FastQCFastQC Report
Thu 26 May 2016
SRR1545155_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545155_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1615209
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61860.3829844930284564No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG19060.1180033048354733No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAG800.045.0000041
AATCCGG207.0341356E-445.0000042
TAGACGC207.0341356E-445.00000430
CTAAGCG600.045.0000041
GCGTTAG1550.045.0000041
ATCCGGA207.0341356E-445.00000412
AATGCGT207.0341356E-445.00000416
CTACGCG302.1660508E-645.0000041
TGCGAAG800.045.0000041
ACGGACC302.1660508E-645.00000439
GCCTCGT406.8175723E-945.00000413
TTACGCG750.045.0000041
CGATGCG302.1660508E-645.00000410
GCGTCAG207.0341356E-445.00000419
TGTCGTT207.0341356E-445.00000431
TATCGCT406.8175723E-945.00000444
ACCTTCG406.8175723E-945.00000423
TCCGGCA502.1827873E-1145.010
GATCGCG253.891511E-545.01
ATTACCG351.2123382E-745.01