392751616556872	0.0
28	0.0	0.0	0.0	2.4560754306975405	0.0
29	0.0	0.0	0.0	2.527103829684809	0.0
30	0.0	0.0	0.0	2.6204586533735394	0.0
31	5.623784559562065E-5	0.0	0.0	2.6993603507441954	0.0
32	5.623784559562065E-5	0.0	0.0	2.779443042872359	0.0
33	5.623784559562065E-5	0.0	0.0	2.8555890858088295	0.0
34	5.623784559562065E-5	0.0	0.0	2.940170805584643	0.0
35	5.623784559562065E-5	0.0	0.0	3.03549395386922	0.0
36	5.623784559562065E-5	0.0	0.0	3.112877229408794	0.0
37	5.623784559562065E-5	0.0	0.0	3.1994272737804543	0.0
38	5.623784559562065E-5	0.0	0.0	3.286202269534497	0.0
39	5.623784559562065E-5	0.0	0.0	3.3786010498481014	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAACTCG	35	1.2124292E-7	45.0	21
ACGCATC	20	7.03433E-4	45.0	17
CGATTCG	25	3.8916696E-5	45.0	17
CGCTAAC	20	7.03433E-4	45.0	14
GTCGTAC	35	1.2124292E-7	45.0	40
TTACGTA	20	7.03433E-4	45.0	13
GATTCGT	20	7.03433E-4	45.0	9
TGTAACG	20	7.03433E-4	45.0	1
GCGTATT	20	7.03433E-4	45.0	9
ATCGAAT	25	3.8916696E-5	45.0	12
ACGAACT	55	1.8189894E-12	45.0	39
TTCGAAC	30	2.1661745E-6	44.999996	25
TCGAACT	30	2.1661745E-6	44.999996	26
CGGTCTA	415	0.0	43.91566	31
GTACGAG	175	0.0	42.42857	1
CGTTTTT	6245	0.0	42.369896	1
ACGTAGG	175	0.0	41.142857	2
TCGTAAG	215	0.0	40.813953	1
TGTTATC	680	0.0	40.69853	24
GTAGGGT	750	0.0	40.499996	4
>>END_MODULE