FastQCFastQC Report
Thu 26 May 2016
SRR1545164_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545164_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1124164
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49250.43810333723549233No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC14040.12489280923423986No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAG600.045.0000041
ACGTTAG302.1654596E-645.0000041
ATTACGT302.1654596E-645.00000415
TGTTACG700.045.0000041
CCGAACT351.2119017E-745.00000412
CACGTAG302.1654596E-645.00000443
CGGTACT351.2119017E-745.00000437
TCGACCA302.1654596E-645.00000437
GTACGTC351.2119017E-745.0000049
AATCGGC302.1654596E-645.00000438
GACGTAA253.8907507E-545.039
ATCACGC207.03322E-445.011
TCTAGCG207.03322E-445.01
TACGCGG750.045.02
AACGCCA253.8907507E-545.026
ACGTAAG253.8907507E-545.01
CACGTTA253.8907507E-545.028
ATGTTCG253.8907507E-545.024
TACGTAC207.03322E-445.01
CTTACGA253.8907507E-545.021