##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1545164_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 1124164 Sequences flagged as poor quality 0 Sequence length 51 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.968684284499414 31.0 31.0 33.0 28.0 34.0 2 31.25157717201405 31.0 31.0 34.0 28.0 34.0 3 31.36709501460641 31.0 31.0 34.0 28.0 34.0 4 35.16308296654225 35.0 35.0 37.0 32.0 37.0 5 34.99692482591508 35.0 35.0 37.0 32.0 37.0 6 35.04610982027533 37.0 35.0 37.0 32.0 37.0 7 35.684850253165905 37.0 35.0 37.0 35.0 37.0 8 35.69194174515462 37.0 35.0 37.0 35.0 37.0 9 37.39725431520668 39.0 37.0 39.0 35.0 39.0 10 36.49810881686302 39.0 35.0 39.0 32.0 39.0 11 36.173702413526854 38.0 35.0 39.0 31.0 39.0 12 35.364276030899404 37.0 35.0 39.0 30.0 39.0 13 34.98098853903879 37.0 34.0 39.0 28.0 39.0 14 35.87598428699015 38.0 34.0 40.0 27.0 41.0 15 36.24687767976914 38.0 35.0 40.0 30.0 41.0 16 36.40448190833366 38.0 35.0 40.0 31.0 41.0 17 36.329086325482756 38.0 35.0 40.0 31.0 41.0 18 36.27201636060219 38.0 35.0 40.0 30.0 41.0 19 36.18941097562277 37.0 35.0 40.0 30.0 41.0 20 36.03657918239688 37.0 34.0 40.0 30.0 41.0 21 35.88908913646052 37.0 34.0 40.0 30.0 41.0 22 35.801184702587875 37.0 34.0 40.0 30.0 41.0 23 35.760756437672796 37.0 34.0 40.0 30.0 41.0 24 35.70722866058689 37.0 34.0 40.0 30.0 41.0 25 35.60826623161745 37.0 34.0 40.0 29.0 41.0 26 35.432955511829235 36.0 34.0 40.0 29.0 41.0 27 35.329203746072636 36.0 34.0 40.0 29.0 41.0 28 35.34726961546536 36.0 34.0 40.0 29.0 41.0 29 35.32133567700087 36.0 34.0 40.0 29.0 41.0 30 35.24230984091289 36.0 34.0 40.0 28.0 41.0 31 35.08584512580015 36.0 34.0 40.0 27.0 41.0 32 34.89588173967499 36.0 34.0 40.0 27.0 41.0 33 34.68292793578161 36.0 33.0 40.0 25.0 41.0 34 34.51417319892827 36.0 33.0 40.0 25.0 41.0 35 34.36197387569785 36.0 33.0 40.0 24.0 41.0 36 34.168624862564535 36.0 33.0 40.0 23.0 41.0 37 34.1423306563811 36.0 33.0 40.0 23.0 41.0 38 34.10619713849581 35.0 33.0 40.0 23.0 41.0 39 34.01440092370864 35.0 33.0 40.0 23.0 41.0 40 33.86391665273038 35.0 33.0 40.0 23.0 41.0 41 33.818353905657894 35.0 33.0 40.0 23.0 41.0 42 33.74808568856501 35.0 33.0 40.0 23.0 41.0 43 33.69550972989706 35.0 33.0 40.0 23.0 41.0 44 33.60130639301739 35.0 32.0 39.0 23.0 41.0 45 33.52722378585331 35.0 32.0 39.0 23.0 41.0 46 33.44565472653456 35.0 32.0 39.0 23.0 41.0 47 33.35690966798439 35.0 32.0 39.0 23.0 41.0 48 33.315067018691224 35.0 32.0 39.0 23.0 41.0 49 33.26912799200117 35.0 32.0 39.0 23.0 41.0 50 33.164989272027924 35.0 32.0 39.0 22.0 41.0 51 33.021923847410164 35.0 32.0 39.0 22.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 2.0 12 2.0 13 2.0 14 16.0 15 41.0 16 145.0 17 391.0 18 864.0 19 1634.0 20 2787.0 21 4555.0 22 6944.0 23 9732.0 24 13175.0 25 17466.0 26 21823.0 27 25101.0 28 28085.0 29 31967.0 30 37583.0 31 45348.0 32 55494.0 33 69930.0 34 108792.0 35 143769.0 36 85988.0 37 102225.0 38 132339.0 39 177856.0 40 108.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.186026238164544 22.630505869250396 28.76021648086934 14.423251411715729 2 30.803512654737208 25.871847879846715 30.470643073430566 12.853996391985511 3 28.1176056162624 26.20836461583897 32.85899566255458 12.815034105344061 4 26.157927135186682 27.54731516042143 31.702758672222203 14.591999032169683 5 23.240381296679132 31.344803783077914 30.979643539554726 14.435171380688228 6 19.424212125632913 41.796570607135614 28.013795140210856 10.765422127020614 7 87.0215555737419 4.074405513786244 7.252411569842122 1.6516273426297232 8 88.49616248163079 3.3010308104511443 6.247309111481955 1.9554975964361072 9 84.74946715959592 4.376051892784327 7.761412035966282 3.1130689116534596 10 49.351607060891475 23.397564768129918 15.143075209666918 12.107752961311695 11 43.106788689194815 22.26721368056618 20.96082066317726 13.665176967061745 12 38.905088581381364 21.283727285342707 24.181080340590874 15.630103792685054 13 23.428787970438478 34.8933073821969 24.94831714945506 16.729587497909556 14 16.551855423230062 37.438398667810034 28.6045452442882 17.405200664671703 15 15.358613156087547 22.51922317384296 43.92401820374963 18.19814546631986 16 17.831650897911693 18.542490241637342 41.577029686060044 22.048829174390924 17 18.69060030387025 20.57084197679342 28.517191441818095 32.22136627751823 18 21.834180777893618 23.58721681178191 32.91788386747841 21.660718542846062 19 28.47902975010764 24.7493248316082 25.089755587263067 21.6818898310211 20 30.62506894011906 23.66976704466608 25.100786006312248 20.604378008902614 21 22.728890090769674 28.996214075526343 28.193928999683322 20.080966834020657 22 22.550624286136188 23.430567070285118 26.38236058084052 27.63644806273818 23 21.04123597624546 29.273130966656108 25.363559053661213 24.322074003437223 24 19.68111414348796 23.818410836852983 37.77126824911668 18.729206770542376 25 19.392988923324356 25.282965830608344 33.460954095665755 21.86309115040154 26 18.13489846677175 31.841439505267914 27.526855512185055 22.496806515775276 27 18.877672652744618 32.35399817108536 27.29477193719066 21.473557238979364 28 17.26144939706306 27.394401528602586 34.792788240861654 20.551360833472696 29 17.660323582680107 24.34466857148957 34.67599033592963 23.319017509900693 30 20.378432328379134 29.69282062047886 29.903821862290553 20.024925188851448 31 28.170178016730656 25.655865158464426 25.67009795723756 20.503858867567367 32 29.93477819962212 23.973192523510807 25.23946683935796 20.852562437509118 33 28.041371187833803 26.01221885774673 24.62710067214392 21.31930928227554 34 19.400639052664914 25.78022423774467 27.956508125149 26.862628584441417 35 21.285506385189347 24.770051344821574 30.65246707775734 23.29197519223174 36 28.290445166363625 26.10179653502514 25.45829612049487 20.14946217811636 37 21.1306357435392 31.36828790105358 27.30304475147754 20.198031603929675 38 21.77013318341452 30.77068826256667 25.83840080272985 21.62077775128896 39 21.69283129507794 31.19269074619006 25.17666461477151 21.937813343960492 40 24.98550033624987 25.424582178401018 25.843471237292782 23.746446248056333 41 19.432929714881457 23.66158318537153 27.338270928441045 29.567216171305965 42 21.424187218235062 25.380905277165965 24.700844360787215 28.49406314381176 43 21.122451884244647 26.31938044626941 25.823189499041067 26.734978170444883 44 20.8231183350472 28.23840649584936 28.25068228479119 22.687792884312252 45 19.309282275539868 34.08906529652257 23.55163481484908 23.05001761308848 46 22.260097281179615 31.764048661939004 25.431965442764582 20.543888614116803 47 21.404883984899 26.562672350297646 27.245312961454022 24.787130703349334 48 22.3155162414025 24.91567066726919 30.697211438900375 22.071601652427937 49 22.12719852263549 24.51154813710455 31.168050213314068 22.19320312694589 50 19.966036983927612 31.5349895566839 26.623339655068122 21.875633804320366 51 19.32049060457371 31.418102696759547 25.360445628929583 23.900961069737157 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 587.0 1 932.0 2 1277.0 3 4815.5 4 8354.0 5 6177.5 6 4001.0 7 4224.0 8 4447.0 9 4800.0 10 5153.0 11 5270.0 12 5387.0 13 5377.5 14 5368.0 15 4986.0 16 4604.0 17 4391.0 18 4178.0 19 4300.0 20 4422.0 21 4132.5 22 3843.0 23 3913.0 24 3983.0 25 4704.0 26 6673.5 27 7922.0 28 8363.5 29 8805.0 30 11801.5 31 14798.0 32 18758.5 33 22719.0 34 24911.0 35 27103.0 36 30961.5 37 34820.0 38 38913.5 39 43007.0 40 57888.0 41 72769.0 42 86994.0 43 101219.0 44 99548.5 45 97878.0 46 97158.5 47 96439.0 48 91300.0 49 86161.0 50 82596.5 51 79032.0 52 74097.0 53 69162.0 54 62423.5 55 55685.0 56 54361.5 57 53038.0 58 51093.0 59 49148.0 60 44581.0 61 40014.0 62 35588.5 63 31163.0 64 27768.5 65 24374.0 66 19682.5 67 14991.0 68 12826.5 69 10662.0 70 9352.5 71 8043.0 72 6934.0 73 5825.0 74 5001.0 75 3309.5 76 2442.0 77 1660.0 78 878.0 79 691.0 80 504.0 81 360.0 82 216.0 83 153.5 84 91.0 85 64.0 86 37.0 87 24.0 88 11.0 89 5.5 90 0.0 91 0.0 92 0.0 93 1.0 94 2.0 95 1.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1124164.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.115437304155712 #Duplication Level Percentage of deduplicated Percentage of total 1 77.86575948661432 16.441695625800723 2 7.467021477776465 3.1533884772554623 3 2.8997885324300907 1.8369090885551185 4 1.668340154703976 1.40910927754629 5 1.0492002825426583 1.1077161392765982 6 0.7983554706098362 1.0114574931655034 7 0.6040252969635301 0.8927980801710219 8 0.5005060072578013 0.8454722573284326 9 0.4047421690655757 0.7691677105726941 >10 4.580986064049457 22.326331188388433 >50 1.182560576680875 17.802511195219218 >100 0.9766042732819039 31.689535747978177 >500 0.0012661248141079134 0.149892994997453 >1k 8.440832094052756E-4 0.5640147237448753 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4925 0.43810333723549233 No Hit GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC 1404 0.12489280923423986 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.027487092630612615 0.0 2 0.0 0.0 0.0 0.10558957589817856 0.0 3 0.0 0.0 0.0 0.1513124419568675 0.0 4 0.0 0.0 0.0 0.25031934842247217 0.0 5 0.0 0.0 0.0 0.4591856704181952 0.0 6 0.0 0.0 0.0 0.7444643308271747 0.0 7 0.0 0.0 0.0 0.9476375333136446 0.0 8 0.0 0.0 0.0 1.54114524215328 0.0 9 0.0 0.0 0.0 1.8070317142338663 0.0 10 0.0 0.0 0.0 2.216580498930761 0.0 11 0.0 0.0 0.0 2.441814539515587 0.0 12 0.0 0.0 0.0 2.680036008980896 0.0 13 0.0 0.0 0.0 2.7687241363359796 0.0 14 0.0 0.0 0.0 2.8043061332688115 0.0 15 0.0 0.0 0.0 2.8476272145345343 0.0 16 0.0 0.0 0.0 2.936493251874282 0.0 17 0.0 0.0 0.0 3.0413711878338034 0.0 18 0.0 0.0 0.0 3.2141217829427022 0.0 19 0.0 0.0 0.0 3.2828839920154 0.0 20 8.895499233207966E-5 0.0 0.0 3.361520205236958 0.0 21 8.895499233207966E-5 0.0 0.0 3.4490519176917247 0.0 22 8.895499233207966E-5 0.0 0.0 3.549926878996303 0.0 23 8.895499233207966E-5 0.0 0.0 3.66957134368295 0.0 24 8.895499233207966E-5 0.0 0.0 3.759860660900011 0.0 25 8.895499233207966E-5 0.0 0.0 3.8297792848730254 0.0 26 8.895499233207966E-5 0.0 0.0 3.9045904334243047 0.0 27 8.895499233207966E-5 0.0 0.0 3.9892755861244447 0.0 28 8.895499233207966E-5 0.0 0.0 4.068712394276992 0.0 29 8.895499233207966E-5 0.0 0.0 4.158912756501721 0.0 30 2.6686497699623897E-4 0.0 0.0 4.292878974953832 0.0 31 2.6686497699623897E-4 0.0 0.0 4.393664981266078 0.0 32 2.6686497699623897E-4 0.0 0.0 4.479862368835864 0.0 33 2.6686497699623897E-4 0.0 0.0 4.569351091121936 0.0 34 2.6686497699623897E-4 0.0 0.0 4.667824267633548 0.0 35 2.6686497699623897E-4 0.0 0.0 4.8001892962236825 0.0 36 2.6686497699623897E-4 0.0 0.0 4.898929337712291 0.0 37 2.6686497699623897E-4 0.0 0.0 4.999626389032206 0.0 38 2.6686497699623897E-4 0.0 0.0 5.113844599186596 0.0 39 2.6686497699623897E-4 0.0 0.0 5.288641159119132 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGTTAG 60 0.0 45.000004 1 ACGTTAG 30 2.1654596E-6 45.000004 1 ATTACGT 30 2.1654596E-6 45.000004 15 TGTTACG 70 0.0 45.000004 1 CCGAACT 35 1.2119017E-7 45.000004 12 CACGTAG 30 2.1654596E-6 45.000004 43 CGGTACT 35 1.2119017E-7 45.000004 37 TCGACCA 30 2.1654596E-6 45.000004 37 GTACGTC 35 1.2119017E-7 45.000004 9 AATCGGC 30 2.1654596E-6 45.000004 38 GACGTAA 25 3.8907507E-5 45.0 39 ATCACGC 20 7.03322E-4 45.0 11 TCTAGCG 20 7.03322E-4 45.0 1 TACGCGG 75 0.0 45.0 2 AACGCCA 25 3.8907507E-5 45.0 26 ACGTAAG 25 3.8907507E-5 45.0 1 CACGTTA 25 3.8907507E-5 45.0 28 ATGTTCG 25 3.8907507E-5 45.0 24 TACGTAC 20 7.03322E-4 45.0 1 CTTACGA 25 3.8907507E-5 45.0 21 >>END_MODULE