FastQCFastQC Report
Thu 26 May 2016
SRR1545169_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545169_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1278999
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77240.6039097763172606No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC48020.3754498635260856No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15310.11970298647614268No Hit
GGTAGAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA12860.10054738119419952No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAG551.8189894E-1245.0000041
ACACGTG302.1656942E-645.00000442
GTAGCAC302.1656942E-645.00000424
ACAATCG302.1656942E-645.00000445
TAGTCCG600.045.0000041
AGTAAGA302.1656942E-645.00000416
TGATCCA207.033585E-445.031
TGTTTCG207.033585E-445.09
CGGCGTA207.033585E-445.014
GCCTTAC207.033585E-445.023
TCCGCAA1050.045.032
ACGCATT207.033585E-445.015
TAGCGTC207.033585E-445.032
CGACAGG351.2120654E-745.02
TCTAGCG800.045.01
ATGTACC207.033585E-445.040
TAGCACG900.045.01
CTAACGG800.045.02
TACGCCT453.8562575E-1045.034
CATAGGT207.033585E-445.031