##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545159_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1683131
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.02753142803501	31.0	31.0	33.0	28.0	34.0
2	31.315069949992008	31.0	31.0	34.0	28.0	34.0
3	31.382890577144618	31.0	31.0	34.0	28.0	34.0
4	35.16683252818705	35.0	35.0	37.0	32.0	37.0
5	35.01244525827164	35.0	35.0	37.0	32.0	37.0
6	35.06970105119566	37.0	35.0	37.0	32.0	37.0
7	35.72035866489299	37.0	35.0	37.0	35.0	37.0
8	35.744514241612805	37.0	35.0	37.0	35.0	37.0
9	37.460056882084636	39.0	37.0	39.0	35.0	39.0
10	36.58927023505598	39.0	35.0	39.0	32.0	39.0
11	36.207248871299974	38.0	35.0	39.0	31.0	39.0
12	35.379369757909515	37.0	35.0	39.0	30.0	39.0
13	35.00553373445086	37.0	34.0	39.0	29.0	39.0
14	35.93875402449364	38.0	34.0	40.0	29.0	41.0
15	36.31318001985585	38.0	35.0	40.0	30.0	41.0
16	36.46691434000087	38.0	35.0	40.0	31.0	41.0
17	36.37959790414412	38.0	35.0	40.0	31.0	41.0
18	36.25219249125588	38.0	35.0	40.0	31.0	41.0
19	36.1419550825218	37.0	35.0	40.0	30.0	41.0
20	35.96086341467182	37.0	34.0	40.0	30.0	41.0
21	35.82021244929836	37.0	34.0	40.0	30.0	41.0
22	35.733284574997434	36.0	34.0	40.0	30.0	41.0
23	35.70071967066141	36.0	34.0	40.0	30.0	41.0
24	35.59830161763998	36.0	34.0	40.0	30.0	41.0
25	35.52038433134438	36.0	34.0	40.0	29.0	41.0
26	35.3488658933856	36.0	34.0	40.0	29.0	41.0
27	35.25610662509335	36.0	34.0	40.0	29.0	41.0
28	35.247879695638666	36.0	34.0	40.0	29.0	41.0
29	35.25570677505197	36.0	34.0	40.0	29.0	41.0
30	35.16682718100968	36.0	34.0	40.0	29.0	41.0
31	34.94704630833845	36.0	34.0	40.0	27.0	41.0
32	34.68655024475219	36.0	33.0	40.0	26.0	41.0
33	34.390140161401575	36.0	33.0	40.0	24.0	41.0
34	34.19360822181993	35.0	33.0	40.0	24.0	41.0
35	33.95922896078796	35.0	33.0	40.0	23.0	41.0
36	33.7289373197927	35.0	33.0	40.0	22.0	41.0
37	33.66457750466244	35.0	33.0	40.0	22.0	41.0
38	33.65531916410547	35.0	33.0	40.0	23.0	41.0
39	33.60873336656505	35.0	33.0	40.0	22.0	41.0
40	33.449013178415704	35.0	32.0	40.0	21.0	41.0
41	33.42378697795953	35.0	32.0	40.0	21.0	41.0
42	33.34304281722575	35.0	32.0	40.0	21.0	41.0
43	33.27427276902392	35.0	32.0	40.0	21.0	41.0
44	33.1615299106249	35.0	32.0	39.0	20.0	41.0
45	33.078998604386705	35.0	32.0	39.0	20.0	41.0
46	32.97450287589023	35.0	32.0	39.0	20.0	41.0
47	32.861620990879494	35.0	32.0	39.0	20.0	41.0
48	32.827309341934765	35.0	32.0	39.0	20.0	41.0
49	32.83824372553295	35.0	32.0	39.0	20.0	41.0
50	32.75658163268337	35.0	31.0	39.0	19.0	41.0
51	32.608155871408705	35.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	2.0
13	4.0
14	20.0
15	76.0
16	266.0
17	693.0
18	1511.0
19	2964.0
20	5070.0
21	7796.0
22	11411.0
23	15641.0
24	22223.0
25	30232.0
26	36998.0
27	41015.0
28	43331.0
29	48097.0
30	56445.0
31	67550.0
32	82382.0
33	103312.0
34	163790.0
35	227866.0
36	120948.0
37	143022.0
38	187127.0
39	263175.0
40	162.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.720009315971247	22.996486904465545	30.49780438955732	15.785699390005888
2	31.978616043552165	25.461654499857705	30.125819083600742	12.433910372989388
3	27.222480008983258	25.543822792165315	34.19062449684546	13.043072702005965
4	24.778285231511983	27.027248621765033	33.71852814783876	14.475937998884222
5	21.56451280381622	32.756689764492485	32.47091284041468	13.207884591276613
6	19.14788569635994	40.436424734616615	29.798690654500454	10.616998914522993
7	85.15486911000986	4.6720665236395735	8.531421499574305	1.6416428667762641
8	87.0307777588316	3.135050094140028	8.127293716294217	1.7068784307341496
9	83.80904397815738	4.623704274949484	9.32108077149075	2.2461709754023897
10	48.08853262164383	25.14349744612867	15.727949874371038	11.04002005785646
11	41.71671723710157	21.473194896891567	22.575604632081518	14.234483233925346
12	37.154327262702665	20.66903883298448	27.288666182252	14.88796772206085
13	20.150897345482914	39.16040997403054	26.96076538308664	13.727927297399905
14	14.732246034325314	40.213447438137614	29.874917638615177	15.179388888921896
15	12.441218182066637	22.19328144986932	48.608991219340616	16.75650914872342
16	15.707392948023655	19.10207821019279	45.84610467040296	19.3444241713806
17	16.21175060051773	20.25801913220064	30.083160490775825	33.44706977650581
18	20.28107140798904	23.6449212806371	36.6436124104422	19.43039490093166
19	27.296508709066615	25.56758802493686	27.525308487574645	19.61059477842188
20	29.1621983077966	22.693658425874162	27.996038335696984	20.148104930632257
21	20.235858052641177	28.315858955720024	30.147742510832494	21.30054048080631
22	20.898551568475657	25.339144724920402	26.934088909300584	26.828214797303357
23	17.974358502101143	30.815248486303204	26.309003874327075	24.901389137268577
24	17.877574591639032	24.779295253904777	39.707723284759176	17.63540686969701
25	16.504894746754708	27.152491398471064	35.525101730049535	20.817512124724693
26	17.338400873134653	34.00377035417921	28.151641197268663	20.50618757541748
27	17.056782864791867	34.25586006080335	28.830079179814287	19.857277894590496
28	15.56509861680404	28.205469449496206	37.37724514609974	18.852186787600015
29	15.708521796580301	23.798801162832838	37.60325250975711	22.889424530829743
30	17.914826594008428	29.861371455935398	32.923284046221	19.300517903835175
31	26.978351655337583	28.131916054068277	25.957575494717883	18.932156795876256
32	28.69348850445984	25.804586808751072	26.821322879799613	18.680601806989475
33	27.53392338445433	27.78173534917959	25.59569041268921	19.088650853676867
34	20.20621092475868	28.009346866049047	27.276248848128876	24.508193361063398
35	19.29142770230006	27.69606168503818	31.66735090732688	21.34515970533488
36	29.41339681819181	26.667027106030368	26.02887119303251	17.89070488274531
37	20.62691495789692	33.79932993926201	27.653521918377123	17.920233184463953
38	19.67933571421357	32.89048802499627	25.94925766324784	21.480918597542317
39	19.817292890452375	34.18723795117552	26.200753239052695	19.79471591931941
40	23.85453063368211	27.83496947058785	25.67874990122575	22.63174999450429
41	18.14237869779595	26.41410561625922	26.873725218060862	28.56979046788396
42	20.33192900612014	27.54533069618467	24.58204382190097	27.540696475794217
43	21.71898681683125	26.95773531590827	26.052517599640197	25.270760267620286
44	19.979015299462727	29.028162394965097	28.992811611217427	22.000010694354746
45	17.53707821910475	37.23471316255241	23.579566890515355	21.648641727827485
46	21.829673388464713	32.97503284058104	26.636667021164723	18.55862674978953
47	21.4913752999618	29.017230387890187	26.229330931460478	23.26206338068754
48	22.230295799910998	24.72915061275682	30.283085511466428	22.757468075865752
49	20.490977826443693	25.067448701259735	31.938749865577904	22.50282360671867
50	18.80293334268099	32.41583691346663	28.102625404677354	20.678604339175024
51	18.094372927597433	32.627109832805644	25.911946247796518	23.3665709918004
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2258.0
1	2217.0
2	2176.0
3	8731.5
4	15287.0
5	12053.5
6	8820.0
7	9565.5
8	10311.0
9	11021.0
10	11731.0
11	12115.5
12	12500.0
13	12479.5
14	12459.0
15	12174.0
16	11889.0
17	11426.5
18	10964.0
19	10533.5
20	10103.0
21	9866.0
22	9629.0
23	9807.5
24	9986.0
25	10376.0
26	13950.0
27	17134.0
28	19240.0
29	21346.0
30	23155.5
31	24965.0
32	30583.0
33	36201.0
34	40696.0
35	45191.0
36	50507.5
37	55824.0
38	66674.0
39	77524.0
40	97090.5
41	116657.0
42	136151.5
43	155646.0
44	158684.5
45	161723.0
46	155855.0
47	149987.0
48	143619.0
49	137251.0
50	127746.0
51	118241.0
52	108097.0
53	97953.0
54	87109.5
55	76266.0
56	68665.5
57	61065.0
58	55680.0
59	50295.0
60	44723.0
61	39151.0
62	35285.5
63	31420.0
64	27287.5
65	23155.0
66	18604.5
67	14054.0
68	12478.5
69	10903.0
70	9634.5
71	8366.0
72	7134.0
73	5902.0
74	4820.0
75	2675.0
76	1612.0
77	1495.0
78	1378.0
79	1084.5
80	791.0
81	553.5
82	316.0
83	227.0
84	138.0
85	92.0
86	46.0
87	27.0
88	8.0
89	5.5
90	3.0
91	2.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1683131.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.789192065503638
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.25455125296399	17.48681831381638
2	7.3918497055871395	3.2212486590874936
3	2.9415174466816305	1.922798658293277
4	1.5990753665258175	1.3937024115378667
5	0.9864096108925703	1.0746534233498461
6	0.678976999823533	0.8876616154328635
7	0.5089997411786498	0.776348518528327
8	0.4120115372119229	0.7181918814011191
9	0.32047234393465085	0.6284550108306822
>10	3.120843040510569	14.671917357157726
>50	0.7118203825535498	11.123553336451268
>100	1.064444897599844	44.22839025729463
>500	0.008206976851066786	1.0815835000629956
>1k	5.471317900711192E-4	0.25315431935891664
>5k	2.735658950355596E-4	0.5315227373966235
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8917	0.5297864515596231	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2327	0.1382542416484516	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1920	0.11407311730340657	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.020853991757029015	0.0
2	0.0	0.0	0.0	0.10064576078748476	0.0
3	0.0	0.0	0.0	0.16813902185866697	0.0
4	0.0	0.0	0.0	0.2579121886531708	0.0
5	0.0	0.0	0.0	0.4729281321537064	0.0
6	0.0	0.0	0.0	0.7855003561814262	0.0
7	0.0	0.0	0.0	0.994159099915574	0.0
8	0.0	0.0	0.0	1.5197272226582483	0.0
9	5.941308192885759E-5	0.0	0.0	1.8024146664757525	0.0
10	5.941308192885759E-5	0.0	0.0	2.225435809809219	0.0
11	5.941308192885759E-5	0.0	0.0	2.551078911861287	0.0
12	5.941308192885759E-5	0.0	0.0	2.82033899916287	0.0
13	5.941308192885759E-5	0.0	0.0	2.9500377570135656	0.0
14	5.941308192885759E-5	0.0	0.0	3.000835942062739	0.0
15	5.941308192885759E-5	0.0	0.0	3.0590607623530195	0.0
16	5.941308192885759E-5	0.0	0.0	3.1850759091241265	0.0
17	5.941308192885759E-5	0.0	0.0	3.3249343039846573	0.0
18	5.941308192885759E-5	0.0	0.0	3.5319888945067257	0.0
19	5.941308192885759E-5	0.0	0.0	3.62693099942904	0.0
20	5.941308192885759E-5	0.0	0.0	3.7424300306987393	0.0
21	5.941308192885759E-5	0.0	0.0	3.871118766156645	0.0
22	5.941308192885759E-5	0.0	0.0	4.0005798716796255	0.0
23	5.941308192885759E-5	0.0	0.0	4.160579301314039	0.0
24	5.941308192885759E-5	0.0	0.0	4.273285917733082	0.0
25	5.941308192885759E-5	0.0	0.0	4.365079129313167	0.0
26	5.941308192885759E-5	0.0	0.0	4.464417802298217	0.0
27	5.941308192885759E-5	0.0	0.0	4.564766497676057	0.0
28	5.941308192885759E-5	0.0	0.0	4.677235461767385	0.0
29	5.941308192885759E-5	0.0	0.0	4.794041580839519	0.0
30	1.1882616385771517E-4	0.0	0.0	4.956179881423371	0.0
31	1.1882616385771517E-4	0.0	0.0	5.080769114228185	0.0
32	1.1882616385771517E-4	0.0	0.0	5.197634646382248	0.0
33	1.1882616385771517E-4	0.0	0.0	5.322283292268992	0.0
34	1.7823924578657277E-4	0.0	0.0	5.441525347700209	0.0
35	1.7823924578657277E-4	0.0	0.0	5.604257779103349	0.0
36	1.7823924578657277E-4	0.0	0.0	5.733659471544401	0.0
37	1.7823924578657277E-4	0.0	0.0	5.864427664869817	0.0
38	1.7823924578657277E-4	0.0	0.0	5.999295360848324	0.0
39	1.7823924578657277E-4	0.0	0.0	6.142480888296871	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTACGC	30	2.1661053E-6	45.000004	41
TGCGCAT	30	2.1661053E-6	45.000004	1
ACGCTTA	30	2.1661053E-6	45.000004	44
CGCAGAT	30	2.1661053E-6	45.000004	23
TGTCGAT	30	2.1661053E-6	45.000004	25
CTATGCG	50	2.1827873E-11	45.0	1
AATCCCG	20	7.0342224E-4	45.0	36
ACACGTA	25	3.89158E-5	45.0	24
AGTTTCG	20	7.0342224E-4	45.0	32
ATTCGGT	45	3.8562575E-10	45.0	29
TTCGTAA	20	7.0342224E-4	45.0	30
TAGCCGT	40	6.8175723E-9	45.0	44
CCGTATG	25	3.89158E-5	45.0	24
GCGATCG	35	1.2123746E-7	45.0	9
CTACCGT	20	7.0342224E-4	45.0	21
TCCGTCG	25	3.89158E-5	45.0	27
CGTACAA	20	7.0342224E-4	45.0	29
ACCCGTT	20	7.0342224E-4	45.0	39
GACGATA	50	2.1827873E-11	45.0	9
CCCGGTA	40	6.8175723E-9	45.0	42
>>END_MODULE