##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545158_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1855876
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.081384208858783	31.0	31.0	33.0	30.0	34.0
2	31.383471740568876	31.0	31.0	34.0	30.0	34.0
3	31.448403341602564	31.0	31.0	34.0	30.0	34.0
4	35.2585054173878	37.0	35.0	37.0	33.0	37.0
5	35.14187478042714	37.0	35.0	37.0	32.0	37.0
6	35.19826971198507	37.0	35.0	37.0	32.0	37.0
7	35.772535988395774	37.0	35.0	37.0	35.0	37.0
8	35.77376128577556	37.0	35.0	37.0	35.0	37.0
9	37.496385534378376	39.0	37.0	39.0	35.0	39.0
10	36.63901036491662	39.0	35.0	39.0	32.0	39.0
11	36.35816132112275	38.0	35.0	39.0	32.0	39.0
12	35.86479215206188	37.0	35.0	39.0	31.0	39.0
13	35.672735139632174	37.0	35.0	39.0	30.0	39.0
14	36.61538648056228	38.0	35.0	41.0	31.0	41.0
15	36.82984854591578	38.0	35.0	41.0	31.0	41.0
16	36.91222527798193	38.0	35.0	41.0	31.0	41.0
17	36.838290920298554	38.0	35.0	41.0	31.0	41.0
18	36.693600218980144	38.0	35.0	40.0	31.0	41.0
19	36.59532102360287	38.0	35.0	40.0	31.0	41.0
20	36.41685651412055	38.0	35.0	40.0	31.0	41.0
21	36.30612336169011	38.0	35.0	40.0	30.0	41.0
22	36.2436827675987	38.0	35.0	40.0	30.0	41.0
23	36.157186687041595	38.0	34.0	40.0	30.0	41.0
24	36.05170873485082	38.0	34.0	40.0	30.0	41.0
25	35.94436266216062	37.0	34.0	40.0	30.0	41.0
26	35.82005532697227	37.0	34.0	40.0	30.0	41.0
27	35.73367024521035	37.0	34.0	40.0	30.0	41.0
28	35.696651069360236	37.0	34.0	40.0	29.0	41.0
29	35.61547969799707	37.0	34.0	40.0	29.0	41.0
30	35.48997077390946	37.0	34.0	40.0	29.0	41.0
31	35.296811317135415	37.0	34.0	40.0	27.0	41.0
32	35.086864639663425	37.0	34.0	40.0	26.0	41.0
33	34.76864725876082	37.0	33.0	40.0	25.0	41.0
34	34.46725266127694	37.0	33.0	40.0	23.0	41.0
35	34.2515927788279	37.0	33.0	40.0	23.0	41.0
36	34.05944147130519	37.0	33.0	40.0	22.0	41.0
37	33.980322499994614	37.0	33.0	40.0	22.0	41.0
38	33.913375139287325	37.0	33.0	40.0	22.0	41.0
39	33.83231476671933	36.0	33.0	40.0	21.0	41.0
40	33.69962594483683	36.0	33.0	40.0	21.0	41.0
41	33.59989945448942	36.0	33.0	40.0	20.0	41.0
42	33.52631425806466	36.0	32.0	40.0	20.0	41.0
43	33.47226862139497	36.0	32.0	40.0	20.0	41.0
44	33.33825643523598	35.0	32.0	40.0	20.0	41.0
45	33.28063620629827	35.0	32.0	40.0	20.0	41.0
46	33.219160655129976	35.0	32.0	40.0	20.0	41.0
47	33.11097670318491	35.0	32.0	40.0	20.0	41.0
48	33.026854164825664	35.0	32.0	39.0	20.0	41.0
49	32.95836359756794	35.0	31.0	39.0	19.0	41.0
50	32.9057674111848	35.0	31.0	39.0	19.0	41.0
51	32.74750576008311	35.0	31.0	39.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	2.0
12	3.0
13	5.0
14	19.0
15	74.0
16	260.0
17	662.0
18	1507.0
19	3009.0
20	5044.0
21	7935.0
22	11448.0
23	16714.0
24	23265.0
25	32010.0
26	40675.0
27	44856.0
28	46531.0
29	49865.0
30	58042.0
31	68487.0
32	83832.0
33	104688.0
34	155259.0
35	198641.0
36	151330.0
37	183797.0
38	240435.0
39	327322.0
40	156.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.36159096836211	22.91731775183256	27.50022091993215	17.22087035987318
2	34.79505096245654	24.1261808439788	28.42689921093866	12.651868982625993
3	28.050580965538646	24.656604212781456	34.73567199532727	12.55714282635262
4	24.691628104463874	26.685996262681343	34.165105858365536	14.457269774489243
5	22.088490825895697	30.93094581750074	33.19273485944104	13.787828497162527
6	19.522856052882844	40.23528511603146	30.56265612573254	9.679202705353159
7	85.08655750707483	3.5693117428104033	9.848287277813819	1.495843472300951
8	86.47689824104627	2.653248385129179	9.408441081192924	1.4614122926316198
9	82.44354687489897	4.299101879651443	10.92470617649024	2.3326450689593483
10	41.23691453523834	26.432746584362317	19.178975319471775	13.151363560927562
11	34.75862611510683	23.308669329200875	26.19436858928075	15.73833596641155
12	32.05499720886525	21.219736663440877	29.66135668546821	17.063909442225665
13	23.138776513086004	28.953766307662796	30.030185206339215	17.877271972911984
14	19.317885462175273	30.513245497005187	31.84851789666982	18.320351144149715
15	18.26706094588216	23.840439770760547	39.42224588280683	18.47025340055047
16	20.911580299545875	20.330022048886885	37.88749894928325	20.870898702283988
17	20.851446971672676	21.875976627748837	31.01807448342454	26.25450191715395
18	22.626781099599327	22.98860484213385	33.98298162161696	20.401632436649862
19	26.53339986076656	25.011369294069212	27.82588922966836	20.629341615495864
20	27.52991040349679	24.264606040489774	28.880000603488593	19.325482952524844
21	24.155978093364	26.085525110513846	30.78783280779535	18.970663988326805
22	23.19271330627693	23.102674963197973	29.672133267524337	24.03247846300076
23	20.849022240710045	26.750763520838678	29.80274544204462	22.597468796406655
24	20.544368265983287	24.91470335302574	35.93025611624915	18.610672264741826
25	21.064877179294307	25.396470453844977	32.89045173276663	20.64820063409409
26	19.90375434565671	28.416930872536746	29.645030163653175	22.034284618153368
27	19.460190228226455	28.405346046826402	30.97205847804487	21.16240524690227
28	19.234151419599154	26.08002905366522	34.088915423228705	20.596904103506915
29	19.702932739040754	23.555776355747906	34.3826311671685	22.358659738042842
30	21.5899122570689	26.433231530554842	31.862042507150264	20.114813705225995
31	25.01681146800756	25.58743148787958	29.3173143033263	20.078442740786564
32	26.560179667176037	24.855162737165628	28.359599455998136	20.22505813966019
33	26.33845149137119	25.794719043729213	27.445152585625333	20.421676879274262
34	20.928176235912314	26.651834497563414	29.39937797568372	23.020611290840552
35	21.59648597212314	25.984656302468483	30.069519730844085	22.34933799456429
36	25.99117613461244	27.035265287120474	26.06203216163149	20.911526416635596
37	21.621110462121393	30.26910203052359	27.55135580178848	20.558431705566534
38	22.577801534154222	29.408322538790305	26.728132698520806	21.285743228534663
39	21.391784795966974	29.166819334912464	26.49401145335141	22.947384415769157
40	24.388321202494133	26.174054732104945	27.183712704943648	22.25391136045727
41	21.00662975328093	24.739853309165053	28.55584101524024	25.697675922313774
42	21.2686623459757	26.78196172589117	26.557701053303127	25.39167487483
43	21.902055956324666	25.835508406811662	27.88510654806679	24.37732908879688
44	21.85932680847212	27.026644021475573	28.548997885634602	22.565031284417707
45	20.148059460869153	30.693807129355626	25.777476512439407	23.380656897335815
46	21.83524114757667	28.83118268677433	27.59553978821861	21.73803637743039
47	21.508225765083445	27.731701902497797	27.612459022046732	23.14761331037203
48	21.4793984080833	26.956434589379896	29.568516431054658	21.995650571482148
49	21.800540553355933	25.849625729305192	29.86023850731407	22.489595210024806
50	20.788565615375166	28.977043724904032	28.2054943325955	22.028896327125302
51	20.0683127536538	29.524601859175938	27.332214005677102	23.07487138149316
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1493.0
1	1990.5
2	2488.0
3	14659.5
4	26831.0
5	19580.5
6	12330.0
7	12682.0
8	13034.0
9	13692.0
10	14350.0
11	14631.5
12	14913.0
13	14619.0
14	14325.0
15	13827.5
16	13330.0
17	12544.5
18	11759.0
19	11450.5
20	11142.0
21	11081.0
22	11020.0
23	11228.0
24	11436.0
25	11311.5
26	14624.5
27	18062.0
28	21786.0
29	25510.0
30	27068.0
31	28626.0
32	33138.5
33	37651.0
34	46961.0
35	56271.0
36	57881.0
37	59491.0
38	66765.0
39	74039.0
40	86393.0
41	98747.0
42	110418.0
43	122089.0
44	123657.5
45	125226.0
46	126235.0
47	127244.0
48	126789.0
49	126334.0
50	123784.0
51	121234.0
52	115800.0
53	110366.0
54	105483.0
55	100600.0
56	96398.5
57	92197.0
58	88486.5
59	84776.0
60	78378.0
61	71980.0
62	65825.5
63	59671.0
64	53005.5
65	46340.0
66	38864.0
67	31388.0
68	27313.0
69	23238.0
70	19730.0
71	16222.0
72	14336.5
73	12451.0
74	10122.0
75	5953.5
76	4114.0
77	3101.0
78	2088.0
79	1772.5
80	1457.0
81	1038.5
82	620.0
83	412.5
84	205.0
85	180.0
86	155.0
87	97.5
88	40.0
89	24.5
90	9.0
91	5.5
92	2.0
93	1.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1855876.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.94046973409495
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.47787843411975	16.99901046845692
2	6.739607767119404	2.9574032046830907
3	2.911344414247088	1.9162879201894387
4	1.7353504435457838	1.5229761553865808
5	1.151463469912682	1.2631824705767576
6	0.9111309104884063	1.1994384099221551
7	0.7206393161945072	1.1067815574315127
8	0.59172171192381	1.0386121849176966
9	0.4834126653657144	0.9545670858181166
>10	5.304937163435778	25.860646082845683
>50	1.2050252742007508	18.969594164794433
>100	0.7642843994301454	24.81949325707653
>500	0.0024646384740310786	0.3853948472970644
>1k	4.929276948062157E-4	0.13745947829790064
>5k	0.0	0.0
>10k+	2.4646384740310785E-4	0.8691527123061199
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16073	0.866060016940787	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.014009556672967376	0.0
2	0.0	0.0	0.0	0.04509999590489882	0.0
3	0.0	0.0	0.0	0.07403511872560452	0.0
4	0.0	0.0	0.0	0.11563272546226148	0.0
5	0.0	0.0	0.0	0.2012526698982044	0.0
6	0.0	0.0	0.0	0.3725464416803709	0.0
7	0.0	0.0	0.0	0.5012188314305481	0.0
8	0.0	0.0	0.0	0.8124465212115465	0.0
9	0.0	0.0	0.0	0.9918766124460902	0.0
10	0.0	0.0	0.0	1.2321943922977612	0.0
11	0.0	0.0	0.0	1.3922266358312732	0.0
12	0.0	0.0	0.0	1.5254251900450246	0.0
13	0.0	0.0	0.0	1.5930482424472325	0.0
14	0.0	0.0	0.0	1.626240115180109	0.0
15	0.0	0.0	0.0	1.6540975797952018	0.0
16	0.0	0.0	0.0	1.707010597690794	0.0
17	0.0	0.0	0.0	1.76612015026866	0.0
18	0.0	0.0	0.0	1.8578288635663158	0.0
19	0.0	0.0	0.0	1.9021744987272857	0.0
20	0.0	0.0	0.0	1.9526627856602488	0.0
21	0.0	0.0	0.0	2.0124728160717633	0.0
22	0.0	0.0	0.0	2.077725020421623	0.0
23	0.0	0.0	0.0	2.150628598031334	0.0
24	0.0	0.0	0.0	2.2084988436727455	0.0
25	0.0	0.0	0.0	2.2603880862730055	0.0
26	0.0	0.0	0.0	2.3102836611928814	0.0
27	0.0	0.0	0.0	2.3718179447333765	0.0
28	0.0	0.0	0.0	2.4320590384271363	0.0
29	5.388291028064375E-5	0.0	0.0	2.502160704702254	0.0
30	5.388291028064375E-5	0.0	0.0	2.6023829178242512	0.0
31	5.388291028064375E-5	0.0	0.0	2.679112182063888	0.0
32	5.388291028064375E-5	0.0	0.0	2.7458731079016054	0.0
33	5.388291028064375E-5	0.0	0.0	2.8198004608066487	0.0
34	1.077658205612875E-4	0.0	0.0	2.8995471680220013	0.0
35	1.077658205612875E-4	0.0	0.0	2.9981528938355795	0.0
36	1.077658205612875E-4	0.0	0.0	3.081725287680858	0.0
37	1.077658205612875E-4	0.0	0.0	3.1738650642607587	0.0
38	1.077658205612875E-4	0.0	0.0	3.2891744922613366	0.0
39	1.077658205612875E-4	0.0	0.0	3.5026046998829665	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	40	6.8193913E-9	45.0	1
CGTTTTT	13110	0.0	43.67849	1
CGACGGT	425	0.0	42.35294	28
TCACGAC	445	0.0	41.460674	25
ACGGTCT	440	0.0	41.420456	30
ACGTTAG	60	3.6379788E-12	41.249996	1
CACGACG	450	0.0	41.0	26
CGGTCTA	445	0.0	40.955055	31
TCGGTTA	50	1.0822987E-9	40.5	42
ACGGGTA	345	0.0	40.434784	5
CGAGGGA	1010	0.0	40.321785	4
CGGGTAT	285	0.0	40.263157	6
CGTAAGG	385	0.0	39.74026	2
TATGACG	170	0.0	39.705883	1
TAGCACG	75	0.0	39.0	1
TATGGGC	955	0.0	38.638744	4
AATCCCG	35	6.250364E-6	38.57143	13
ATACGGT	35	6.250364E-6	38.57143	22
GTTTTTT	15400	0.0	38.352272	2
TTACGGG	505	0.0	38.316833	3
>>END_MODULE