FastQCFastQC Report
Thu 26 May 2016
SRR1545158_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545158_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1855876
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160730.866060016940787No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCG406.8193913E-945.01
CGTTTTT131100.043.678491
CGACGGT4250.042.3529428
TCACGAC4450.041.46067425
ACGGTCT4400.041.42045630
ACGTTAG603.6379788E-1241.2499961
CACGACG4500.041.026
CGGTCTA4450.040.95505531
TCGGTTA501.0822987E-940.542
ACGGGTA3450.040.4347845
CGAGGGA10100.040.3217854
CGGGTAT2850.040.2631576
CGTAAGG3850.039.740262
TATGACG1700.039.7058831
TAGCACG750.039.01
TATGGGC9550.038.6387444
AATCCCG356.250364E-638.5714313
ATACGGT356.250364E-638.5714322
GTTTTTT154000.038.3522722
TTACGGG5050.038.3168333
Produced by FastQC (version 0.11.5)