FastQCFastQC Report
Thu 26 May 2016
SRR1545162_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545162_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1143977
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42780.37395856734881905No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC14800.12937323040585605No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT207.033271E-445.00000419
TAGCGGA207.033271E-445.0000043
ATCGTTA406.8157533E-945.00000423
CAAGGTA207.033271E-445.00000431
ACTACGC207.033271E-445.00000416
ACTACCG650.045.0000041
CGTGTCA207.033271E-445.00000442
ATGTTAC207.033271E-445.00000410
GTGCGTA207.033271E-445.00000440
GCCAATC207.033271E-445.00000440
GTCGGTT207.033271E-445.00000435
GTACGGA207.033271E-445.0000043
CGAAGTA207.033271E-445.00000414
GACTACG207.033271E-445.0000041
CGAACTA253.890794E-545.06
ACCGCAT253.890794E-545.037
CGCGACT453.8562575E-1045.025
TCTAGCG502.1827873E-1145.01
TAGCCGT351.2119199E-745.044
CACCTAG253.890794E-545.01