FastQCFastQC Report
Thu 26 May 2016
SRR1545167_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545167_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1329300
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79710.5996389076957798No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTACG650.045.0000041
CGTAAAT302.1657597E-645.00000416
GTCGTTA207.033687E-445.039
CGATCGA253.891137E-545.041
CGTTTTT53900.043.0797771
CGGTCTA2700.042.531
ACGTAAG700.041.7857131
ACTACGG603.6379788E-1241.2500042
TAACGCG556.184564E-1140.909091
CACGACG2950.039.6610226
CAGGGTA2500.039.6000025
TCTACCG403.458772E-739.3751
TACGACT403.458772E-739.37517
CTCACGA3050.039.09836224
ATTTACG750.039.01
CGTATGG750.039.02
ACGACGG3000.039.027
CATATGC6650.038.90977533
ACGGGAC3300.038.863645
GGCGATT7650.038.823538
Produced by FastQC (version 0.11.5)