FastQCFastQC Report
Thu 26 May 2016
SRR1545163_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545163_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1302565
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44110.3386395304648904No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC17370.1333522703281602No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTATT207.033633E-445.00000443
CGAGTTA302.1657252E-645.00000438
TACGGTA302.1657252E-645.0000046
CGACAGT207.033633E-445.00000441
CGCCTAG207.033633E-445.00000437
CGCTAAG406.8157533E-945.00000415
ACTCGCT302.1657252E-645.00000410
CGACTAG207.033633E-445.00000416
TGCGTTA207.033633E-445.00000435
ACGTTAG1000.045.01
ATTAACG253.8910937E-545.01
CTAAGCG502.1827873E-1145.01
ACAATCG253.8910937E-545.018
GGACGTA351.2121018E-745.08
CGTACTG351.2121018E-745.011
CTCGACC253.8910937E-545.036
ACGTAAG351.2121018E-745.01
CAATCGA453.8562575E-1045.041
TACGTAG950.045.01
CTTACGA351.2121018E-745.044