FastQCFastQC Report
Thu 26 May 2016
SRR1545157_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545157_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2377941
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72880.30648363437107984No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29250.12300557499113729No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC24530.10315647024043068No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGTA207.034809E-445.00000445
CGGCGGA253.8920676E-545.0000044
CGTGCTA207.034809E-445.00000428
CCGGATA207.034809E-445.00000420
CCAATCG502.1827873E-1145.00000440
GCCGGAT207.034809E-445.00000419
GCGCCGA207.034809E-445.00000432
CCGTCCC207.034809E-445.00000411
ATATCCG850.045.0000041
TCGTGAC406.8212103E-945.00000422
CTACGCT406.8212103E-945.00000421
CGGGCTA1650.045.0000046
AACGACG406.8212103E-945.00000443
CTCGCTA406.8212103E-945.00000427
ACGCTAC406.8212103E-945.00000423
ACCGTAG900.045.0000041
TCGAGCC207.034809E-445.00000416
CCAACGC207.034809E-445.00000413
ATTGCGA207.034809E-445.0000041
TCGGTTA207.034809E-445.00000436