##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545161_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3180488
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.02635979132762	31.0	31.0	33.0	28.0	34.0
2	31.35068612112355	31.0	31.0	34.0	30.0	34.0
3	31.368468612363888	31.0	31.0	34.0	28.0	34.0
4	35.19502604631742	37.0	35.0	37.0	32.0	37.0
5	35.076280118019625	37.0	35.0	37.0	32.0	37.0
6	35.13289124184716	37.0	35.0	37.0	32.0	37.0
7	35.75044552911377	37.0	35.0	37.0	35.0	37.0
8	35.75024901838963	37.0	35.0	37.0	35.0	37.0
9	37.46068087664535	39.0	37.0	39.0	35.0	39.0
10	36.6247082208768	39.0	35.0	39.0	32.0	39.0
11	36.33326803937006	38.0	35.0	39.0	31.0	39.0
12	35.98735036887421	38.0	35.0	39.0	31.0	39.0
13	35.84017515551073	38.0	35.0	39.0	30.0	39.0
14	36.81805276422989	39.0	35.0	41.0	31.0	41.0
15	36.97954464849419	39.0	35.0	41.0	31.0	41.0
16	37.01644433181323	39.0	35.0	41.0	31.0	41.0
17	36.930289942927	39.0	35.0	41.0	31.0	41.0
18	36.72763047683249	38.0	35.0	40.0	31.0	41.0
19	36.57380030988955	38.0	35.0	40.0	31.0	41.0
20	36.359395476417454	38.0	35.0	40.0	30.0	41.0
21	36.25687127258458	38.0	35.0	40.0	30.0	41.0
22	36.19114362324272	38.0	35.0	40.0	30.0	41.0
23	36.09815726391673	38.0	34.0	40.0	30.0	41.0
24	35.99259704800018	38.0	34.0	40.0	30.0	41.0
25	35.889769746026396	37.0	34.0	40.0	30.0	41.0
26	35.77131905544055	37.0	34.0	40.0	30.0	41.0
27	35.676784191608334	37.0	34.0	40.0	29.0	41.0
28	35.57964878345713	37.0	34.0	40.0	29.0	41.0
29	35.49256623511864	37.0	34.0	40.0	29.0	41.0
30	35.34469615983459	37.0	34.0	40.0	27.0	41.0
31	35.09273419676477	37.0	34.0	40.0	26.0	41.0
32	34.7696384957277	37.0	33.0	40.0	24.0	41.0
33	34.384852576082665	37.0	33.0	40.0	23.0	41.0
34	34.02634910114423	37.0	33.0	40.0	21.0	41.0
35	33.72899347521513	37.0	33.0	40.0	18.0	41.0
36	33.564193922442094	37.0	33.0	40.0	18.0	41.0
37	33.50624778335903	37.0	32.0	40.0	18.0	41.0
38	33.38663249161764	37.0	32.0	40.0	18.0	41.0
39	33.291744222899126	37.0	32.0	40.0	17.0	41.0
40	33.19458932088409	37.0	32.0	40.0	16.0	41.0
41	33.06619455882242	36.0	32.0	40.0	15.0	41.0
42	32.98996286104522	36.0	31.0	40.0	15.0	41.0
43	32.93606672938241	36.0	31.0	40.0	15.0	41.0
44	32.79866202922319	36.0	31.0	40.0	15.0	41.0
45	32.74111079809136	36.0	31.0	40.0	15.0	41.0
46	32.675824904857365	36.0	31.0	40.0	15.0	41.0
47	32.590458130953486	35.0	31.0	40.0	15.0	41.0
48	32.49541579782725	35.0	31.0	40.0	15.0	41.0
49	32.443820256514094	35.0	31.0	39.0	15.0	41.0
50	32.40619332630716	35.0	31.0	39.0	15.0	41.0
51	32.255625237384955	35.0	31.0	39.0	14.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	2.0
12	3.0
13	10.0
14	49.0
15	156.0
16	544.0
17	1556.0
18	3471.0
19	6322.0
20	10469.0
21	15984.0
22	23160.0
23	32684.0
24	46525.0
25	64632.0
26	84213.0
27	92319.0
28	91662.0
29	93415.0
30	102298.0
31	117110.0
32	138544.0
33	169865.0
34	239512.0
35	294683.0
36	254786.0
37	308565.0
38	413832.0
39	573879.0
40	236.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.541724414618137	22.90352926972213	27.0118296311761	20.54291668448364
2	36.85805448723592	23.23228385078013	27.20997532454139	12.699686337442555
3	26.736966151106373	24.183049896745406	36.90043792021853	12.179546031929691
4	24.06797321668876	25.696654098364778	36.504618159225885	13.730754525720581
5	20.724335385010097	30.292584031129817	35.87575240026059	13.107328183599499
6	18.883265712683084	39.002379509056475	32.580409044146684	9.53394573411376
7	82.22659541554629	3.2000120736188915	13.27808814244858	1.295304368386235
8	83.58833612954993	2.149481463221996	12.922953961781966	1.3392284454461076
9	79.22881645835483	4.12465005370245	14.127863397063594	2.5186700908791355
10	36.88974773682529	27.105714594741436	21.1222931826814	14.882244485751873
11	28.385298105196433	23.668223241213298	30.07893757184432	17.867541081745948
12	27.636167783057193	22.00420187090786	33.00496653343764	17.354663812597312
13	22.82093188215142	25.142179439130096	34.57481996473497	17.462068713983513
14	19.27163378701633	27.45182500295552	35.522630489409174	17.753910720618972
15	18.059335548507022	23.51799472282241	40.19483802485656	18.227831703814008
16	21.07673413639668	21.75150480052118	38.42136804163386	18.750393021448282
17	21.24686526092851	21.1602433337274	34.88332608077754	22.709565324566544
18	21.924308470901323	22.746855199579436	36.06248475076781	19.266351578751436
19	24.73626688734559	23.49205530723587	32.664641400942244	19.107036404476293
20	25.16359124763244	23.71570023216563	32.21401872920131	18.906689791000627
21	23.24344565991131	24.403361999793745	34.25166200910049	18.101530331194457
22	22.510413496293648	21.880227185262136	34.21984299264767	21.389516325796546
23	19.87842746144617	24.822417188808764	34.5520876041664	20.747067745578665
24	19.342157555695856	24.402513073465457	37.1829731789587	19.072356191879987
25	19.984417485618557	24.892563656897934	35.12105689441369	20.001961963069817
26	19.13929560495119	27.08527747943083	32.88086608092846	20.894560834689518
27	18.65267216854772	25.76217863422217	35.21164047781347	20.373508719416645
28	18.83333626789348	24.792484675307687	37.168038363924026	19.206140692874804
29	19.40249420843594	24.50372395682675	36.488425675556705	19.605356159180605
30	20.576590762172344	25.14107897907491	35.095557662849224	19.186772595903523
31	23.417192581767328	24.743089739687747	32.74723878851296	19.09247889003197
32	23.32107525637575	25.09250152806739	32.68250029555213	18.903922920004728
33	22.82960979572946	25.49225150354285	31.57043824721238	20.10770045351531
34	20.192058577174322	26.394377215068882	32.40942899328657	21.00413521447023
35	20.207716551673833	26.461914020741474	32.31167669867014	21.018692728914555
36	23.101014687054313	27.208308913600682	29.497548803831364	20.193127595513644
37	20.629129869378534	29.683778086884782	29.29713930692397	20.389952736812717
38	21.177882136326247	29.05371754271672	28.87034945580678	20.898050865150253
39	20.838185838148107	28.138323427096722	28.705028913801904	22.31846182095326
40	22.274757835904428	26.66980664602413	29.610644655788672	21.44479086228277
41	20.292703509650092	26.280023694477073	29.846300316177892	23.58097247969494
42	21.058435057764722	27.65581885547124	28.530558832481052	22.75518725428299
43	20.996431994083927	27.41538405427092	29.291605564932173	22.29657838671298
44	21.388132890298593	27.77633495237209	29.6364268627959	21.199105294533418
45	20.324428200955325	28.925089483123344	29.215107870238782	21.53537444568255
46	21.032086899871967	28.4665120572692	29.796842497126224	20.704558545732603
47	20.557757174370725	27.949547365058443	30.058594781681304	21.43410067888953
48	20.689655172413794	27.36149295328264	30.807064827787435	21.141787046516132
49	21.307736422838257	26.301561269842864	31.462907578962724	20.927794728356155
50	20.4859757370567	27.990956104849317	30.68843523383833	20.83463292425565
51	20.026738035169444	28.786557283033293	30.212753514554997	20.973951167242262
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1533.0
1	2631.5
2	3730.0
3	38205.0
4	72680.0
5	53557.0
6	34434.0
7	34545.5
8	34657.0
9	35298.0
10	35939.0
11	35913.0
12	35887.0
13	35040.5
14	34194.0
15	32388.0
16	30582.0
17	28666.0
18	26750.0
19	25220.5
20	23691.0
21	22785.5
22	21880.0
23	21975.0
24	22070.0
25	23791.5
26	28441.5
27	31370.0
28	36641.5
29	41913.0
30	48781.0
31	55649.0
32	62014.5
33	68380.0
34	81670.0
35	94960.0
36	101463.0
37	107966.0
38	121193.5
39	134421.0
40	150480.0
41	166539.0
42	185080.5
43	203622.0
44	209351.0
45	215080.0
46	221939.5
47	228799.0
48	229207.0
49	229615.0
50	220948.5
51	212282.0
52	204542.5
53	196803.0
54	180466.0
55	164129.0
56	152200.5
57	140272.0
58	132216.5
59	124161.0
60	114731.0
61	105301.0
62	92128.5
63	78956.0
64	69254.5
65	59553.0
66	48603.0
67	37653.0
68	32714.5
69	27776.0
70	23826.5
71	19877.0
72	16160.5
73	12444.0
74	10592.5
75	6799.0
76	4857.0
77	3804.5
78	2752.0
79	2163.0
80	1574.0
81	1144.5
82	715.0
83	578.5
84	442.0
85	306.5
86	171.0
87	147.5
88	124.0
89	78.0
90	32.0
91	21.5
92	11.0
93	7.5
94	4.0
95	4.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3180488.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.659360022483867
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.67756664545082	18.378015149352052
2	6.505470067843018	3.07830516901181
3	2.8520437999698744	2.024325931901407
4	1.8005164580989477	1.7039626843428195
5	1.299320225116739	1.5370542495265853
6	0.9853946861167874	1.3988284585847732
7	0.7419694327392751	1.2288165354399645
8	0.6195620654589101	1.1726753570372859
9	0.5199244151614331	1.1070971030505343
>10	5.377089234394203	27.62899243851114
>50	1.0077478469316192	16.882894152945184
>100	0.608428373126021	21.593437719422283
>500	0.003490148362181381	0.5783720908302604
>1k	0.001342364754685146	0.3989627261001443
>5k	0.0	0.0
>10k+	1.342364754685146E-4	1.288260233943652
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	40563	1.2753703205294282	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01685275970228468	0.0
2	0.0	0.0	0.0	0.06011656072904535	0.0
3	0.0	0.0	0.0	0.09957591413644698	0.0
4	0.0	0.0	0.0	0.13884661724867378	0.0
5	0.0	0.0	0.0	0.23307743968850064	0.0
6	0.0	0.0	0.0	0.4161625511556717	0.0
7	0.0	0.0	0.0	0.5496326349918629	0.0
8	0.0	0.0	0.0	0.8189938147856556	0.0
9	3.144171586247142E-5	0.0	0.0	0.9867353689119406	0.0
10	3.144171586247142E-5	0.0	0.0	1.2060413370526788	0.0
11	3.144171586247142E-5	0.0	0.0	1.3554838125470055	0.0
12	3.144171586247142E-5	0.0	0.0	1.4890167798149214	0.0
13	6.288343172494284E-5	0.0	0.0	1.5553273585688736	0.0
14	6.288343172494284E-5	0.0	0.0	1.592365699854865	0.0
15	6.288343172494284E-5	0.0	0.0	1.6215436121752385	0.0
16	6.288343172494284E-5	0.0	0.0	1.6725106335883047	0.0
17	6.288343172494284E-5	0.0	0.0	1.733224586918737	0.0
18	6.288343172494284E-5	0.0	0.0	1.829530562605487	0.0
19	9.432514758741426E-5	0.0	0.0	1.8721969710308606	0.0
20	1.2576686344988568E-4	0.0	0.0	1.92231506611564	0.0
21	1.2576686344988568E-4	0.0	0.0	1.9779669031922145	0.0
22	1.2576686344988568E-4	0.0	0.0	2.038020580489535	0.0
23	1.2576686344988568E-4	0.0	0.0	2.1063434290586853	0.0
24	1.2576686344988568E-4	0.0	0.0	2.162938517611134	0.0
25	1.2576686344988568E-4	0.0	0.0	2.215163207658699	0.0
26	1.572085793123571E-4	0.0	0.0	2.268016732023513	0.0
27	1.572085793123571E-4	0.0	0.0	2.3272529247084095	0.0
28	1.572085793123571E-4	0.0	0.0	2.3853257739063944	0.0
29	1.572085793123571E-4	0.0	0.0	2.4542460150769316	0.0
30	1.572085793123571E-4	0.0	0.0	2.5516838925347307	0.0
31	1.572085793123571E-4	0.0	0.0	2.622616403520466	0.0
32	1.886502951748285E-4	0.0	0.0	2.6923541293034274	0.0
33	1.886502951748285E-4	0.0	0.0	2.7641670083333123	0.0
34	2.2009201103729995E-4	0.0	0.0	2.8436831077495026	0.0
35	2.2009201103729995E-4	0.0	0.0	2.933543531684446	0.0
36	2.2009201103729995E-4	0.0	0.0	3.0110159195695756	0.0
37	2.2009201103729995E-4	0.0	0.0	3.095531251807899	0.0
38	2.5153372689977135E-4	0.0	0.0	3.1936922887305346	0.0
39	2.5153372689977135E-4	0.0	0.0	3.3455557763462713	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	34805	0.0	43.978596	1
TATCGCG	95	0.0	42.63158	1
CGACGGT	880	0.0	41.676132	28
CGGTCTA	875	0.0	41.657143	31
GTCGGTA	55	6.184564E-11	40.90909	11
TCTAGCG	125	0.0	39.600002	1
GTTTTTT	39540	0.0	39.429058	2
TCACGAC	945	0.0	39.285717	25
AGGGCGA	2750	0.0	39.27273	6
ACGGGAT	745	0.0	39.261745	5
TAGGGCG	1330	0.0	39.24812	5
GCGTAAG	195	0.0	39.23077	1
GCGATAC	155	0.0	39.193546	9
GGCGATA	1165	0.0	38.819744	8
ACGGTCT	940	0.0	38.537235	30
ACGACGG	965	0.0	38.238342	27
GGGCGAT	4950	0.0	38.136364	7
GTAGGGT	1615	0.0	37.894737	4
TAGGGTA	1645	0.0	37.88754	5
TACGTAG	155	0.0	37.741936	1
>>END_MODULE