##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545165_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1211044
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.054826249087565	31.0	31.0	33.0	30.0	34.0
2	31.329860847335027	31.0	31.0	34.0	30.0	34.0
3	31.432985093852906	31.0	31.0	34.0	30.0	34.0
4	35.21796648181239	37.0	35.0	37.0	33.0	37.0
5	35.06573336724347	37.0	35.0	37.0	32.0	37.0
6	35.10946340512814	37.0	35.0	37.0	32.0	37.0
7	35.74572435023005	37.0	35.0	37.0	35.0	37.0
8	35.75637549089876	37.0	35.0	37.0	35.0	37.0
9	37.46362394760224	39.0	37.0	39.0	35.0	39.0
10	36.64804086391576	39.0	35.0	39.0	32.0	39.0
11	36.261533850132615	38.0	35.0	39.0	31.0	39.0
12	35.23635639993262	37.0	35.0	39.0	30.0	39.0
13	34.734144259002974	37.0	33.0	39.0	27.0	39.0
14	35.62359749109033	37.0	33.0	40.0	27.0	41.0
15	36.06108944018549	38.0	34.0	40.0	30.0	41.0
16	36.28732234336655	37.0	34.0	40.0	31.0	41.0
17	36.2353729509415	37.0	34.0	40.0	31.0	41.0
18	36.17135298139456	37.0	35.0	40.0	31.0	41.0
19	36.102997083508114	37.0	35.0	40.0	30.0	41.0
20	35.92508034390163	36.0	34.0	40.0	30.0	41.0
21	35.777104712958405	36.0	34.0	40.0	30.0	41.0
22	35.667736267220675	36.0	34.0	40.0	30.0	41.0
23	35.65094249259317	36.0	34.0	40.0	30.0	41.0
24	35.547122152456886	36.0	34.0	40.0	30.0	41.0
25	35.48824980760402	36.0	34.0	40.0	30.0	41.0
26	35.33134469102691	35.0	34.0	40.0	29.0	41.0
27	35.2585372620648	35.0	34.0	40.0	29.0	41.0
28	35.26170642850301	36.0	34.0	40.0	29.0	41.0
29	35.27582152258712	36.0	34.0	40.0	29.0	41.0
30	35.226019038119176	36.0	34.0	40.0	29.0	41.0
31	35.02210159168453	36.0	34.0	40.0	28.0	41.0
32	34.822736415852766	35.0	34.0	40.0	27.0	41.0
33	34.624568554074	35.0	33.0	40.0	26.0	41.0
34	34.49800750426905	35.0	33.0	40.0	25.0	41.0
35	34.35455689471233	35.0	33.0	40.0	25.0	41.0
36	34.13492821070085	35.0	33.0	40.0	23.0	41.0
37	34.05263970590664	35.0	33.0	40.0	23.0	41.0
38	34.06515370209505	35.0	33.0	40.0	24.0	41.0
39	34.02445906176819	35.0	33.0	40.0	23.0	41.0
40	33.89982857765696	35.0	33.0	40.0	23.0	41.0
41	33.868785114331104	35.0	33.0	40.0	23.0	41.0
42	33.81926007642992	35.0	33.0	40.0	23.0	41.0
43	33.752086629387534	35.0	33.0	40.0	23.0	41.0
44	33.65265341308821	35.0	33.0	39.0	23.0	41.0
45	33.57587750734077	35.0	33.0	39.0	23.0	41.0
46	33.52644660309617	35.0	33.0	39.0	23.0	41.0
47	33.44222010100376	35.0	32.0	39.0	23.0	41.0
48	33.39970719478401	35.0	32.0	39.0	23.0	41.0
49	33.403556765897854	35.0	32.0	39.0	23.0	41.0
50	33.31462192950875	35.0	32.0	39.0	23.0	41.0
51	33.11902953154468	35.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	0.0
11	1.0
12	2.0
13	6.0
14	17.0
15	48.0
16	161.0
17	404.0
18	912.0
19	1747.0
20	2880.0
21	4674.0
22	6820.0
23	9585.0
24	13537.0
25	17974.0
26	23207.0
27	26624.0
28	29868.0
29	33330.0
30	40330.0
31	48755.0
32	60350.0
33	76923.0
34	125754.0
35	180707.0
36	83416.0
37	99317.0
38	131487.0
39	192070.0
40	136.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.33144295335265	23.886497930711023	29.582905327964966	14.199153787971369
2	31.006222730140276	24.98406333708767	31.255511773312943	12.75420215945911
3	27.708737254798336	26.28153890362365	32.86354583318195	13.146178008396062
4	25.130465945085394	27.76530002212967	32.424007715656906	14.680226317128032
5	22.388864483866815	33.49424133227199	30.369086507178928	13.747807676682267
6	19.635867895799	41.28107649267904	28.268089351006235	10.814966260515721
7	87.70003402023379	4.266566697824357	6.4055476101611495	1.6278516717807117
8	89.25447795455823	3.025736472002669	6.108035711336665	1.6117498621024504
9	85.48888397118519	4.597685963515776	7.5461337490627916	2.3672963162362395
10	51.28418125187855	25.763803792430334	12.93099177238812	10.021023183303
11	46.853541242101855	19.88466149867387	21.646364624241563	11.615432634982708
12	43.53888050310311	21.586168628059756	22.189036897090446	12.68591397174669
13	21.220203394756922	43.52665964242422	21.34827471173632	13.904862251082536
14	14.316077698250435	43.94299463933598	26.153880453559076	15.58704720885451
15	12.374612317966978	24.386810058098632	47.83509104541206	15.403486578522333
16	14.232017994391615	18.620050138558135	46.21698303282127	20.93094883422898
17	14.754294641648032	19.880450256142634	29.211655398152338	36.153599704056994
18	20.218918552917977	23.30964027731445	34.87990527181506	21.59153589795251
19	28.145385303919596	25.40593075065811	24.047185733961772	22.401498211460524
20	30.767750800136078	22.65565908422815	25.95619977473981	20.620390340895955
21	20.385469066359274	30.51235132662397	27.09505187259918	22.00712773441758
22	22.59158213904697	26.007973285859144	23.931913291342017	27.46853128375187
23	18.31097796611849	31.270870422544515	24.66689897311741	25.751252638219587
24	17.696879717004503	23.978649826100458	41.08422154768943	17.24024890920561
25	16.14549099784979	27.22204973560003	36.18431700252014	20.448142264030047
26	15.93575460511757	35.091953719270315	27.25433592833951	21.7179557472726
27	16.007263154765642	36.35722566644977	27.237243238065666	20.398267940718917
28	14.378090308857484	29.92533714712265	37.602101988036765	18.094470555983104
29	14.436387117231083	24.201680533490112	38.29258061639379	23.069351732885014
30	18.18199834192647	32.103953283282856	30.53060004425933	19.183448330531345
31	28.483193013631215	29.8341761323288	22.67506382922503	19.007567024814954
32	29.46556855077107	26.705883518683056	25.326082289330525	18.50246564121535
33	27.208507700793692	28.825707406171862	22.89809453661469	21.06769035641975
34	18.407836544336952	28.318211394466264	27.587436955222106	25.686515105974678
35	19.08766320629143	26.494743378440422	30.681709335086094	23.735884080182057
36	30.004442448003545	26.66178933217951	24.560792176006817	18.772976043810132
37	18.863724191689155	34.41509969910259	28.380802018754064	18.340374090454187
38	19.210202106612144	33.94979868609233	25.37331426438676	21.466684942908763
39	19.4334805341507	33.99826926189304	26.72677458457331	19.841475619382944
40	24.75690396054974	26.701176835854024	24.241315757313526	24.30060344628271
41	17.169566093387193	25.01486320893378	27.95009925320632	29.865471444472703
42	20.358302423363643	26.21217726193268	24.518927470843337	28.91059284386034
43	20.958032903841644	26.884489746037303	26.365103167184678	25.792374182936374
44	18.42005740501584	30.11005380481634	28.458586145507514	23.011302644660308
45	15.693484299496962	39.74653274364928	22.27161028005589	22.28837267679787
46	21.045890983316873	34.708235208629915	25.14764120874221	19.098232599311007
47	19.42134224685478	29.214050026258338	27.523855450338715	23.84075227654817
48	20.77843579589181	26.19591030548849	31.40257496837439	21.62307893024531
49	19.998695340549148	26.40102258877464	31.645258140909828	21.955023929766384
50	19.48475860497224	33.52206856233134	26.89216907065309	20.10100376204333
51	17.600103712169005	34.529876701424556	24.143879165414305	23.726140420992138
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1013.0
1	1254.5
2	1496.0
3	5143.0
4	8790.0
5	6537.5
6	4285.0
7	4641.0
8	4997.0
9	5568.0
10	6139.0
11	6556.5
12	6974.0
13	6992.5
14	7011.0
15	6672.5
16	6334.0
17	5996.5
18	5659.0
19	5282.0
20	4905.0
21	5068.5
22	5232.0
23	5203.0
24	5174.0
25	6568.5
26	8936.5
27	9910.0
28	12425.0
29	14940.0
30	16375.5
31	17811.0
32	22122.0
33	26433.0
34	31862.5
35	37292.0
36	37661.5
37	38031.0
38	47674.0
39	57317.0
40	73054.5
41	88792.0
42	109790.5
43	130789.0
44	127102.5
45	123416.0
46	112688.5
47	101961.0
48	99246.5
49	96532.0
50	91485.0
51	86438.0
52	78035.5
53	69633.0
54	62972.0
55	56311.0
56	50883.0
57	45455.0
58	39126.5
59	32798.0
60	30986.0
61	29174.0
62	25975.0
63	22776.0
64	19320.0
65	15864.0
66	13196.5
67	10529.0
68	9278.5
69	8028.0
70	7101.0
71	6174.0
72	5006.5
73	3839.0
74	2945.5
75	1806.5
76	1561.0
77	1084.0
78	607.0
79	464.0
80	321.0
81	235.0
82	149.0
83	127.0
84	105.0
85	61.0
86	17.0
87	13.0
88	9.0
89	6.5
90	4.0
91	3.0
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1211044.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.683548425088947
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.01608301252233	14.94984361750398
2	7.577399996727468	2.8314543955025298
3	2.799651356385834	1.5692226507120206
4	1.3863710874434985	1.036093253895753
5	0.8387672859812529	0.7835574602505585
6	0.5746958323953418	0.6442414448553101
7	0.43288368443077374	0.5661462296345299
8	0.3732056353228825	0.5578244448056929
9	0.2731030218752721	0.4592280180820295
>10	3.3734262993961295	14.571662857835289
>50	0.9935932045992169	13.279481833930154
>100	1.350188179928402	47.05666170533217
>500	0.009745452742265367	1.065745227975479
>1k	4.4297512464842577E-4	0.17719659339096847
>5k	4.4297512464842577E-4	0.45164026629356135
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5457	0.4506029508424137	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2141	0.17678961292901	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.027662083293422866	0.0
2	0.0	0.0	0.0	0.10098724736673481	0.0
3	0.0	0.0	0.0	0.14491628710434964	0.0
4	0.0	0.0	0.0	0.21749829073097263	0.0
5	0.0	0.0	0.0	0.374718011897173	0.0
6	0.0	0.0	0.0	0.5846195513953251	0.0
7	0.0	0.0	0.0	0.733334214116085	0.0
8	0.0	0.0	0.0	1.1469442893899808	0.0
9	8.257338296544139E-5	0.0	0.0	1.340991739358768	0.0
10	8.257338296544139E-5	0.0	0.0	1.6354484230135322	0.0
11	8.257338296544139E-5	0.0	0.0	1.8523687000637465	0.0
12	8.257338296544139E-5	0.0	0.0	2.0409663067568147	0.0
13	8.257338296544139E-5	0.0	0.0	2.1193284471910188	0.0
14	8.257338296544139E-5	0.0	0.0	2.1483117046118885	0.0
15	8.257338296544139E-5	0.0	0.0	2.189185529179782	0.0
16	8.257338296544139E-5	0.0	0.0	2.2872827081427265	0.0
17	8.257338296544139E-5	0.0	0.0	2.3944629592318694	0.0
18	8.257338296544139E-5	0.0	0.0	2.5743903607135663	0.0
19	8.257338296544139E-5	0.0	0.0	2.640944507383712	0.0
20	8.257338296544139E-5	0.0	0.0	2.71633400603116	0.0
21	8.257338296544139E-5	0.0	0.0	2.808073034505765	0.0
22	8.257338296544139E-5	0.0	0.0	2.90815197465988	0.0
23	8.257338296544139E-5	0.0	0.0	3.032920356320662	0.0
24	8.257338296544139E-5	0.0	0.0	3.1251548250930603	0.0
25	8.257338296544139E-5	0.0	0.0	3.198067122251545	0.0
26	8.257338296544139E-5	0.0	0.0	3.2683370711551354	0.0
27	8.257338296544139E-5	0.0	0.0	3.341166794930655	0.0
28	8.257338296544139E-5	0.0	0.0	3.4132533582594853	0.0
29	1.6514676593088279E-4	0.0	0.0	3.500946290968784	0.0
30	1.6514676593088279E-4	0.0	0.0	3.6214208567153627	0.0
31	1.6514676593088279E-4	0.0	0.0	3.718527155082722	0.0
32	1.6514676593088279E-4	0.0	0.0	3.8120827979825673	0.0
33	1.6514676593088279E-4	0.0	0.0	3.9063816013291013	0.0
34	1.6514676593088279E-4	0.0	0.0	4.0025795924838405	0.0
35	1.6514676593088279E-4	0.0	0.0	4.137256780100476	0.0
36	1.6514676593088279E-4	0.0	0.0	4.246996806061547	0.0
37	1.6514676593088279E-4	0.0	0.0	4.348974934023867	0.0
38	1.6514676593088279E-4	0.0	0.0	4.448888727412051	0.0
39	1.6514676593088279E-4	0.0	0.0	4.557472726011606	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGTA	35	1.2119926E-7	45.000004	21
GCGATAC	45	3.8562575E-10	45.000004	9
CGTAAGA	35	1.2119926E-7	45.000004	13
ACGTAAG	35	1.2119926E-7	45.000004	1
CACGTAC	35	1.2119926E-7	45.000004	22
GTGCATT	40	6.8157533E-9	45.0	9
TTGATAC	50	2.1827873E-11	45.0	12
ATCTCGT	25	3.8909297E-5	45.0	19
ACACGTC	20	7.0334366E-4	45.0	13
GGCGTAT	25	3.8909297E-5	45.0	8
TAGCGAA	20	7.0334366E-4	45.0	16
GTTAGCG	40	6.8157533E-9	45.0	1
CTAAGCG	25	3.8909297E-5	45.0	1
ATGTCGA	20	7.0334366E-4	45.0	25
ACTTACC	20	7.0334366E-4	45.0	12
CCGCTAA	25	3.8909297E-5	45.0	18
ACCACGT	20	7.0334366E-4	45.0	24
CATCGAT	20	7.0334366E-4	45.0	24
GTGACGT	20	7.0334366E-4	45.0	43
TCGACCG	25	3.8909297E-5	45.0	45
>>END_MODULE