##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545167_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1329300
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.018102760851576	31.0	31.0	33.0	30.0	34.0
2	31.30190626645603	31.0	31.0	34.0	28.0	34.0
3	31.416736628300608	31.0	31.0	34.0	30.0	34.0
4	35.207904912359886	37.0	35.0	37.0	32.0	37.0
5	35.0626781012563	37.0	35.0	37.0	32.0	37.0
6	35.10395396073121	37.0	35.0	37.0	32.0	37.0
7	35.72904235311818	37.0	35.0	37.0	35.0	37.0
8	35.72760099300384	37.0	35.0	37.0	35.0	37.0
9	37.42117054088618	39.0	37.0	39.0	35.0	39.0
10	36.560469419995485	39.0	35.0	39.0	32.0	39.0
11	36.25286015195968	38.0	35.0	39.0	31.0	39.0
12	35.66711953659821	37.0	35.0	39.0	30.0	39.0
13	35.42847889866847	37.0	35.0	39.0	30.0	39.0
14	36.36472730008275	38.0	35.0	40.0	30.0	41.0
15	36.59592717971865	38.0	35.0	40.0	31.0	41.0
16	36.68066651621154	38.0	35.0	40.0	31.0	41.0
17	36.60472955690965	38.0	35.0	40.0	31.0	41.0
18	36.508451816745655	38.0	35.0	40.0	31.0	41.0
19	36.438961859625365	38.0	35.0	40.0	30.0	41.0
20	36.30534115699992	38.0	35.0	40.0	30.0	41.0
21	36.17789513277665	38.0	34.0	40.0	30.0	41.0
22	36.11879410215903	38.0	34.0	40.0	30.0	41.0
23	36.051452644248855	38.0	34.0	40.0	30.0	41.0
24	35.96749642669074	38.0	34.0	40.0	30.0	41.0
25	35.842344090874896	37.0	34.0	40.0	30.0	41.0
26	35.69904686677199	37.0	34.0	40.0	29.0	41.0
27	35.60601143458963	37.0	34.0	40.0	29.0	41.0
28	35.59302565259911	37.0	34.0	40.0	29.0	41.0
29	35.53223651545927	37.0	34.0	40.0	29.0	41.0
30	35.45234709997743	37.0	34.0	40.0	28.0	41.0
31	35.309549386895355	37.0	34.0	40.0	27.0	41.0
32	35.16499435793275	37.0	34.0	40.0	27.0	41.0
33	34.95987737907169	37.0	33.0	40.0	26.0	41.0
34	34.752293688407434	37.0	33.0	40.0	25.0	41.0
35	34.61443391258557	37.0	33.0	40.0	24.0	41.0
36	34.45779207101482	37.0	33.0	40.0	24.0	41.0
37	34.39671330775596	37.0	33.0	40.0	24.0	41.0
38	34.329652448657185	37.0	33.0	40.0	24.0	41.0
39	34.26388174227037	36.0	33.0	40.0	23.0	41.0
40	34.11063792973746	36.0	33.0	40.0	23.0	41.0
41	34.02210261039645	36.0	33.0	40.0	23.0	41.0
42	33.957154141277364	36.0	33.0	40.0	23.0	41.0
43	33.89758143383736	36.0	33.0	40.0	23.0	41.0
44	33.77624464003611	35.0	33.0	40.0	23.0	41.0
45	33.70282178590235	35.0	32.0	40.0	23.0	41.0
46	33.62438426239374	35.0	32.0	40.0	23.0	41.0
47	33.50894305273452	35.0	32.0	39.0	23.0	41.0
48	33.4351937109757	35.0	32.0	39.0	23.0	41.0
49	33.37524185661626	35.0	32.0	39.0	23.0	41.0
50	33.33575866997668	35.0	32.0	39.0	23.0	41.0
51	33.201231475212516	35.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	2.0
13	8.0
14	18.0
15	55.0
16	167.0
17	489.0
18	979.0
19	1945.0
20	3207.0
21	5082.0
22	7411.0
23	10349.0
24	14140.0
25	19509.0
26	24766.0
27	28919.0
28	32886.0
29	37170.0
30	43430.0
31	52128.0
32	62946.0
33	78055.0
34	115569.0
35	147224.0
36	109771.0
37	133231.0
38	172247.0
39	227483.0
40	112.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.332505830136164	23.024524185661626	27.584969532836833	14.058000451365379
2	32.45708267509215	24.564056270217407	29.20875648837734	13.770104566313096
3	29.504626495147825	25.462423832092078	31.663281426314597	13.3696682464455
4	26.53103136989393	27.745655608214847	30.919130369367338	14.804182652523885
5	23.52689385390807	31.906642593846385	30.22921838561649	14.337245166629053
6	20.31798691040397	41.512149251485745	27.421424810050404	10.748439028059881
7	87.24178138870083	3.7910930564959	7.175957270744	1.791168284059279
8	88.42172572030393	3.018581208154668	6.813209960129392	1.7464831114120214
9	84.19867599488452	4.688031294666366	8.248025276461297	2.865267433987813
10	43.65651094561047	28.610396449259007	15.023997592717972	12.709095012412547
11	37.209057398630854	23.135334386519222	23.37034529451591	16.28526292033401
12	33.614609192808246	21.981268336718575	27.04423380726698	17.3598886632062
13	24.838862559241708	29.562326036259684	27.6592943654555	17.939517039043103
14	19.856691491762582	32.019559166478594	29.759798390130143	18.363950951628677
15	19.062438877604755	23.114571579026556	38.10012788685774	19.722861656510947
16	21.643722259836004	20.687956067102988	36.453246069359814	21.215075603701198
17	21.979538102760852	20.81140449860829	29.082599864590385	28.126457534040473
18	24.15647333182878	23.31610622131949	31.642969984202214	20.884450462649514
19	28.40412247047318	24.97878582712706	25.82705183179117	20.790039870608588
20	29.62694651320244	24.417437749191304	25.76980365605958	20.18581208154668
21	24.929060407733395	25.862258331452644	28.79823967501693	20.410441585797038
22	24.75505905363725	23.93605657112766	27.181298427743926	24.127585947491163
23	22.818626344692696	26.376062589332733	26.521703152034902	24.28360791393967
24	22.305047769502746	24.180696607236893	33.54133754607688	19.97291807718348
25	22.028661701647483	25.259384638531557	30.742721733243062	21.969231926577898
26	20.7581433837358	29.41969457609268	26.822312495298277	22.999849544873243
27	20.351387948544346	28.195365982095836	28.720755284736327	22.732490784623486
28	20.214473783194162	26.68584969532837	32.096140825998646	21.003535695478824
29	20.75250131648236	24.338975400586772	32.4174377491913	22.491085533739565
30	22.64515158354021	26.57744677649891	30.129918001955918	20.647483638004964
31	27.54682915820357	26.051455653351386	25.786428947566385	20.615286240878657
32	28.342812006319118	24.619198074174378	25.868953584593395	21.169036334913113
33	27.407658165952004	25.65741367637102	25.214398555630783	21.72052960204619
34	21.783720755284737	27.276386067855263	27.665312570525842	23.274580606334162
35	22.372677348980666	25.452869931542914	29.025652599112316	23.1488001203641
36	27.37425712781163	26.20123373203942	25.733167832693898	20.69134130745505
37	22.50214398555631	30.58489430527345	25.847664184157075	21.065297525013165
38	23.35402091326262	29.23124952982773	25.340404724290984	22.07432483261867
39	22.560294892048447	28.398856541036636	25.65575866997668	23.38508989693824
40	25.082675092153767	25.56759196569623	26.15955766192733	23.190175280222675
41	20.805386293537953	24.939742721733243	27.943729782592342	26.31114120213646
42	22.483186639584744	26.22365154592643	25.045663130971185	26.24749868351764
43	22.335289249981194	26.00210637177462	26.56548559392161	25.097118784322575
44	22.75618746708794	26.485970059429775	27.574813811780636	23.183028661701645
45	20.583991574512904	30.57556608741443	24.835025953509366	24.005416384563304
46	23.343413826826147	28.433686902881217	26.58805386293538	21.634845407357258
47	21.78138870081998	27.66418415707515	27.529752501316484	23.024674640788383
48	22.699917249680283	26.2146994658843	28.770706386820134	22.31467689761529
49	22.758068156172424	25.49063416835929	29.35710524336117	22.394192432107126
50	21.146618521026102	28.867072895508915	27.573083577822917	22.41322500564207
51	21.5068833220492	29.08335214022418	26.149326713307758	23.260437824418865
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	720.0
1	1239.5
2	1759.0
3	7590.5
4	13422.0
5	9563.5
6	5705.0
7	5793.5
8	5882.0
9	6216.0
10	6550.0
11	6541.5
12	6533.0
13	6485.5
14	6438.0
15	6170.5
16	5903.0
17	5424.0
18	4945.0
19	4997.5
20	5050.0
21	5253.0
22	5456.0
23	5703.5
24	5951.0
25	6177.0
26	8079.5
27	9756.0
28	11674.0
29	13592.0
30	16524.5
31	19457.0
32	23199.0
33	26941.0
34	31050.0
35	35159.0
36	37676.0
37	40193.0
38	45633.0
39	51073.0
40	59951.5
41	68830.0
42	78710.5
43	88591.0
44	93299.0
45	98007.0
46	95684.0
47	93361.0
48	94823.0
49	96285.0
50	92928.0
51	89571.0
52	85129.0
53	80687.0
54	76930.5
55	73174.0
56	72990.5
57	72807.0
58	69304.5
59	65802.0
60	61796.5
61	57791.0
62	52079.0
63	46367.0
64	42409.5
65	38452.0
66	32297.5
67	26143.0
68	22811.0
69	19479.0
70	16487.5
71	13496.0
72	11772.5
73	10049.0
74	8421.5
75	4924.0
76	3054.0
77	2370.5
78	1687.0
79	1305.5
80	924.0
81	776.0
82	628.0
83	463.0
84	298.0
85	198.5
86	99.0
87	59.0
88	19.0
89	14.5
90	10.0
91	7.0
92	4.0
93	2.5
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1329300.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.64403204344873
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.04568139445091	16.459351648673884
2	6.940131619722655	3.0042486232605774
3	3.002969062104819	1.9498907581704565
4	1.8237140121737665	1.5789009807030179
5	1.2150731344544385	1.3149540928632777
6	0.9131589732501888	1.1858665246673896
7	0.7264062710228514	1.100565242460649
8	0.6210276013332527	1.075323304249842
9	0.5426552789911766	1.0570723422328474
>10	6.1197908808843255	29.972715288545633
>50	1.4226952983836878	21.81633510575727
>100	0.6235612490706578	18.48777816556357
>500	0.002786865917633616	0.3959943349328051
>1k	0.0	0.0
>5k	3.48358239704202E-4	0.6010035879187718
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7971	0.5996389076957798	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02143985556307831	0.0
2	0.0	0.0	0.0	0.08786579402693147	0.0
3	0.0	0.0	0.0	0.12585571353343866	0.0
4	0.0	0.0	0.0	0.17467840216655384	0.0
5	0.0	0.0	0.0	0.3032423079816445	0.0
6	0.0	0.0	0.0	0.4465508162190627	0.0
7	0.0	0.0	0.0	0.5357707063868201	0.0
8	0.0	0.0	0.0	0.7701797938764764	0.0
9	0.0	0.0	0.0	0.8725645076355977	0.0
10	0.0	0.0	0.0	1.0550665763935907	0.0
11	7.522756337922214E-5	0.0	0.0	1.2032648762506581	0.0
12	1.5045512675844429E-4	0.0	0.0	1.3272399006996163	0.0
13	2.2568269013766644E-4	0.0	0.0	1.3839614834875498	0.0
14	2.2568269013766644E-4	0.0	0.0	1.4075829383886256	0.0
15	2.2568269013766644E-4	0.0	0.0	1.4421123899796886	0.0
16	2.2568269013766644E-4	0.0	0.0	1.517114270668773	0.0
17	2.2568269013766644E-4	0.0	0.0	1.6032498307379823	0.0
18	2.2568269013766644E-4	0.0	0.0	1.730459640412247	0.0
19	3.761378168961107E-4	0.0	0.0	1.7848491687354247	0.0
20	3.761378168961107E-4	0.0	0.0	1.8484916873542465	0.0
21	3.761378168961107E-4	0.0	0.0	1.9269540359587753	0.0
22	3.761378168961107E-4	0.0	0.0	2.012939140901226	0.0
23	3.761378168961107E-4	0.0	0.0	2.10832769126608	0.0
24	3.761378168961107E-4	0.0	0.0	2.186112991800196	0.0
25	3.761378168961107E-4	0.0	0.0	2.248852779658467	0.0
26	3.761378168961107E-4	0.0	0.0	2.3136989392913563	0.0
27	3.761378168961107E-4	0.0	0.0	2.386067855262168	0.0
28	3.761378168961107E-4	0.0	0.0	2.4555028962611902	0.0
29	3.761378168961107E-4	0.0	0.0	2.536071616640337	0.0
30	3.761378168961107E-4	0.0	0.0	2.6480102309486195	0.0
31	3.761378168961107E-4	0.0	0.0	2.7370796659896186	0.0
32	3.761378168961107E-4	0.0	0.0	2.8220115850447605	0.0
33	3.761378168961107E-4	0.0	0.0	2.9104039720153465	0.0
34	3.761378168961107E-4	0.0	0.0	2.999247724366208	0.0
35	3.761378168961107E-4	0.0	0.0	3.111336793801249	0.0
36	4.513653802753329E-4	0.0	0.0	3.2009328217859023	0.0
37	4.513653802753329E-4	0.0	0.0	3.2978259234183405	0.0
38	4.513653802753329E-4	0.0	0.0	3.4098397652900023	0.0
39	4.513653802753329E-4	0.0	0.0	3.5763935906116	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGTACG	65	0.0	45.000004	1
CGTAAAT	30	2.1657597E-6	45.000004	16
GTCGTTA	20	7.033687E-4	45.0	39
CGATCGA	25	3.891137E-5	45.0	41
CGTTTTT	5390	0.0	43.079777	1
CGGTCTA	270	0.0	42.5	31
ACGTAAG	70	0.0	41.785713	1
ACTACGG	60	3.6379788E-12	41.250004	2
TAACGCG	55	6.184564E-11	40.90909	1
CACGACG	295	0.0	39.66102	26
CAGGGTA	250	0.0	39.600002	5
TCTACCG	40	3.458772E-7	39.375	1
TACGACT	40	3.458772E-7	39.375	17
CTCACGA	305	0.0	39.098362	24
ATTTACG	75	0.0	39.0	1
CGTATGG	75	0.0	39.0	2
ACGACGG	300	0.0	39.0	27
CATATGC	665	0.0	38.909775	33
ACGGGAC	330	0.0	38.86364	5
GGCGATT	765	0.0	38.82353	8
>>END_MODULE