FastQCFastQC Report
Thu 26 May 2016
SRR1545161_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545161_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3180488
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT405631.2753703205294282No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT348050.043.9785961
TATCGCG950.042.631581
CGACGGT8800.041.67613228
CGGTCTA8750.041.65714331
GTCGGTA556.184564E-1140.9090911
TCTAGCG1250.039.6000021
GTTTTTT395400.039.4290582
TCACGAC9450.039.28571725
AGGGCGA27500.039.272736
ACGGGAT7450.039.2617455
TAGGGCG13300.039.248125
GCGTAAG1950.039.230771
GCGATAC1550.039.1935469
GGCGATA11650.038.8197448
ACGGTCT9400.038.53723530
ACGACGG9650.038.23834227
GGGCGAT49500.038.1363647
GTAGGGT16150.037.8947374
TAGGGTA16450.037.887545
TACGTAG1550.037.7419361
Produced by FastQC (version 0.11.5)