##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545162_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1143977
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.0768337125659	31.0	31.0	33.0	30.0	34.0
2	31.33824543675266	31.0	31.0	34.0	30.0	34.0
3	31.4545030188544	31.0	31.0	34.0	30.0	34.0
4	35.22592062602657	37.0	35.0	37.0	33.0	37.0
5	35.09021422633497	37.0	35.0	37.0	32.0	37.0
6	35.14711746827078	37.0	35.0	37.0	32.0	37.0
7	35.75118817948263	37.0	35.0	37.0	35.0	37.0
8	35.75420659681095	37.0	35.0	37.0	35.0	37.0
9	37.45988074935073	39.0	37.0	39.0	35.0	39.0
10	36.66474238555495	39.0	35.0	39.0	32.0	39.0
11	36.262006141731874	38.0	35.0	39.0	31.0	39.0
12	35.178850623745056	37.0	35.0	39.0	30.0	39.0
13	34.67030980517965	37.0	33.0	39.0	27.0	39.0
14	35.52631215487724	37.0	33.0	40.0	27.0	41.0
15	35.92289268053466	37.0	34.0	40.0	30.0	41.0
16	36.13894510116899	37.0	34.0	40.0	31.0	41.0
17	36.06585272256348	37.0	34.0	40.0	31.0	41.0
18	36.004226483574406	37.0	34.0	40.0	30.0	41.0
19	35.92072830135571	37.0	34.0	40.0	30.0	41.0
20	35.78949139711725	36.0	34.0	40.0	30.0	41.0
21	35.61830788556064	36.0	34.0	40.0	30.0	41.0
22	35.5067794195163	36.0	34.0	40.0	29.0	41.0
23	35.50720862394961	36.0	34.0	40.0	29.0	41.0
24	35.44882807958552	35.0	34.0	40.0	29.0	41.0
25	35.33310110255713	35.0	34.0	40.0	29.0	41.0
26	35.17608395972996	35.0	34.0	40.0	29.0	41.0
27	35.06676270589356	35.0	34.0	40.0	29.0	41.0
28	35.09855268069201	36.0	34.0	40.0	29.0	41.0
29	35.09791980083516	36.0	34.0	40.0	29.0	41.0
30	35.02711155906106	36.0	34.0	40.0	29.0	41.0
31	34.83110149941826	35.0	34.0	40.0	27.0	41.0
32	34.637687645818055	35.0	33.0	40.0	27.0	41.0
33	34.46626986381719	35.0	33.0	40.0	26.0	41.0
34	34.334572285981274	35.0	33.0	40.0	25.0	41.0
35	34.18660777270872	35.0	33.0	40.0	24.0	41.0
36	33.969294837221376	35.0	33.0	40.0	23.0	41.0
37	33.92476072508451	35.0	33.0	40.0	23.0	41.0
38	33.90646490270346	35.0	33.0	40.0	23.0	41.0
39	33.85896482184519	35.0	33.0	39.0	23.0	41.0
40	33.71275034375691	35.0	33.0	39.0	23.0	41.0
41	33.692197482991354	35.0	33.0	39.0	23.0	41.0
42	33.61939269758046	35.0	33.0	39.0	23.0	41.0
43	33.5330351921411	35.0	33.0	39.0	23.0	41.0
44	33.424604690478915	35.0	32.0	39.0	23.0	41.0
45	33.341307561253416	35.0	32.0	39.0	23.0	41.0
46	33.28403630492571	35.0	32.0	39.0	23.0	41.0
47	33.22634371145574	35.0	32.0	39.0	23.0	41.0
48	33.15635191966272	35.0	32.0	38.0	23.0	40.0
49	33.1749947769929	35.0	32.0	38.0	23.0	40.0
50	33.0935919166207	35.0	32.0	38.0	23.0	40.0
51	32.9269312232676	35.0	32.0	38.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	1.0
13	4.0
14	15.0
15	40.0
16	143.0
17	377.0
18	863.0
19	1645.0
20	2783.0
21	4476.0
22	6777.0
23	9524.0
24	13078.0
25	17387.0
26	22085.0
27	25771.0
28	29340.0
29	33315.0
30	39571.0
31	47684.0
32	58792.0
33	74846.0
34	124154.0
35	170541.0
36	80887.0
37	93804.0
38	122364.0
39	163583.0
40	125.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.49604056724917	23.616820967554418	29.510121269920635	13.377017195275778
2	30.573429360904985	26.534012484516733	30.601576779952744	12.290981374625538
3	28.60957868908204	25.008282509176322	32.67364641072329	13.708492391018352
4	25.66616286865907	27.797324596560948	31.86550079241104	14.671011742368947
5	23.33989232300999	32.47906207904529	30.261534978413028	13.919510619531685
6	20.48747483559547	41.603458810797775	26.557177285906974	11.351889067699787
7	87.60744315663689	4.318793122589002	6.421020702339296	1.6527430184348113
8	89.27373539852637	3.0531208232333342	5.968826296332881	1.704317481907416
9	85.15949184293041	4.912336524248302	7.295164151027513	2.633007481793777
10	54.463070498795	23.404928595592395	11.536857821442215	10.595143084170399
11	49.988767256684355	18.299668612218603	18.376680650048034	13.334883481049006
12	44.10167337280382	20.427770838050066	21.507949897594095	13.962605891552016
13	23.53124232392784	38.578922478336544	22.041876716052858	15.847958481682761
14	16.603917736108333	40.7650678291609	27.509993644977126	15.12102078975364
15	15.57732366996889	20.994652864524372	47.187137503638624	16.240885961868116
16	18.215576012454797	17.455246040785784	44.78726407960999	19.541913867149425
17	18.055345518310244	18.41418140399676	27.799509955182668	35.73096312251033
18	23.229837662820145	21.920283362340324	33.68817729727084	21.161701677568693
19	30.923436397759748	24.276537028279417	24.397693310267602	20.40233326369324
20	33.60102519543663	23.25903405400633	23.63351710742436	19.506423643132685
21	24.139995821594315	28.925581545782826	26.302626713648962	20.631795918973893
22	24.057826337417623	23.38823245572245	24.922703865549746	27.631237341310182
23	21.49343911634587	29.855407932152485	23.73832690692208	24.912826044579568
24	21.562146791412765	22.53515586414762	38.01754755558896	17.885149788850647
25	18.917775444786038	23.590072178024556	35.15700053410165	22.335151843087754
26	17.82570803433985	32.96263823485962	26.164949120480568	23.046704610319964
27	18.46155997891566	32.82635927120912	27.354570939800364	21.357509810074855
28	16.47568089218577	29.092193287102802	36.850391222900456	17.581734597810968
29	18.110241726887864	23.937369370188385	36.480191472381	21.472197430542746
30	20.14428611764048	29.571660968708287	30.748957365401573	19.535095548249657
31	31.97284560790995	24.631264439757093	24.083700983498794	19.312188968834164
32	32.53579398886516	25.089577849904327	24.735025267116384	17.63960289411413
33	30.06188061473264	26.4432763945429	23.33097605983337	20.163866930891093
34	20.39918634727796	27.511567103184763	27.671972426019053	24.41727412351822
35	21.495362231932987	25.102864830324386	31.05857897492694	22.343193962815686
36	31.0174942328386	26.819595149203174	24.108177000062064	18.054733617896165
37	21.917923175028868	33.31098439916187	26.028495328140338	18.742597097668924
38	22.132787634716433	34.1721905248095	21.35086631986482	22.34415552060924
39	22.706575394435376	31.486559607404697	23.908697465071413	21.898167533088515
40	25.619396194154255	24.340436914378525	23.71594883463566	26.324218056831562
41	18.44661212594309	24.125222797311487	25.598678994420343	31.829486082325083
42	22.121511184228353	23.953103952264772	23.516032227920665	30.409352635586206
43	22.086283203246218	25.05059105209283	24.198388603966688	28.664737140694264
44	19.922778167742884	28.45048458142078	27.990947370445383	23.635789880390952
45	18.543379805712878	35.93726097640075	22.904743714252994	22.614615503633377
46	22.185148827292856	32.48570556925532	25.004523692346964	20.324621911104856
47	22.262160865122286	26.492403256359175	25.763717277532677	25.481718600985857
48	22.92764627260863	24.18929751210033	30.63435715927855	22.248699056012487
49	22.83822139780782	23.486486179355005	31.093282469839867	22.582009952997307
50	19.884053612965996	32.113407874459014	25.059070243545108	22.943468269029886
51	19.065243444579743	33.43266516721927	23.95625086868005	23.545840519520933
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	944.0
1	1313.0
2	1682.0
3	5059.0
4	8436.0
5	6269.5
6	4103.0
7	4270.5
8	4438.0
9	4808.0
10	5178.0
11	5317.5
12	5457.0
13	5428.0
14	5399.0
15	5057.5
16	4716.0
17	4468.5
18	4221.0
19	4101.0
20	3981.0
21	3871.5
22	3762.0
23	3798.5
24	3835.0
25	5074.5
26	7008.0
27	7702.0
28	9536.5
29	11371.0
30	11906.5
31	12442.0
32	14450.5
33	16459.0
34	18417.5
35	20376.0
36	23103.5
37	25831.0
38	32254.0
39	38677.0
40	50936.5
41	63196.0
42	85563.5
43	107931.0
44	110006.0
45	112081.0
46	103301.5
47	94522.0
48	91087.0
49	87652.0
50	85478.0
51	83304.0
52	76057.0
53	68810.0
54	64800.5
55	60791.0
56	59474.5
57	58158.0
58	55607.0
59	53056.0
60	50012.0
61	46968.0
62	41116.5
63	35265.0
64	30850.5
65	26436.0
66	21512.0
67	16588.0
68	13717.5
69	10847.0
70	9412.0
71	7977.0
72	6740.5
73	5504.0
74	4714.0
75	3427.0
76	2930.0
77	2191.5
78	1453.0
79	1125.0
80	797.0
81	529.5
82	262.0
83	178.5
84	95.0
85	77.0
86	59.0
87	41.5
88	24.0
89	17.0
90	10.0
91	10.0
92	10.0
93	6.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1143977.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.8501967409562
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.98730205970443	14.277890785440153
2	7.120747367621226	2.542134829093697
3	2.516987913890794	1.347861883726788
4	1.249446079341536	0.8921143333385115
5	0.8185959140089152	0.7306049058200998
6	0.5671962243555797	0.6074738517284779
7	0.4286451144034719	0.5355979736906156
8	0.36164075870086715	0.5164286953887318
9	0.2780397810035698	0.44667583134534883
>10	3.999930573173656	16.85966753712133
>50	1.2476016782312689	15.950654997853588
>100	1.4165017086565594	44.1027104995052
>500	0.00638284998701396	0.6855389011532509
>1k	9.819769210790707E-4	0.5046449747942238
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4278	0.37395856734881905	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1480	0.12937323040585605	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.741434486882166E-5	0.0	0.0	0.02526274566708946	0.0
2	8.741434486882166E-5	0.0	0.0	0.09939011011585024	0.0
3	8.741434486882166E-5	0.0	0.0	0.13619154930562416	0.0
4	8.741434486882166E-5	0.0	0.0	0.19991660671499514	0.0
5	8.741434486882166E-5	0.0	0.0	0.34572373395618966	0.0
6	8.741434486882166E-5	0.0	0.0	0.5074402719635097	0.0
7	8.741434486882166E-5	0.0	0.0	0.6196802907750768	0.0
8	8.741434486882166E-5	0.0	0.0	0.8919759750414562	0.0
9	8.741434486882166E-5	0.0	0.0	1.011646213166873	0.0
10	8.741434486882166E-5	0.0	0.0	1.2120873059510813	0.0
11	8.741434486882166E-5	0.0	0.0	1.3786990472710552	0.0
12	8.741434486882166E-5	0.0	0.0	1.5259922183750199	0.0
13	1.7482868973764333E-4	0.0	0.0	1.582199642125672	0.0
14	1.7482868973764333E-4	0.0	0.0	1.6030042562044517	0.0
15	1.7482868973764333E-4	0.0	0.0	1.6375329224276363	0.0
16	1.7482868973764333E-4	0.0	0.0	1.7158561754301005	0.0
17	1.7482868973764333E-4	0.0	0.0	1.805805536300118	0.0
18	1.7482868973764333E-4	0.0	0.0	1.9488154045055102	0.0
19	1.7482868973764333E-4	0.0	0.0	2.010355103293161	0.0
20	1.7482868973764333E-4	0.0	0.0	2.072943774219237	0.0
21	1.7482868973764333E-4	0.0	0.0	2.151878927635783	0.0
22	1.7482868973764333E-4	0.0	0.0	2.229765108913903	0.0
23	1.7482868973764333E-4	0.0	0.0	2.3251341591657875	0.0
24	1.7482868973764333E-4	0.0	0.0	2.392006132990436	0.0
25	1.7482868973764333E-4	0.0	0.0	2.450923401432022	0.0
26	1.7482868973764333E-4	0.0	0.0	2.5142113871170486	0.0
27	1.7482868973764333E-4	0.0	0.0	2.5706810539023075	0.0
28	1.7482868973764333E-4	0.0	0.0	2.6304724657925815	0.0
29	1.7482868973764333E-4	0.0	0.0	2.6927988936840515	0.0
30	1.7482868973764333E-4	0.0	0.0	2.781262210691299	0.0
31	1.7482868973764333E-4	0.0	0.0	2.8642184239718107	0.0
32	1.7482868973764333E-4	0.0	0.0	2.932751270348967	0.0
33	1.7482868973764333E-4	0.0	0.0	3.006791220452859	0.0
34	1.7482868973764333E-4	0.0	0.0	3.082579457454127	0.0
35	1.7482868973764333E-4	0.0	0.0	3.181095424121289	0.0
36	1.7482868973764333E-4	0.0	0.0	3.2710447849913065	0.0
37	1.7482868973764333E-4	0.0	0.0	3.3564485999281453	0.0
38	1.7482868973764333E-4	0.0	0.0	3.4437755304520983	0.0
39	1.7482868973764333E-4	0.0	0.0	3.5407180389116215	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCGT	20	7.033271E-4	45.000004	19
TAGCGGA	20	7.033271E-4	45.000004	3
ATCGTTA	40	6.8157533E-9	45.000004	23
CAAGGTA	20	7.033271E-4	45.000004	31
ACTACGC	20	7.033271E-4	45.000004	16
ACTACCG	65	0.0	45.000004	1
CGTGTCA	20	7.033271E-4	45.000004	42
ATGTTAC	20	7.033271E-4	45.000004	10
GTGCGTA	20	7.033271E-4	45.000004	40
GCCAATC	20	7.033271E-4	45.000004	40
GTCGGTT	20	7.033271E-4	45.000004	35
GTACGGA	20	7.033271E-4	45.000004	3
CGAAGTA	20	7.033271E-4	45.000004	14
GACTACG	20	7.033271E-4	45.000004	1
CGAACTA	25	3.890794E-5	45.0	6
ACCGCAT	25	3.890794E-5	45.0	37
CGCGACT	45	3.8562575E-10	45.0	25
TCTAGCG	50	2.1827873E-11	45.0	1
TAGCCGT	35	1.2119199E-7	45.0	44
CACCTAG	25	3.890794E-5	45.0	1
>>END_MODULE