FastQCFastQC Report
Thu 26 May 2016
SRR1545159_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545159_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1683131
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89170.5297864515596231No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC23270.1382542416484516No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19200.11407311730340657No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGC302.1661053E-645.00000441
TGCGCAT302.1661053E-645.0000041
ACGCTTA302.1661053E-645.00000444
CGCAGAT302.1661053E-645.00000423
TGTCGAT302.1661053E-645.00000425
CTATGCG502.1827873E-1145.01
AATCCCG207.0342224E-445.036
ACACGTA253.89158E-545.024
AGTTTCG207.0342224E-445.032
ATTCGGT453.8562575E-1045.029
TTCGTAA207.0342224E-445.030
TAGCCGT406.8175723E-945.044
CCGTATG253.89158E-545.024
GCGATCG351.2123746E-745.09
CTACCGT207.0342224E-445.021
TCCGTCG253.89158E-545.027
CGTACAA207.0342224E-445.029
ACCCGTT207.0342224E-445.039
GACGATA502.1827873E-1145.09
CCCGGTA406.8175723E-945.042