FastQCFastQC Report
Thu 26 May 2016
SRR1545156_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545156_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1903640
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70250.36902985858670756No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33850.17781723435103275No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC24340.1278603097224265No Hit
TTTGATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA20320.10674287155134374No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAC207.034454E-445.0000046
GTACCAC207.034454E-445.0000049
CGAACAC207.034454E-445.00000423
GCGACGG207.034454E-445.0000042
GTCGATG207.034454E-445.0000041
GGTCGTA207.034454E-445.0000048
GATCGCT406.8193913E-945.0000049
ACGTTCG207.034454E-445.0000042
ACGTTAT207.034454E-445.00000440
GCGAACA207.034454E-445.00000422
ACACCCG207.034454E-445.0000041
CATCTCG406.8193913E-945.00000420
TAACGCA406.8193913E-945.00000428
CCTCGGG406.8193913E-945.0000043
TCTAGCG207.034454E-445.0000041
GTAATCG207.034454E-445.0000041
CCCGTCG207.034454E-445.00000437
CGCTAAT406.8193913E-945.00000425
GTCGTAA207.034454E-445.00000438
CTCGCTA406.8193913E-945.00000423