##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545168_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1009740
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.103856438291047	31.0	31.0	33.0	30.0	34.0
2	31.377366450769504	31.0	31.0	34.0	30.0	34.0
3	31.479822528571713	31.0	31.0	34.0	30.0	34.0
4	35.25987580961436	37.0	35.0	37.0	33.0	37.0
5	35.120642937785966	37.0	35.0	37.0	32.0	37.0
6	35.169966526036404	37.0	35.0	37.0	32.0	37.0
7	35.780140432190464	37.0	35.0	37.0	35.0	37.0
8	35.795031394220295	37.0	35.0	37.0	35.0	37.0
9	37.512102125299585	39.0	37.0	39.0	35.0	39.0
10	36.66063937251174	39.0	35.0	39.0	32.0	39.0
11	36.32127874502347	38.0	35.0	39.0	32.0	39.0
12	35.56238932794581	37.0	35.0	39.0	30.0	39.0
13	35.25491908808208	37.0	35.0	39.0	30.0	39.0
14	36.119607027551645	38.0	35.0	40.0	30.0	41.0
15	36.41084338542595	38.0	35.0	40.0	30.0	41.0
16	36.55398419395092	38.0	35.0	40.0	31.0	41.0
17	36.49919187117476	38.0	35.0	40.0	31.0	41.0
18	36.402443203200825	38.0	35.0	40.0	31.0	41.0
19	36.31441262107077	37.0	35.0	40.0	30.0	41.0
20	36.171110384851545	37.0	34.0	40.0	30.0	41.0
21	36.0352981955751	37.0	34.0	40.0	30.0	41.0
22	35.976208726999026	37.0	34.0	40.0	30.0	41.0
23	35.94690217283657	37.0	34.0	40.0	30.0	41.0
24	35.84967219284172	37.0	34.0	40.0	30.0	41.0
25	35.742258403153286	37.0	34.0	40.0	30.0	41.0
26	35.60099233466041	37.0	34.0	40.0	29.0	41.0
27	35.51874046784321	37.0	34.0	40.0	29.0	41.0
28	35.518534474221084	37.0	34.0	40.0	29.0	41.0
29	35.50946481272407	37.0	34.0	40.0	29.0	41.0
30	35.40760690870917	36.0	34.0	40.0	29.0	41.0
31	35.22734664369045	36.0	34.0	40.0	28.0	41.0
32	35.06928021074732	36.0	34.0	40.0	27.0	41.0
33	34.84817576801949	36.0	33.0	40.0	26.0	41.0
34	34.64484025590746	36.0	33.0	40.0	25.0	41.0
35	34.500258482381604	36.0	33.0	40.0	24.0	41.0
36	34.32721987838453	36.0	33.0	40.0	24.0	41.0
37	34.25136371739259	36.0	33.0	40.0	23.0	41.0
38	34.20939350723949	36.0	33.0	40.0	23.0	41.0
39	34.150264424505316	36.0	33.0	40.0	23.0	41.0
40	33.99523936855032	36.0	33.0	40.0	23.0	41.0
41	33.96432844098481	35.0	33.0	40.0	23.0	41.0
42	33.895807831719054	35.0	33.0	40.0	23.0	41.0
43	33.83917840236101	35.0	33.0	40.0	23.0	41.0
44	33.71613979836393	35.0	33.0	40.0	23.0	41.0
45	33.64676154257532	35.0	33.0	39.0	23.0	41.0
46	33.597433002555114	35.0	32.0	39.0	23.0	41.0
47	33.47858359577713	35.0	32.0	39.0	23.0	41.0
48	33.42812407154317	35.0	32.0	39.0	23.0	41.0
49	33.392652564026385	35.0	32.0	39.0	23.0	41.0
50	33.32554023808109	35.0	32.0	39.0	23.0	41.0
51	33.17477964624557	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	1.0
13	3.0
14	11.0
15	32.0
16	116.0
17	306.0
18	720.0
19	1375.0
20	2279.0
21	3733.0
22	5415.0
23	7746.0
24	10758.0
25	14810.0
26	19062.0
27	21957.0
28	24629.0
29	27929.0
30	32547.0
31	39157.0
32	48588.0
33	61617.0
34	95881.0
35	128012.0
36	77748.0
37	93206.0
38	122571.0
39	169438.0
40	91.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.12030819815001	23.85297205221146	28.850793273515958	14.175926476122566
2	31.80818824647929	25.620654822033394	30.21896725889833	12.352189672588983
3	28.54318933586864	24.859666844930377	33.08267474795492	13.514469071246063
4	25.42674351813338	27.360211539604258	32.72733574979698	14.485709192465388
5	23.010180838631726	31.61912967694654	31.276566244775882	14.09412323964585
6	20.0943807316735	41.011943668667186	28.733337294749145	10.160338304910175
7	87.51054726959416	3.808406124348842	7.142234634658427	1.538811971398578
8	88.80474181472458	2.9693782557886186	6.812050626894052	1.4138293025927466
9	85.01327074296353	4.565036544060847	8.085744845207678	2.33594786776794
10	47.74308237764177	24.11719848673916	15.44536217244043	12.69435696317864
11	41.36213282627211	22.178184483134274	21.63883772060134	14.820844969992276
12	37.76704894329233	21.433141204666548	24.93523085150633	15.864579000534789
13	24.29367956107513	33.69461445520629	25.420207182046862	16.591498801671715
14	18.59231089191277	35.28938142492127	29.21207439538891	16.90623328777705
15	16.862657713866934	23.126250321865037	42.6436508408105	17.367441123457525
16	19.98039099174045	19.956523461485133	39.870758809198406	20.19232673757601
17	20.425456057995127	20.03060193713233	29.376473151504346	30.167468853368195
18	23.662725057935706	22.794085606195654	33.01602392695149	20.52716540891715
19	28.40721373819003	24.517202448154972	26.053439499277044	21.022144314377957
20	30.184403905955985	23.971022243349775	25.565095965297996	20.27947788539624
21	23.545071008378393	26.802048051973777	29.280606888902096	20.372274050745737
22	23.691544357953536	23.33868124467685	27.30613821379761	25.66363618357201
23	21.38768395824668	27.316140788717885	26.86018182898568	24.435993424049755
24	21.399370134886208	24.26139402222354	35.58727989383406	18.751955949056192
25	20.465367322280983	24.826093845940537	32.666528017113315	22.04201081466516
26	18.805534097886582	31.219125715530733	27.216907322677127	22.758432863905558
27	18.42612949868283	30.388614890962028	29.587022401806408	21.598233208548738
28	17.69475310475964	26.907817854101058	35.333452175807636	20.06397686533167
29	18.96508011963476	24.586923366411156	34.04985441796898	22.398142095985104
30	21.66696377285242	28.038405926278053	30.702755164695862	19.59187513617367
31	27.762196208925072	25.819220789510172	26.79610592825876	19.622477073306
32	28.734921861073147	25.63432170657793	26.52415473290154	19.10660169944738
33	27.96254481351635	26.35807237506685	25.304236734208807	20.375146077207994
34	21.141779071840276	26.75698694713491	28.646780359300415	23.454453621724404
35	21.496424822231468	26.552974032919362	29.81856715590152	22.13203398894765
36	28.86693604294175	26.2655733159031	25.40426248341157	19.46322815774358
37	21.70202230277101	31.657951551884643	26.87919662487373	19.760829520470615
38	22.73634797076475	30.68047220076455	24.544140075663044	22.039039752807653
39	22.492819933844356	29.915621843246775	25.29512547784578	22.296432745063086
40	24.081248638263315	26.43215085071405	25.150731871570898	24.33586863945174
41	19.741715689187316	24.52056965159348	27.654742805078534	28.08297185414067
42	22.253352348129223	26.253689068473072	25.190048923485254	26.302909659912455
43	21.72420623130707	26.112167488660447	27.226216649830647	24.937409630201834
44	21.658842870441898	28.19696159407372	27.457761403925762	22.68643413155862
45	19.899181967635233	33.59310317507477	23.749381028779688	22.75833382851031
46	22.489551765801096	30.420702359023117	26.092855586586644	20.996890288589142
47	21.58813159823321	28.395824667736246	26.877314952363974	23.13872878166657
48	22.04864618614693	26.805811396993285	29.29100560540337	21.854536811456416
49	22.374175530334544	25.64531463545071	29.760532414284867	22.219977419929883
50	21.099689028858915	30.465367322280983	26.613187553231526	21.82175609562858
51	20.318200724939093	31.17376750450611	25.661061263295505	22.846970507259297
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	761.0
1	1085.5
2	1410.0
3	5701.0
4	9992.0
5	7195.0
6	4398.0
7	4655.0
8	4912.0
9	5159.5
10	5407.0
11	5524.0
12	5641.0
13	5609.0
14	5577.0
15	5252.5
16	4928.0
17	4766.0
18	4604.0
19	4354.5
20	4105.0
21	4000.5
22	3896.0
23	4081.5
24	4267.0
25	5310.5
26	6794.5
27	7235.0
28	8778.0
29	10321.0
30	11975.0
31	13629.0
32	16157.5
33	18686.0
34	21935.0
35	25184.0
36	28009.0
37	30834.0
38	35975.0
39	41116.0
40	52780.0
41	64444.0
42	71911.0
43	79378.0
44	80202.0
45	81026.0
46	79697.5
47	78369.0
48	75509.5
49	72650.0
50	68654.5
51	64659.0
52	62507.5
53	60356.0
54	56901.5
55	53447.0
56	53756.5
57	54066.0
58	50715.5
59	47365.0
60	42333.5
61	37302.0
62	34649.5
63	31997.0
64	27912.5
65	23828.0
66	19806.5
67	15785.0
68	13871.5
69	11958.0
70	9585.5
71	7213.0
72	6453.0
73	5693.0
74	4313.5
75	2419.5
76	1905.0
77	1607.0
78	1309.0
79	880.0
80	451.0
81	331.0
82	211.0
83	131.0
84	51.0
85	52.0
86	53.0
87	34.0
88	15.0
89	13.5
90	12.0
91	8.0
92	4.0
93	3.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1009740.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.989063619122906
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.01535497555415	16.1648018523124
2	6.875565120188659	2.886233474501244
3	2.6744820522906085	1.6840462183118992
4	1.5875179571827962	1.3328206159923897
5	1.100287193542387	1.1546998952283676
6	0.8672211503180939	1.092129593752525
7	0.6856165617323084	1.0073314742766608
8	0.567825248825048	0.9534496217706581
9	0.4797058635046871	0.906171919880955
>10	5.9336159821276535	28.510643204718573
>50	1.477566347127229	22.102891528709474
>100	0.7328804636384129	21.29769034126975
>500	0.0014166503807443546	0.19852510809966126
>1k	4.722167935814515E-4	0.12547817616284132
>5k	4.722167935814515E-4	0.5830869750126346
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5883	0.582625230257294	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1266	0.12537881038683227	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.023669459464812723	0.0
2	0.0	0.0	0.0	0.09457880246400063	0.0
3	0.0	0.0	0.0	0.1349852437261077	0.0
4	0.0	0.0	0.0	0.1843048705607384	0.0
5	0.0	0.0	0.0	0.3043357696040565	0.0
6	0.0	0.0	0.0	0.4198110404658625	0.0
7	0.0	0.0	0.0	0.4902252064887991	0.0
8	0.0	0.0	0.0	0.632836175649177	0.0
9	0.0	0.0	0.0	0.6975062887475983	0.0
10	0.0	0.0	0.0	0.81506130290966	0.0
11	0.0	0.0	0.0	0.9786677758630935	0.0
12	0.0	0.0	0.0	1.098896745696912	0.0
13	0.0	0.0	0.0	1.1491076910887952	0.0
14	0.0	0.0	0.0	1.1726781151583576	0.0
15	0.0	0.0	0.0	1.2092221760057045	0.0
16	0.0	0.0	0.0	1.2888466337869156	0.0
17	0.0	0.0	0.0	1.3729276843543883	0.0
18	0.0	0.0	0.0	1.4973161407887179	0.0
19	0.0	0.0	0.0	1.5537663160813675	0.0
20	0.0	0.0	0.0	1.6187335353655397	0.0
21	0.0	0.0	0.0	1.702220373561511	0.0
22	0.0	0.0	0.0	1.7922435478439995	0.0
23	0.0	0.0	0.0	1.8910808723037613	0.0
24	0.0	0.0	0.0	1.966446808089211	0.0
25	0.0	0.0	0.0	2.029037177887377	0.0
26	0.0	0.0	0.0	2.0975696714005587	0.0
27	0.0	0.0	0.0	2.1741240319290114	0.0
28	0.0	0.0	0.0	2.2475092598094557	0.0
29	0.0	0.0	0.0	2.325747222057163	0.0
30	0.0	0.0	0.0	2.4265652544219303	0.0
31	0.0	0.0	0.0	2.510943411175154	0.0
32	0.0	0.0	0.0	2.5929447184423715	0.0
33	0.0	0.0	0.0	2.676233485847842	0.0
34	0.0	0.0	0.0	2.7707132529165923	0.0
35	0.0	0.0	0.0	2.884108780478143	0.0
36	0.0	0.0	0.0	2.976211698060887	0.0
37	0.0	0.0	0.0	3.0729692792203935	0.0
38	0.0	0.0	0.0	3.1816111078099314	0.0
39	0.0	0.0	0.0	3.3537346247548876	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCACG	25	3.890467E-5	45.000004	1
TGTCGAT	25	3.890467E-5	45.000004	30
TACCCGC	20	7.0328783E-4	45.0	28
CGTATTA	20	7.0328783E-4	45.0	27
CCAATCG	45	3.8380676E-10	45.0	24
ACCTCGT	20	7.0328783E-4	45.0	11
TATTACG	35	1.211738E-7	45.0	1
ACCGTTA	20	7.0328783E-4	45.0	34
ACCCGTA	20	7.0328783E-4	45.0	33
AAGGCGT	20	7.0328783E-4	45.0	28
CGATCAT	20	7.0328783E-4	45.0	10
CCCGCTA	20	7.0328783E-4	45.0	30
TAGTACG	70	0.0	45.0	1
AGTCGAC	20	7.0328783E-4	45.0	28
ACGGTAC	20	7.0328783E-4	45.0	37
GTATTCG	30	2.1652395E-6	44.999996	33
ACGGTCT	335	0.0	43.656715	30
CGTTTTT	4270	0.0	42.892273	1
TCACGAC	360	0.0	42.5	25
CTCACGA	365	0.0	41.91781	24
>>END_MODULE