##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545169_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1278999
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.05182724927854	31.0	31.0	33.0	28.0	34.0
2	31.32317382578094	31.0	31.0	34.0	28.0	34.0
3	31.39798154650629	31.0	31.0	34.0	28.0	34.0
4	35.14996258793009	37.0	35.0	37.0	32.0	37.0
5	34.98946207151061	35.0	35.0	37.0	32.0	37.0
6	35.05385696157698	37.0	35.0	37.0	32.0	37.0
7	35.724323474842436	37.0	35.0	37.0	35.0	37.0
8	35.7494274819605	37.0	35.0	37.0	35.0	37.0
9	37.46075876525314	39.0	37.0	39.0	35.0	39.0
10	36.67947590263949	39.0	35.0	39.0	32.0	39.0
11	36.20377341968211	38.0	35.0	39.0	31.0	39.0
12	34.85993421417843	35.0	34.0	39.0	30.0	39.0
13	34.236740607303055	35.0	33.0	39.0	25.0	39.0
14	35.05114312051847	36.0	33.0	40.0	25.0	41.0
15	35.608364040941396	36.0	33.0	40.0	30.0	41.0
16	35.8933017148567	36.0	34.0	40.0	31.0	41.0
17	35.85171997788896	36.0	34.0	40.0	31.0	41.0
18	35.769318818857556	36.0	34.0	40.0	30.0	41.0
19	35.64798877872461	36.0	34.0	40.0	30.0	41.0
20	35.45890731736303	35.0	34.0	40.0	30.0	41.0
21	35.26191810939649	35.0	34.0	40.0	29.0	41.0
22	35.139060312009626	35.0	34.0	40.0	29.0	41.0
23	35.145867197706956	35.0	34.0	40.0	29.0	41.0
24	35.05272560807319	35.0	34.0	40.0	29.0	41.0
25	34.98194291004137	35.0	34.0	39.0	29.0	41.0
26	34.807433782199986	35.0	34.0	39.0	29.0	41.0
27	34.696824626133406	35.0	34.0	39.0	29.0	41.0
28	34.70054550472674	35.0	34.0	39.0	28.0	41.0
29	34.771045168917254	35.0	34.0	39.0	29.0	41.0
30	34.70205997033618	35.0	34.0	39.0	29.0	41.0
31	34.43157891444794	35.0	33.0	39.0	27.0	41.0
32	34.19304237141702	35.0	33.0	39.0	25.0	41.0
33	33.95248237097918	35.0	33.0	39.0	24.0	41.0
34	33.80234620980939	35.0	33.0	39.0	24.0	41.0
35	33.646696361764164	35.0	33.0	39.0	23.0	41.0
36	33.37884236031459	35.0	33.0	39.0	23.0	41.0
37	33.266919677028675	35.0	32.0	39.0	23.0	41.0
38	33.27130904715328	35.0	32.0	39.0	23.0	41.0
39	33.22205959504269	35.0	32.0	39.0	23.0	41.0
40	33.01390775129613	35.0	32.0	39.0	22.0	41.0
41	33.041941393230175	35.0	32.0	38.0	22.0	41.0
42	32.969578553227954	35.0	32.0	38.0	22.0	41.0
43	32.87837207065838	35.0	32.0	38.0	22.0	41.0
44	32.75660027881179	35.0	32.0	38.0	21.0	40.0
45	32.679114682654166	35.0	32.0	38.0	22.0	40.0
46	32.615740121767104	35.0	32.0	38.0	21.0	40.0
47	32.55569863619909	35.0	32.0	38.0	21.0	40.0
48	32.55891130485637	35.0	32.0	38.0	21.0	40.0
49	32.52464466352202	35.0	31.0	38.0	20.0	40.0
50	32.41794481465583	35.0	31.0	38.0	20.0	40.0
51	32.24513232613943	35.0	31.0	37.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	2.0
13	3.0
14	21.0
15	67.0
16	213.0
17	569.0
18	1307.0
19	2460.0
20	4051.0
21	6212.0
22	8982.0
23	12639.0
24	17139.0
25	22695.0
26	27906.0
27	31696.0
28	34917.0
29	39313.0
30	45815.0
31	55922.0
32	68385.0
33	87698.0
34	150118.0
35	218608.0
36	82404.0
37	89958.0
38	113653.0
39	156097.0
40	148.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.747249215988443	23.500331118319874	30.29650531392128	14.455914351770408
2	30.54357352898634	27.32605733077196	29.5258244924351	12.604544647806604
3	26.5092466843211	26.408152000118847	33.88689123290949	13.195710082650573
4	24.869839616762796	28.61917796651913	32.97883735640138	13.532145060316699
5	22.45427869763776	32.332707062319834	32.14529487513282	13.067719364909589
6	19.50720837154681	40.248585026258816	29.312454505437458	10.931752096756917
7	85.80069257286362	5.5450395191864885	7.160990743542412	1.493277164407478
8	87.69991219696028	3.8111835896666064	6.567010607514158	1.921893605858957
9	84.81359250476349	5.112904701254653	7.927058582532121	2.1464442114497353
10	58.82123441847883	20.052556726002134	12.10704621348414	9.019162642034905
11	54.26814250832096	18.1753074083717	17.572023121206506	9.98452696210083
12	46.7419442861175	21.997906175063466	20.34137634196743	10.9187731968516
13	20.355058917168815	48.8624306977566	20.48273689033377	10.299773494740808
14	12.399696950505826	50.37111053253365	26.123398063641957	11.105794453318572
15	10.123698298434949	22.63926711436053	55.26055923421364	11.976475352990894
16	11.379524143490338	17.686644008322133	52.33647563446101	18.597356213726517
17	13.23284850105434	17.76686299207427	28.135205735110034	40.86508277176135
18	19.73316632772973	23.21706271857914	36.17969990594207	20.870071047749057
19	29.172892238383298	24.37914337696902	25.614484452294334	20.83347993235335
20	32.39728881727038	23.434107454345156	23.82840017857715	20.34020354980731
21	20.309007278348147	31.49634987986699	28.1871213347313	20.007521507053564
22	21.435044124350373	26.177815619871474	24.000722439970634	28.38641781580752
23	16.688519693916884	33.05186321490478	23.263036171255802	26.99658091992253
24	17.789380601548554	24.49595347611687	42.65757830928719	15.05708761304739
25	14.288439631305419	25.52973067218974	39.514026203304304	20.667803493200545
26	13.157867988950734	38.760859078075896	28.473908110952394	19.60736482202097
27	15.090942213402824	38.909725496267	28.362961972605138	17.636370317725035
28	12.148484869808343	31.59658451648516	41.47657660404738	14.778354009659116
29	12.56162045474625	24.73176288644479	40.09706027917145	22.609556379637514
30	17.15161622487586	33.89603901175842	30.815974054709972	18.136370708655754
31	29.760773855178936	29.367106620098998	23.600018451929987	17.272101072792083
32	31.27922695795696	28.13770769171829	25.86905853718416	14.714006813140589
33	29.510578194353553	29.42668446183304	23.476875275117496	17.58586206869591
34	18.82018672414912	30.28563743990417	26.632937164141644	24.26123867180506
35	18.863345475641495	27.546620443018327	30.846544837017078	22.7434892443231
36	32.45772670658852	24.529886262616312	25.704789448623494	17.30759758217168
37	19.360531165387933	35.39173994663014	28.159365253608488	17.088363634373444
38	20.335825125742865	35.821216435665704	22.72863387696159	21.11432456162984
39	18.898685612733082	34.85632123246383	26.023319799311807	20.221673355491287
40	24.00275527971484	27.445603944960084	23.6169066590357	24.934734116289377
41	16.609473502324864	25.33840917780233	26.222459908100003	31.8296574117728
42	22.199391868171904	26.331216834415038	23.349510046528575	28.119881250884482
43	22.575467220850058	26.772812175771833	24.561395278651506	26.090325324726603
44	19.678826957644223	32.73591300696873	27.428168434846313	20.157091600540735
45	15.485547682210854	42.03208915722374	20.63864006148558	21.843723099079824
46	21.552010595786236	35.160152588078645	25.567260021313544	17.720576794821575
47	22.216123702989606	29.31566013734178	25.356391990924156	23.111824168744462
48	23.164756188237835	25.051544215437225	30.44224428635206	21.341455309972876
49	21.2056459778311	23.999158717090474	32.83153466109043	21.963660643987996
50	19.862720768350876	36.3120690477475	24.450761884880283	19.374448299021342
51	17.837308707825418	37.865549543041084	21.453339682048227	22.843802067085274
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1870.0
1	2040.5
2	2211.0
3	6763.0
4	11315.0
5	8153.5
6	4992.0
7	5785.5
8	6579.0
9	7844.0
10	9109.0
11	10084.5
12	11060.0
13	11419.0
14	11778.0
15	11280.5
16	10783.0
17	9996.5
18	9210.0
19	8652.0
20	8094.0
21	8445.0
22	8796.0
23	8019.5
24	7243.0
25	7219.5
26	7681.5
27	8167.0
28	8761.0
29	9355.0
30	11683.0
31	14011.0
32	16905.5
33	19800.0
34	23823.0
35	27846.0
36	30076.0
37	32306.0
38	40877.0
39	49448.0
40	76023.5
41	102599.0
42	119750.0
43	136901.0
44	146103.0
45	155305.0
46	140615.0
47	125925.0
48	117347.5
49	108770.0
50	101981.0
51	95192.0
52	82468.5
53	69745.0
54	60092.0
55	50439.0
56	47170.5
57	43902.0
58	37556.5
59	31211.0
60	29448.5
61	27686.0
62	23033.5
63	18381.0
64	16298.5
65	14216.0
66	11934.5
67	9653.0
68	7876.5
69	6100.0
70	5011.0
71	3922.0
72	3732.0
73	3542.0
74	2798.5
75	1636.0
76	1217.0
77	850.5
78	484.0
79	385.0
80	286.0
81	245.5
82	205.0
83	125.0
84	45.0
85	34.0
86	23.0
87	17.0
88	11.0
89	8.0
90	5.0
91	6.0
92	7.0
93	4.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1278999.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.678857934264148
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.59914697973424	15.860991630370652
2	8.236822978971027	3.241825384657064
3	3.0720790940413996	1.813650241633908
4	1.6208016903911997	1.275821048192944
5	0.9179140404995489	0.9031749999428504
6	0.5815226420647442	0.6866220875250023
7	0.4179317514485746	0.5757093694082285
8	0.3029387115372235	0.47691902937040387
9	0.23425263751481687	0.4148841936942681
>10	2.1809326631028645	9.448909701567636
>50	0.6136110945148727	8.773262075796657
>100	1.1989506847235236	52.83296315703884
>500	0.021104080468398127	2.3988127389748897
>1k	0.001592760790067783	0.691207237791053
>5k	3.9819019751694576E-4	0.6052471040356073
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7724	0.6039097763172606	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4802	0.3754498635260856	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1531	0.11970298647614268	No Hit
GGTAGAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	1286	0.10054738119419952	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03596562624364835	0.0
2	0.0	0.0	0.0	0.18553571973082075	0.0
3	0.0	0.0	0.0	0.2803755124124413	0.0
4	0.0	0.0	0.0	0.42916374445953437	0.0
5	0.0	0.0	0.0	0.7337769615144344	0.0
6	0.0	0.0	0.0	1.0348718020889773	0.0
7	0.0	0.0	0.0	1.2236131537241233	0.0
8	0.0	0.0	0.0	1.6442546084867933	0.0
9	0.0	0.0	0.0	1.8287739083455108	0.0
10	0.0	0.0	0.0	2.1587976222029885	0.0
11	0.0	0.0	0.0	2.532840135136931	0.0
12	0.0	0.0	0.0	2.8337004172794504	0.0
13	7.81861440079312E-5	0.0	0.0	2.9552016850677756	0.0
14	7.81861440079312E-5	0.0	0.0	3.007039098545034	0.0
15	7.81861440079312E-5	0.0	0.0	3.0802995154804655	0.0
16	7.81861440079312E-5	0.0	0.0	3.250354378697716	0.0
17	7.81861440079312E-5	0.0	0.0	3.443865085117346	0.0
18	7.81861440079312E-5	0.0	0.0	3.7511366310685155	0.0
19	7.81861440079312E-5	0.0	0.0	3.865132029032079	0.0
20	7.81861440079312E-5	0.0	0.0	3.9986739629976253	0.0
21	7.81861440079312E-5	0.0	0.0	4.1271337976026565	0.0
22	7.81861440079312E-5	0.0	0.0	4.249729671407093	0.0
23	7.81861440079312E-5	0.0	0.0	4.403678188958709	0.0
24	7.81861440079312E-5	0.0	0.0	4.513529721289853	0.0
25	7.81861440079312E-5	0.0	0.0	4.591168562289728	0.0
26	7.81861440079312E-5	0.0	0.0	4.67537503938627	0.0
27	7.81861440079312E-5	0.0	0.0	4.769823901347851	0.0
28	7.81861440079312E-5	0.0	0.0	4.860207083821019	0.0
29	7.81861440079312E-5	0.0	0.0	4.955594179510696	0.0
30	7.81861440079312E-5	0.0	0.0	5.089917974916322	0.0
31	7.81861440079312E-5	0.0	0.0	5.199534948815441	0.0
32	7.81861440079312E-5	0.0	0.0	5.29328013548095	0.0
33	7.81861440079312E-5	0.0	0.0	5.387728997442531	0.0
34	7.81861440079312E-5	0.0	0.0	5.485774422028477	0.0
35	7.81861440079312E-5	0.0	0.0	5.616345282521722	0.0
36	7.81861440079312E-5	0.0	0.0	5.724711278116715	0.0
37	7.81861440079312E-5	0.0	0.0	5.838315745360239	0.0
38	7.81861440079312E-5	0.0	0.0	5.9430851783308665	0.0
39	7.81861440079312E-5	0.0	0.0	6.046369074565344	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTAG	55	1.8189894E-12	45.000004	1
ACACGTG	30	2.1656942E-6	45.000004	42
GTAGCAC	30	2.1656942E-6	45.000004	24
ACAATCG	30	2.1656942E-6	45.000004	45
TAGTCCG	60	0.0	45.000004	1
AGTAAGA	30	2.1656942E-6	45.000004	16
TGATCCA	20	7.033585E-4	45.0	31
TGTTTCG	20	7.033585E-4	45.0	9
CGGCGTA	20	7.033585E-4	45.0	14
GCCTTAC	20	7.033585E-4	45.0	23
TCCGCAA	105	0.0	45.0	32
ACGCATT	20	7.033585E-4	45.0	15
TAGCGTC	20	7.033585E-4	45.0	32
CGACAGG	35	1.2120654E-7	45.0	2
TCTAGCG	80	0.0	45.0	1
ATGTACC	20	7.033585E-4	45.0	40
TAGCACG	90	0.0	45.0	1
CTAACGG	80	0.0	45.0	2
TACGCCT	45	3.8562575E-10	45.0	34
CATAGGT	20	7.033585E-4	45.0	31
>>END_MODULE