FastQCFastQC Report
Thu 26 May 2016
SRR1545168_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545168_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1009740
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58830.582625230257294No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC12660.12537881038683227No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCACG253.890467E-545.0000041
TGTCGAT253.890467E-545.00000430
TACCCGC207.0328783E-445.028
CGTATTA207.0328783E-445.027
CCAATCG453.8380676E-1045.024
ACCTCGT207.0328783E-445.011
TATTACG351.211738E-745.01
ACCGTTA207.0328783E-445.034
ACCCGTA207.0328783E-445.033
AAGGCGT207.0328783E-445.028
CGATCAT207.0328783E-445.010
CCCGCTA207.0328783E-445.030
TAGTACG700.045.01
AGTCGAC207.0328783E-445.028
ACGGTAC207.0328783E-445.037
GTATTCG302.1652395E-644.99999633
ACGGTCT3350.043.65671530
CGTTTTT42700.042.8922731
TCACGAC3600.042.525
CTCACGA3650.041.9178124