##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545157_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2377941
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.83371917133352	31.0	31.0	33.0	28.0	33.0
2	31.140058983801534	31.0	31.0	33.0	30.0	34.0
3	31.314684006037155	31.0	31.0	34.0	28.0	34.0
4	35.14521848944108	35.0	35.0	37.0	32.0	37.0
5	35.017931479376486	35.0	35.0	37.0	32.0	37.0
6	35.094023779395705	37.0	35.0	37.0	32.0	37.0
7	35.566149033975194	36.0	35.0	37.0	35.0	37.0
8	35.648398761785934	37.0	35.0	37.0	35.0	37.0
9	37.49736810122707	39.0	37.0	39.0	35.0	39.0
10	36.95022374398692	39.0	37.0	39.0	32.0	39.0
11	36.414359313372366	38.0	35.0	39.0	32.0	39.0
12	35.102949148023434	35.0	34.0	39.0	30.0	39.0
13	34.683021992555744	35.0	33.0	39.0	29.0	39.0
14	35.309281012438916	36.0	33.0	40.0	27.0	41.0
15	35.78333020037083	36.0	34.0	40.0	30.0	41.0
16	35.95982911266512	36.0	34.0	40.0	31.0	41.0
17	35.77096530149402	35.0	34.0	40.0	31.0	41.0
18	35.889024159977055	36.0	35.0	40.0	31.0	41.0
19	35.774411139721295	36.0	34.0	40.0	31.0	41.0
20	35.52268748467687	35.0	34.0	40.0	30.0	41.0
21	35.3618958586441	35.0	34.0	40.0	30.0	41.0
22	35.187056365149516	35.0	34.0	40.0	29.0	41.0
23	35.13458870510244	35.0	34.0	39.0	29.0	41.0
24	35.28964722001093	35.0	34.0	39.0	30.0	41.0
25	35.19592748516469	35.0	34.0	39.0	30.0	41.0
26	34.942033044554094	35.0	34.0	39.0	29.0	41.0
27	34.92873078011608	35.0	34.0	39.0	29.0	41.0
28	35.04605244621292	36.0	34.0	39.0	29.0	41.0
29	35.041488413715896	36.0	34.0	39.0	29.0	41.0
30	35.008900136714914	36.0	34.0	39.0	29.0	41.0
31	34.76317158415621	35.0	34.0	39.0	29.0	41.0
32	34.63122844511281	35.0	34.0	39.0	29.0	41.0
33	34.40880030244653	35.0	33.0	39.0	27.0	41.0
34	34.24276380280251	35.0	34.0	39.0	26.0	41.0
35	34.000073172547175	35.0	33.0	39.0	24.0	41.0
36	33.7681313371526	35.0	33.0	39.0	23.0	41.0
37	33.790858982624044	35.0	33.0	39.0	24.0	41.0
38	33.83397948056743	35.0	33.0	39.0	24.0	41.0
39	33.83148068013462	35.0	33.0	39.0	24.0	41.0
40	33.476080777445695	35.0	32.0	39.0	23.0	41.0
41	33.58595440341035	35.0	33.0	39.0	23.0	41.0
42	33.56564103146378	35.0	33.0	39.0	23.0	41.0
43	33.51914954996781	35.0	33.0	39.0	23.0	41.0
44	33.50483590635764	35.0	33.0	39.0	23.0	41.0
45	33.59173923995591	35.0	33.0	39.0	23.0	41.0
46	33.60745661898255	35.0	33.0	39.0	23.0	41.0
47	33.52833817155262	35.0	33.0	39.0	23.0	41.0
48	33.5594440736755	35.0	33.0	39.0	23.0	41.0
49	33.63777276223422	35.0	33.0	39.0	24.0	41.0
50	33.39290125364759	35.0	33.0	39.0	24.0	41.0
51	33.10225442935716	35.0	32.0	38.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	0.0
11	2.0
12	2.0
13	7.0
14	12.0
15	56.0
16	186.0
17	585.0
18	1503.0
19	2937.0
20	5089.0
21	8254.0
22	12294.0
23	18111.0
24	25311.0
25	34465.0
26	44215.0
27	51699.0
28	57881.0
29	66897.0
30	80155.0
31	98118.0
32	125815.0
33	164212.0
34	294928.0
35	430773.0
36	137741.0
37	165966.0
38	234476.0
39	316120.0
40	129.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.96925407316666	24.907178100718227	30.536544010133138	12.587023815981976
2	28.825315682769254	26.973377388253116	32.044234907426215	12.157072021551418
3	27.002772566686893	26.347710056725543	34.40434392611086	12.245173450476694
4	23.220340622412415	29.92235719893807	33.69928017558047	13.158022003069043
5	20.839541435216432	32.82158808818217	32.89345698652742	13.445413490073976
6	19.73690684503947	41.62390908773599	29.28344311318069	9.355740954043855
7	88.65955883682564	4.4686138133788855	5.772262642344785	1.0995647074506893
8	91.67477241865967	2.1942091919017335	5.067198891814389	1.063819497624205
9	88.35652356387311	4.005944638660084	6.208059829911676	1.4294719675551244
10	61.05256606450706	24.009006110748754	9.916436110063286	5.021991714680894
11	53.1462302891451	16.378034610614815	21.95643205613596	8.519303044104122
12	47.856948511338175	21.46958229829924	21.45742892695824	9.216040263404349
13	17.208332755102	52.037119508011344	22.091422789715978	8.663124947170681
14	9.944527639668099	52.189772580564444	27.94392291482421	9.921776864943242
15	8.763379747436963	23.13051501277786	58.67365927077248	9.432445969012688
16	10.386170220371321	17.703509044168882	56.4657407395726	15.444579995887198
17	10.490630339440719	17.41359436588208	32.08738147834618	40.008393816331015
18	15.62877295946367	22.18970109014479	46.2472365798815	15.934289370510033
19	32.656991910228214	19.35056420659722	29.866552618420727	18.125891264753836
20	31.73775127305514	22.19865841919543	29.496232244618348	16.56735806313109
21	16.49347061176034	34.133773714318394	33.11553987251997	16.257215801401294
22	18.515808424178733	23.311890412756245	24.60986206133794	33.56243910172708
23	11.655461594715764	34.871260472820815	27.62974354704343	25.843534385419993
24	10.278892537703838	20.827219851123306	56.01051497913531	12.883372632037549
25	10.044151642113913	23.722665953444597	49.91553617183942	16.31764623260207
26	9.762058856800905	41.42722632731426	31.019440768294928	17.791274047589912
27	9.565880734635552	50.30869142674271	28.315546937455554	11.809880901166176
28	9.33597595566921	34.309892465792885	43.81959014121881	12.534541437319092
29	9.625217782947516	25.664892442663636	42.77860552469552	21.931284249693327
30	14.35666402152114	42.10613299488928	30.322577389430606	13.21462559415898
31	31.9323313740753	28.866107275159475	28.282913663543376	10.918647687221844
32	34.63336558812855	22.714272557645458	31.7404006239011	10.91196123032489
33	35.272069407945786	26.211205408376408	23.713540411641837	14.803184772035976
34	18.925112103285993	26.50986714977369	25.871247436332524	28.69377331060779
35	17.307031587411124	25.073246140253268	37.60989023697392	20.009832035361686
36	38.36196945172315	23.826747593821715	24.845444020688486	12.96583893376665
37	15.966922644422214	43.99200821214656	28.157637216398555	11.883431927032673
38	16.22138648519875	42.63162963252663	23.40398689454448	17.74299698773014
39	19.395434958226467	39.54198190787744	26.529085456703932	14.533497677192159
40	27.47755305955867	23.155704872408524	26.715170813741807	22.651571254291003
41	13.264963260232276	23.95042601982135	28.47307817982027	34.3115325401261
42	16.67392084160204	20.477253220328006	28.56412333190773	34.28470260616222
43	16.950294393342812	20.24061993127668	31.0244030444826	31.78468263089791
44	11.590321206455501	30.412739424569406	37.48234291767542	20.514596451299674
45	11.513910563802888	50.76896356974374	23.587885485804737	14.129240380648636
46	18.374131233701764	40.93183977230722	25.61392397876987	15.080105015221152
47	17.138398303406184	29.552793782520254	25.82170878083182	27.487099133241742
48	18.222865916353687	27.082925943074283	40.4599609494096	14.234247191162439
49	22.834881100918818	25.89576444495469	38.627535334139914	12.641819119986577
50	19.09677321682918	43.18967543770009	24.016996216474674	13.696555128996051
51	16.327360519037267	39.38121257003433	25.052009280297533	19.23941763063087
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3451.0
1	3747.0
2	4043.0
3	9390.5
4	14738.0
5	11919.0
6	9100.0
7	9821.0
8	10542.0
9	11417.0
10	12292.0
11	13030.5
12	13769.0
13	13164.0
14	12559.0
15	11847.5
16	11136.0
17	10844.0
18	10552.0
19	11667.5
20	12783.0
21	12223.0
22	11663.0
23	13387.5
24	15112.0
25	19919.5
26	25121.5
27	25516.0
28	35131.0
29	44746.0
30	55773.0
31	66800.0
32	76178.5
33	85557.0
34	95626.5
35	105696.0
36	105937.5
37	106179.0
38	130415.0
39	154651.0
40	214087.5
41	273524.0
42	323582.5
43	373641.0
44	357374.5
45	341108.0
46	291354.5
47	241601.0
48	203506.5
49	165412.0
50	136107.5
51	106803.0
52	80061.0
53	53319.0
54	38804.0
55	24289.0
56	18673.5
57	13058.0
58	11374.0
59	9690.0
60	8349.0
61	7008.0
62	5773.5
63	4539.0
64	3973.5
65	3408.0
66	2634.0
67	1860.0
68	1489.5
69	1119.0
70	909.0
71	699.0
72	652.5
73	606.0
74	464.0
75	242.0
76	162.0
77	117.0
78	72.0
79	47.0
80	22.0
81	33.0
82	44.0
83	28.0
84	12.0
85	9.0
86	6.0
87	5.0
88	4.0
89	2.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2377941.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.627902627587003
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.19747287134862	12.533182970119865
2	8.494703138443091	2.655087869956927
3	2.8892209346676134	1.3545739030971413
4	1.445568498872609	0.9036481496755298
5	0.8922097275199692	0.6971683372534005
6	0.5938998681682137	0.5568845586161761
7	0.4655119775040486	0.509248309948612
8	0.3510273819430393	0.43886573956980884
9	0.27074343629758085	0.3808036853565209
>10	2.689746395355154	9.115703864560297
>50	0.546810808854631	6.096832606887029
>100	0.8760302948391425	33.40997063608608
>500	0.2651606662228387	26.592127388332422
>1k	0.02162370366750978	4.448044894223896
>5k	2.7029629584387224E-4	0.30785708631627495
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7288	0.30648363437107984	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2925	0.12300557499113729	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2453	0.10315647024043068	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04655287915049196	0.0
2	0.0	0.0	0.0	0.15067657271563928	0.0
3	0.0	0.0	0.0	0.20656525960904834	0.0
4	0.0	0.0	0.0	0.3116561764989123	0.0
5	0.0	0.0	0.0	0.5238145101161047	0.0
6	0.0	0.0	0.0	0.7048955377782712	0.0
7	0.0	0.0	0.0	0.8327792825810229	0.0
8	0.0	0.0	0.0	1.133753949320021	0.0
9	0.0	0.0	0.0	1.242082961688284	0.0
10	0.0	0.0	0.0	1.446924040588055	0.0
11	0.0	0.0	0.0	1.637719354685419	0.0
12	0.0	0.0	0.0	1.8270428072016927	0.0
13	0.0	0.0	0.0	1.8916365040175513	0.0
14	0.0	0.0	0.0	1.9166581508960903	0.0
15	0.0	0.0	0.0	1.9595524026878717	0.0
16	0.0	0.0	0.0	2.0548869799545066	0.0
17	0.0	0.0	0.0	2.1755796296039303	0.0
18	0.0	0.0	0.0	2.37419683667509	0.0
19	0.0	0.0	0.0	2.456200553335848	0.0
20	0.0	0.0	0.0	2.5363118765352044	0.0
21	0.0	0.0	0.0	2.640771995604601	0.0
22	0.0	0.0	0.0	2.7554089861775375	0.0
23	0.0	0.0	0.0	2.8939321875521724	0.0
24	0.0	0.0	0.0	2.9924628071091757	0.0
25	0.0	0.0	0.0	3.074592683334027	0.0
26	0.0	0.0	0.0	3.161516622994431	0.0
27	0.0	0.0	0.0	3.248734934971053	0.0
28	0.0	0.0	0.0	3.34377513992147	0.0
29	0.0	0.0	0.0	3.448109099426773	0.0
30	0.0	0.0	0.0	3.5949167788435457	0.0
31	0.0	0.0	0.0	3.7297392996714382	0.0
32	0.0	0.0	0.0	3.850936587577236	0.0
33	0.0	0.0	0.0	3.972554407363345	0.0
34	0.0	0.0	0.0	4.0919013549957715	0.0
35	0.0	0.0	0.0	4.261922394205744	0.0
36	0.0	0.0	0.0	4.39796445748654	0.0
37	0.0	0.0	0.0	4.5441833922708765	0.0
38	0.0	0.0	0.0	4.68665959332044	0.0
39	0.0	0.0	0.0	4.823963252242171	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGTA	20	7.034809E-4	45.000004	45
CGGCGGA	25	3.8920676E-5	45.000004	4
CGTGCTA	20	7.034809E-4	45.000004	28
CCGGATA	20	7.034809E-4	45.000004	20
CCAATCG	50	2.1827873E-11	45.000004	40
GCCGGAT	20	7.034809E-4	45.000004	19
GCGCCGA	20	7.034809E-4	45.000004	32
CCGTCCC	20	7.034809E-4	45.000004	11
ATATCCG	85	0.0	45.000004	1
TCGTGAC	40	6.8212103E-9	45.000004	22
CTACGCT	40	6.8212103E-9	45.000004	21
CGGGCTA	165	0.0	45.000004	6
AACGACG	40	6.8212103E-9	45.000004	43
CTCGCTA	40	6.8212103E-9	45.000004	27
ACGCTAC	40	6.8212103E-9	45.000004	23
ACCGTAG	90	0.0	45.000004	1
TCGAGCC	20	7.034809E-4	45.000004	16
CCAACGC	20	7.034809E-4	45.000004	13
ATTGCGA	20	7.034809E-4	45.000004	1
TCGGTTA	20	7.034809E-4	45.000004	36
>>END_MODULE