FastQCFastQC Report
Thu 26 May 2016
SRR1545160_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545160_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1612368
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118080.7323390193801911No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGTA351.2123382E-745.00000430
TCGAATA207.034134E-445.031
CCCGTAT302.166049E-644.99999633
CGGTCTA3000.043.531
CGTTTTT91250.043.21
TACGGGA6350.041.456694
GTATGCG1100.040.9090921
CGACGGT3200.040.7812528
ACGGTCT3200.040.07812530
GCGTTAG1700.039.7058831
CGGGATA4250.039.7058836
AGGGTAC4150.039.5783126
ATGACGG1600.039.3752
CGTACAT403.4593722E-739.37535
TCGATTG356.249855E-638.571431
GCGTAAG1400.038.571431
TAGGGTA8000.038.531255
CGTTAGG2950.038.1355932
ATAGGGC7200.038.1254
ACGGGAT6350.037.9133875
Produced by FastQC (version 0.11.5)