##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545166_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	956050
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.121451806913864	31.0	31.0	33.0	30.0	34.0
2	31.400573191778673	31.0	31.0	34.0	30.0	34.0
3	31.512945975628888	31.0	31.0	34.0	30.0	34.0
4	35.27810260969615	37.0	35.0	37.0	33.0	37.0
5	35.13957219810679	37.0	35.0	37.0	32.0	37.0
6	35.19470634381047	37.0	35.0	37.0	32.0	37.0
7	35.78191412583024	37.0	35.0	37.0	35.0	37.0
8	35.79975315098583	37.0	35.0	37.0	35.0	37.0
9	37.56167982846085	39.0	37.0	39.0	35.0	39.0
10	36.76612729459756	39.0	37.0	39.0	32.0	39.0
11	36.33418649652215	38.0	35.0	39.0	32.0	39.0
12	35.019703990377074	35.0	34.0	39.0	30.0	39.0
13	34.4158726008054	35.0	33.0	39.0	26.0	39.0
14	35.23472203336645	36.0	33.0	40.0	26.0	41.0
15	35.75048480727995	36.0	33.0	40.0	30.0	41.0
16	36.032677161236336	36.0	34.0	40.0	31.0	41.0
17	36.00196537837979	36.0	34.0	40.0	31.0	41.0
18	35.94317242822028	36.0	35.0	40.0	31.0	41.0
19	35.84559803357565	36.0	34.0	40.0	30.0	41.0
20	35.68115056743894	36.0	34.0	40.0	30.0	41.0
21	35.48839286648188	35.0	34.0	40.0	30.0	41.0
22	35.37716542021861	35.0	34.0	40.0	30.0	41.0
23	35.39798127712986	35.0	34.0	40.0	30.0	41.0
24	35.29439882851315	35.0	34.0	40.0	30.0	41.0
25	35.20909366664923	35.0	34.0	40.0	29.0	41.0
26	35.05243763401496	35.0	34.0	40.0	29.0	41.0
27	34.995862141101405	35.0	34.0	40.0	29.0	41.0
28	35.02302390042362	35.0	34.0	40.0	29.0	41.0
29	35.0936938444642	36.0	34.0	40.0	29.0	41.0
30	35.056302494639404	36.0	34.0	40.0	29.0	41.0
31	34.81812771298572	35.0	34.0	40.0	29.0	41.0
32	34.60570053867475	35.0	34.0	40.0	27.0	41.0
33	34.36431567386643	35.0	33.0	40.0	26.0	41.0
34	34.24834893572512	35.0	33.0	40.0	25.0	41.0
35	34.100134930181476	35.0	33.0	39.0	25.0	41.0
36	33.847635583912975	35.0	33.0	39.0	23.0	41.0
37	33.79215208409602	35.0	33.0	39.0	23.0	41.0
38	33.82846190052822	35.0	33.0	39.0	24.0	41.0
39	33.82046231891638	35.0	33.0	39.0	23.0	41.0
40	33.61457768945139	35.0	33.0	39.0	23.0	41.0
41	33.66216097484441	35.0	33.0	39.0	23.0	41.0
42	33.6116290988965	35.0	33.0	39.0	23.0	41.0
43	33.490823701689244	35.0	33.0	39.0	23.0	41.0
44	33.39492599759427	35.0	33.0	39.0	23.0	41.0
45	33.36116939490613	35.0	32.0	39.0	23.0	41.0
46	33.31812039119293	35.0	32.0	38.0	23.0	41.0
47	33.22512107107369	35.0	32.0	38.0	23.0	40.0
48	33.23508080121333	35.0	32.0	38.0	23.0	40.0
49	33.26355839129753	35.0	32.0	38.0	24.0	40.0
50	33.150855080801215	35.0	32.0	38.0	23.0	40.0
51	32.95727001725851	35.0	32.0	38.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	6.0
15	20.0
16	102.0
17	260.0
18	646.0
19	1292.0
20	2190.0
21	3493.0
22	5387.0
23	7722.0
24	10629.0
25	14761.0
26	18577.0
27	21611.0
28	23747.0
29	26953.0
30	32271.0
31	39423.0
32	49176.0
33	63905.0
34	110997.0
35	165856.0
36	59309.0
37	69707.0
38	92437.0
39	135494.0
40	78.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.51655248156477	24.317138224988234	28.46273730453428	13.703571988912714
2	29.746456775273263	25.589979603577216	31.207259034569322	13.4563045865802
3	27.710893781705977	24.458971811097747	33.92688666910726	13.90324773808901
4	24.34809894879975	28.716908111500445	32.85570838345275	14.079284556247057
5	22.446210972229487	33.354845457873544	31.096909157470844	13.102034412426129
6	19.21081533392605	41.6921709115632	28.17927932639506	10.917734428115685
7	87.83839757334867	4.498927880340986	6.175618429998432	1.4870561163119083
8	90.08399142304273	2.8929449296584906	5.691334135243973	1.331729512054809
9	87.10925160817948	4.420898488572774	6.558861984205847	1.910987919041891
10	58.597249097850536	22.151351916740754	11.333193870613462	7.918205114795251
11	53.886826002824115	18.44788452486795	17.53705350138591	10.128235970922022
12	49.01961194498195	20.045918100517756	19.585691124941164	11.348778829559123
13	21.402437111029755	48.25322943360703	18.69598870351969	11.648344751843522
14	13.93975210501543	49.24125307253805	23.404529051827833	13.414465770618692
15	11.79498980178861	21.364782176664402	53.2509805972491	13.589247424297893
16	12.267559228073846	15.988912713770201	52.1552220072172	19.58830605093876
17	14.311803775953141	17.10234820354584	27.212384289524604	41.37346373097641
18	20.117985461011454	22.51984728832174	36.416714606976626	20.94545264369018
19	30.301867057162283	22.97233408294545	24.582396318184195	22.14340254170807
20	33.996966685842786	21.626797761623344	23.11814235657131	21.258093195962555
21	20.98028345797814	30.742115998117253	27.21698655928037	21.060613984624236
22	22.564719418440458	25.120025103289578	23.909941948642853	28.40531352962711
23	17.956801422519742	32.606558234401966	22.70540243711103	26.73123790596726
24	18.18670571622823	22.415459442497777	42.967836410229594	16.429998431044403
25	15.101302233146802	24.81188222373307	38.691072642644215	21.395742900475916
26	14.721196590136499	37.29919983264474	27.295329742168295	20.68427383505047
27	15.577009570629151	37.97081742586685	27.50881230061189	18.943360702892107
28	12.999843104440146	29.102139009466033	41.14795251294389	16.75006537314994
29	14.02269755765912	22.900998901731082	40.23837665394069	22.83792688666911
30	17.02431881177763	32.769311228492235	32.08576957272109	18.12060038700905
31	30.033261858689396	27.43182887924272	23.96998064954762	18.564928612520266
32	32.438993776476124	27.20684064640971	24.367867789341563	15.986297787772605
33	30.38397573348674	29.22849223366979	22.64253961612886	17.74499241671461
34	18.935097536739708	29.24073008733853	26.245907640813765	25.578264735107997
35	18.58197793002458	26.526646095915485	32.23701689242194	22.65435908163799
36	32.85738193609121	24.554050520370275	25.351079964437005	17.23748757910151
37	19.26248627163851	34.458030437738614	28.06464097066053	18.214842319962347
38	19.7780450813242	34.46995450028764	23.48820668375085	22.26379373463731
39	19.77783588724439	33.87040426755923	26.777156006485015	19.574603838711365
40	25.24292662517651	25.69813294283772	24.458448825898227	24.600491606087548
41	16.460645363736205	24.521416243920296	27.384760211286018	31.633178181057474
42	22.416087024737198	25.309659536635117	22.622666178547146	29.651587260080543
43	22.371737879818003	27.00768788243293	23.91088332200199	26.709690915747085
44	18.878092149992153	31.21468542440249	28.596726112651012	21.310496312954346
45	16.050101982113908	41.7448878196747	20.01495737670624	22.19005282150515
46	22.072067360493698	35.473981486323936	24.328330108257937	18.12562104492443
47	21.343339783484126	29.232362324146226	24.305737147638723	25.11856074473092
48	22.546310339417396	24.17729198263689	30.196433240939278	23.079964437006435
49	20.90288164844935	22.784896187437894	33.88829036138277	22.42393180272998
50	18.369959730139637	35.2040165263323	25.01417289890696	21.411850844621096
51	17.21248888656451	36.89932534909262	22.50541289681502	23.382772867527848
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	719.0
1	899.5
2	1080.0
3	3516.5
4	5953.0
5	4540.0
6	3127.0
7	3523.5
8	3920.0
9	4429.0
10	4938.0
11	5237.5
12	5537.0
13	5385.0
14	5233.0
15	4967.5
16	4702.0
17	4489.5
18	4277.0
19	3855.5
20	3434.0
21	3650.0
22	3866.0
23	3822.5
24	3779.0
25	3503.5
26	4206.0
27	5184.0
28	6959.0
29	8734.0
30	10701.0
31	12668.0
32	13957.5
33	15247.0
34	18974.5
35	22702.0
36	25428.0
37	28154.0
38	32961.0
39	37768.0
40	57688.0
41	77608.0
42	93527.5
43	109447.0
44	109155.5
45	108864.0
46	100253.0
47	91642.0
48	82658.0
49	73674.0
50	71483.0
51	69292.0
52	63284.5
53	57277.0
54	48662.0
55	40047.0
56	40128.5
57	40210.0
58	34468.5
59	28727.0
60	25262.0
61	21797.0
62	19493.0
63	17189.0
64	13896.0
65	10603.0
66	9184.0
67	7765.0
68	6752.5
69	5740.0
70	5119.5
71	4499.0
72	3929.0
73	3359.0
74	2570.5
75	1396.0
76	1010.0
77	974.5
78	939.0
79	597.0
80	255.0
81	151.0
82	47.0
83	30.5
84	14.0
85	9.5
86	5.0
87	5.0
88	5.0
89	4.0
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	956050.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.582911282793347
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.16115687470263	14.270494209367692
2	7.339081880699934	2.5808485121100615
3	2.43784033612216	1.2859299105495314
4	1.1774838925920037	0.8281437928145348
5	0.7749746480754568	0.6813155241762375
6	0.49023116594704935	0.5171814659344386
7	0.38482273652343413	0.47364128251253107
8	0.3185925896847112	0.4481428191825328
9	0.2751913709751674	0.4354798915481976
>10	3.064881276273903	12.567927467156762
>50	0.98379081640414	12.614577048196548
>100	1.5818163854061718	51.64158984314725
>500	0.008943552876401273	0.9620781168922996
>1k	0.001192473716853503	0.6926501164114006
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3552	0.37152868573819364	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3055	0.31954395690601956	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.033680246849014175	0.0
2	0.0	0.0	0.0	0.1039694576643481	0.0
3	0.0	0.0	0.0	0.14120600387009047	0.0
4	0.0	0.0	0.0	0.20605616861042833	0.0
5	0.0	0.0	0.0	0.3326185868939909	0.0
6	0.0	0.0	0.0	0.47894984571936616	0.0
7	0.0	0.0	0.0	0.5772710632289106	0.0
8	0.0	0.0	0.0	0.8402280215469902	0.0
9	0.0	0.0	0.0	0.9668950368704565	0.0
10	0.0	0.0	0.0	1.1716960410020396	0.0
11	0.0	0.0	0.0	1.3558914282725798	0.0
12	0.0	0.0	0.0	1.5350661576277391	0.0
13	1.0459703990377072E-4	0.0	0.0	1.6051461743632656	0.0
14	1.0459703990377072E-4	0.0	0.0	1.6324460017781497	0.0
15	1.0459703990377072E-4	0.0	0.0	1.676167564457926	0.0
16	1.0459703990377072E-4	0.0	0.0	1.7678991684535328	0.0
17	1.0459703990377072E-4	0.0	0.0	1.876366298833743	0.0
18	1.0459703990377072E-4	0.0	0.0	2.058469745306208	0.0
19	1.0459703990377072E-4	0.0	0.0	2.12593483604414	0.0
20	1.0459703990377072E-4	0.0	0.0	2.193818314941687	0.0
21	1.0459703990377072E-4	0.0	0.0	2.2802154699022017	0.0
22	1.0459703990377072E-4	0.0	0.0	2.3675539982218505	0.0
23	1.0459703990377072E-4	0.0	0.0	2.470895873646776	0.0
24	1.0459703990377072E-4	0.0	0.0	2.5476701009361435	0.0
25	1.0459703990377072E-4	0.0	0.0	2.6069766225615814	0.0
26	1.0459703990377072E-4	0.0	0.0	2.66879347314471	0.0
27	1.0459703990377072E-4	0.0	0.0	2.738664295800429	0.0
28	1.0459703990377072E-4	0.0	0.0	2.8039328487003816	0.0
29	1.0459703990377072E-4	0.0	0.0	2.8740128654359083	0.0
30	1.0459703990377072E-4	0.0	0.0	2.9681502013493017	0.0
31	1.0459703990377072E-4	0.0	0.0	3.0531875947910674	0.0
32	1.0459703990377072E-4	0.0	0.0	3.123895193766016	0.0
33	1.0459703990377072E-4	0.0	0.0	3.1999372417760577	0.0
34	1.0459703990377072E-4	0.0	0.0	3.278698812823597	0.0
35	1.0459703990377072E-4	0.0	0.0	3.3943831389571675	0.0
36	1.0459703990377072E-4	0.0	0.0	3.4783745619998956	0.0
37	1.0459703990377072E-4	0.0	0.0	3.5646671199205064	0.0
38	1.0459703990377072E-4	0.0	0.0	3.657444694315151	0.0
39	1.0459703990377072E-4	0.0	0.0	3.756079702944407	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGTC	30	2.1651176E-6	45.000004	45
CTTCGTA	20	7.032689E-4	45.000004	22
AACGTGT	20	7.032689E-4	45.000004	22
TAATACG	30	2.1651176E-6	45.000004	1
GTACCGG	20	7.032689E-4	45.000004	2
GCGACAA	20	7.032689E-4	45.000004	25
ATAGGCG	20	7.032689E-4	45.000004	33
CGACGTG	40	6.8121153E-9	45.000004	30
TAGACTT	20	7.032689E-4	45.000004	43
TTAGCCG	20	7.032689E-4	45.000004	2
TTTCGCG	20	7.032689E-4	45.000004	1
TTTCGAA	30	2.1651176E-6	45.000004	35
TAGGTGC	20	7.032689E-4	45.000004	27
CGTGAAT	20	7.032689E-4	45.000004	3
AGCCACG	20	7.032689E-4	45.000004	45
CACGCAT	40	6.8121153E-9	45.000004	36
TTCGTTA	40	6.8121153E-9	45.000004	15
TTCGTCG	30	2.1651176E-6	45.000004	31
TTCGTAA	30	2.1651176E-6	45.000004	13
GGATACG	40	6.8121153E-9	45.000004	8
>>END_MODULE