FastQCFastQC Report
Thu 26 May 2016
SRR1545165_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545165_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1211044
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54570.4506029508424137No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC21410.17678961292901No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTA351.2119926E-745.00000421
GCGATAC453.8562575E-1045.0000049
CGTAAGA351.2119926E-745.00000413
ACGTAAG351.2119926E-745.0000041
CACGTAC351.2119926E-745.00000422
GTGCATT406.8157533E-945.09
TTGATAC502.1827873E-1145.012
ATCTCGT253.8909297E-545.019
ACACGTC207.0334366E-445.013
GGCGTAT253.8909297E-545.08
TAGCGAA207.0334366E-445.016
GTTAGCG406.8157533E-945.01
CTAAGCG253.8909297E-545.01
ATGTCGA207.0334366E-445.025
ACTTACC207.0334366E-445.012
CCGCTAA253.8909297E-545.018
ACCACGT207.0334366E-445.024
CATCGAT207.0334366E-445.024
GTGACGT207.0334366E-445.043
TCGACCG253.8909297E-545.045